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王秀兰,洪宗超,段雪云.复方苦参汤有效部位指纹图谱研究[J].湖南中医药大学学报英文版,2018,38(7):738-742.[Click to copy
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| 复方苦参汤有效部位指纹图谱研究 |
| 王秀兰,洪宗超,段雪云 |
| (湖北省中医院, 湖北 武汉 430061;湖北中医药大学中药资源与中药化学湖北省重点实验室, 湖北 武汉 430061) |
| 摘要: |
| 目的 建立复方苦参汤有效部位的指纹图谱，为控制相关制剂质量提供科学依据。方法 采用HPLC，色谱柱为Agilent 5 TC-C18（2）柱（250mm×4.6mm，5μm）；流动相A为乙腈，B为超纯水，梯度洗脱：0～2 min，10%～20% A；2～10 min，20%～40% A；10～15 min，40%～60% A；15～35 min，60%～65% A；35～40 min，65%～10% A；柱温为25℃；体积流量1 mL/min；检测波长220 nm；进样量为10 μL。结果 10批不同样品有效部位的图谱，相似度较好（均在0.88以上）；得到了26个共有峰，指认了苦参碱、氧化苦参碱、甘草苷三种成分。结论 本研究建立了复方苦参汤有效部位的指纹图谱，此法简便可靠，重现性好，为相关新药的开发利用提供实验依据。 |
| 关键词: 指纹图谱 复方苦参汤 有效部位 高效液相色谱法 |
| DOI：10.3969/j.issn.1674-070X.2018.07.005 |
| Received:November 03, 2017 |
| 基金项目:国家自然科学基金面上项目（81573784）；中国中医科学院中医基础理论研究所自主选题项目（YZ-1605）。 |
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| A Study on the Fingerprint of the Effective Part of Compound Kushen Decoction |
| WANG Xiulan,HONG Zongchao,DUAN Xueyun |
| (Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, Hubei 430061, China;Hubei Key Laboratory of Resources and Chemistry of Chinese Medicine, Hubei University of Chinese Medicine, Wuhan, Hubei 430061, China) |
| Abstract: |
| Objective To establish the fingerprint of the effective part of Compound Kushen Decoction, and to provide a scientific basis for the quality control of the preparation. Methods High-performance liquid chromatography was performed on an Agilent 5 TC-C18(2) column (250 mm×4.6 mm, 5 μm) with acetonitrile as mobile phase A and ultrapure water as mobile phase B for gradient elution (0-2 min, 10%-20% A; 2-10 min, 20%-40% A; 10-15 min, 40%-60% A; 15-35 min, 60%-65% A; 35-40 min, 65%-10% A) at a column temperature of 25℃, a flow rate of 1 ml/min, a detection wavelength of 220 nm, and an injection volume of 10 μL. Results The fingerprints of the effective parts of 10 batches of samples were obtained, and a good similarity was achieved (>0.88). A total of 26 common peaks were obtained, and the three components of matrine, oxymatrine, and liquiritin were identified. Conclusion The fingerprint of the effective part of Compound Kushen Decoction is established in this study. The method is simple and reliable and has good reproducibility, which provides an experimental basis for the development and application of related new drugs. |
| Key words: fingerprint Compound Kushen Decoction effective part high-performance liquid chromatography |
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