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王曦璠,李家宇,汤婉婷,肖小芹.碱提法、酶解法提取香菇膳食纤维的工艺优化及差异性研究[J].湖南中医药大学学报英文版,2019,39(12):1525-1528.[Click to copy
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| 碱提法、酶解法提取香菇膳食纤维的工艺优化及差异性研究 |
| 王曦璠,李家宇,汤婉婷,肖小芹 |
| (湖南中医药大学, 湖南 长沙 4102018) |
| 摘要: |
| 目的 对比研究碱提法、酶解法的提取工艺，比较不同提取方法的香菇膳食纤维（可溶性膳食纤维、不溶性膳食纤维）产率以及其理化性质差异。方法 优化碱提法温度、料液比、碱液浓度和酶解法加酶量、酶解温度、酶解时间等因素，完善香菇膳食纤维最优提取条件。结果 碱提法的最优工艺条件为碱液浓度1.25%，料水比约1：20，温度80℃；酶解法的最优工艺条件为蛋白酶添加量0.4%，淀粉酶添加量0.4%。结论 碱提取法的香菇膳食纤维平均产率高于酶解法。且两种提取方法得到的膳食纤维的理化性质无明显差异，故在生产中宜采用新建立的碱提取法进行香菇膳食纤维的提取。 |
| 关键词: 香菇 水溶性膳食纤维 不溶膳食纤维 碱提法 酶解法 |
| DOI：10.3969/j.issn.1674-070X.2019.12.023 |
| Received:May 28, 2019 |
| 基金项目:湖南中医药大学研究生科研创新项目（2018CX21）；湖南中医药大学中西医结合一流学科开放基金项目（2018ZXYJH19）。 |
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| Study on Technological Optimization and Difference of Extraction of Lentinus edodes Dietary Fiber by Alkali Extraction and Enzymatic Hydrolysis |
| WANG Xifan,LI Jiayu,TANG Wanting,XIAO Xiaoqin |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To compare the extraction process of alkali extraction and enzymatic hydrolysis, and to compare the yield of dietary fiber (Soluble dietary fiber, insoluble dietary fiber) and the physicochemical properties of different extraction methods. Methods The amount of enzyme, enzymatic hydrolysis temperature, enzymatic hydrolysis time, temperature, material-liquid ratio, alkali concentration and other factors were optimized, and the alkali extraction and enzymatic extraction of dietary fiber experiment was improved to find the optimal extraction conditions. Results The optimum technological conditions of alkali extraction were alkali concentration 1.25%, ratio of material to water 1:20 and temperature 80℃. The optimum technological conditions of enzymatic hydrolysis were 0.4% of protease and 0.4% of amylase. Conclusion The average yield of dietary fiber by alkali extraction was higher than that by enzymatic hydrolysis. The physical and chemical properties of dietary fiber obtained by the 2 extraction methods were not significantly different, so a new alkali extraction method should be used to extract dietary fiber from Lentinus edodes. |
| Key words: lentinus edodes soluble dietary fiber insoluble dietary fiber alkaline collection enzymolysis method |
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