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高雯华,袁梦石,曾倩柔,范钊坤.何首乌颗粒剂对阿尔茨海默病模型大鼠学习记忆及miRNA-101表达的影响[J].湖南中医药大学学报英文版,2018,38(10):1120-1124.[Click to copy
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| 何首乌颗粒剂对阿尔茨海默病模型大鼠学习记忆及miRNA-101表达的影响 |
| 高雯华,袁梦石,曾倩柔,范钊坤 |
| (湖南中医药大学, 湖南 长沙 410208;湖南中医药大学第一附属医院, 湖南 长沙 410007;浙江省中医院, 浙江 杭州 310006) |
| 摘要: |
| 目的 研究何首乌对阿尔茨海默病模型大鼠学习记忆能力及双侧海马区miRNA-101表达的影响，并探讨其作用机制。方法 将120只SD大鼠随机分为4组，每组30只，分别为假手术组、石杉碱甲组、模型组、中药组。除假手术组外，使用化学试剂冈田酸（OA）注射SD大鼠杏仁核部位，形成阿尔茨海默病模型。造模后每组分别予以相应干预（假手术组与模型组均灌等容量生理盐水）。干预21 d结束后，运用Morris水迷宫作为行为学检测方法，评测大鼠学习记忆能力，行为学观察共进行5 d，结束后处死实验大鼠，取双侧海马运用RT-PCR进行miRNA-101半定量检测。结果 经21 d干预后，与模型组相比，中药组与石杉碱甲组的阿尔茨海默病模型大鼠的学习记忆能力均更强，其差异具有统计学意义（P<0.01）；与模型组相比，中药组与石杉碱甲组大鼠双侧海马区miRNA-101的相对表达量增加（P<0.01）。结论 何首乌能显著改善阿尔茨海默病模型大鼠的学习记忆能力，对阿尔茨海默病有一定的治疗作用；通过提高模型大鼠海马区miRNA-101的含量来改善症状可能是其作用机制之一。 |
| 关键词: 何首乌 阿尔茨海默病 学习记忆能力 miRNA-101 |
| DOI：10.3969/j.issn.1674-070X.2018.10.006 |
| Received:November 30, 2017 |
| 基金项目:湖南省中医药管理局资助课题（2015107）。 |
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| Effect of Heshouwu Granule on Learning and Memory and miRNA-101 Expression in a Rat Model of Alzheimer's Disease |
| GAO Wenhua,YUAN Mengshi,ZENG Qianrou,FAN Zhaokun |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;Zhejiang Provincial Hospital of Traditional Chinese Medicine, Hangzhou, Zhejiang 310006, China) |
| Abstract: |
| Objective To study the effect of Fallopia multiflora on learning and memory abilities and miRNA-101 expression in bilateral hippocampus in a rat model of Alzheimer's disease, and to explore its mechanism. Methods A total of 120 Sprague-Dawley rats were randomly and equally divided into four groups:sham-operation group, control (huperzine A) group, model group, and traditional Chinese medicine (TCM) group. To establish a model of Alzheimer's disease, okadaic acid was injected into the amygdala of rats in all the groups except for the sham-operation group. Each group received the corresponding intervention after model establishment (the sham-operation group and the model group were given the same amount of normal saline). After 21 days of intervention, Morris water maze was used for the behavioral test to evaluate the learning and memory abilities of rats. After 5 days of behavioral observation, the rats were sacrificed and the bilateral hippocampus was collected for semi-quantitative measurement of miRNA-101 using RT-PCR. Results After 21 days of intervention, the TCM group and the control group had significantly better learning and memory abilities and significantly higher relative expression of miRNA-101 in the bilateral hippocampus than the model group (all P<0.01). Conclusion Fallopia multiflora can substantially improve the learning and memory abilities of the rat model of Alzheimer's disease, suggesting a certain therapeutic effect against Alzheimer's disease. The symptoms are improved probably by increasing the content of miRNA-101 in the hippocampus of rats. |
| Key words: Fallopia multiflora Alzheimer's disease learning and memory abilities miRNA-101 |
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