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王英,彭俊,刘家琪,徐剑,谭涵宇,彭清华.滋阴明目丸对RCS大鼠视网膜Bax及Caspase-3表达的影响[J].湖南中医药大学学报英文版,2018,38(7):732-737.[Click to copy
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| 滋阴明目丸对RCS大鼠视网膜Bax及Caspase-3表达的影响 |
| 王英,彭俊,刘家琪,徐剑,谭涵宇,彭清华 |
| (湖南中医药大学, 湖南 长沙 410208;湖南中医药高等专科学校附属第一医院, 湖南 株洲 412008;湖南中医药大学第一附属医院, 湖南 长沙 410007;湖南中医药大学, 湖南 长沙 410208;海南省眼科医院中山大学中山眼科中心, 海南 海口 570000;湖南中医药大学, 湖南 长沙 410208;宁波博视眼科医院, 浙江 宁波 315000;湖南中医药大学, 湖南 长沙 410208;湖南中医药大学第一附属医院, 湖南 长沙 410007) |
| 摘要: |
| 目的 观察滋阴明目丸对皇家外科学院（royal college surgeons，RCS）大鼠视网膜Bax及Caspase-3表达的影响。方法 实验对象分3组，分别为：空白组、模型组、滋阴明目丸组（每组雌雄各4只共8只）。空白组：RCS（rdy+/+，p+/+）大鼠，灌胃生理盐水；模型组：RCS（rdy-/-，p-/-）大鼠，灌胃生理盐水；滋阴明目丸组：RCS（rdy-/-，p-/-）大鼠，灌胃滋阴明目丸混悬液。灌胃30 d后，免疫组化法检测各组大鼠视网膜组织中Bax及Caspase-3表达，RT-qPCR法及Western Blot法分别测定各组视网膜组织中Bax及Caspase-3 mRNA及蛋白相对表达水平。结果 Bax、Caspase-3蛋白在滋阴明目丸组视网膜中的阳性表达显著低于模型组，但高于空白组。与空白组比较，模型组Bax、Caspase-3平均光密度值均增加，滋阴明目丸组Bax平均光密度值增加（P<0.05或P<0.01）；与模型组比较，滋阴明目丸组Bax、Caspase-3平均光密度值均显著减少（P<0.01）。与空白组比较，模型组Bax、Caspase-3 mRNA、蛋白表达均增加，滋阴明目丸组Bax mRNA、蛋白表达均增加（P<0.05或P<0.01）；与模型组比较，滋阴明目丸组Bax、Caspase-3 mRNA、蛋白表达均显著减少（P<0.01）。结论 （1）滋阴明目丸RCS（rdy-/-，p-/-）大鼠视网膜的超微结构具有保护作用；（2）滋阴明目丸能抑制RCS（rdy-/-，p-/-）大鼠视网膜上Bax及Caspase-3的表达；（3）滋阴明目丸可能是通过下调视网膜上Bax及Caspase-3的表达，从而减轻视网膜感光细胞的凋亡，达到保护视细胞的目的。 |
| 关键词: 滋阴明目丸 RCS大鼠 Bax Caspase-3 细胞凋亡 |
| DOI：10.3969/j.issn.1674-070X.2018.07.004 |
| Received:March 12, 2018 |
| 基金项目:湖南中医药大学中医诊断学国家重点学科开放基金优先资助项目（2015ZYZD02）；湖南省教育厅创新平台开放基金项目（16K065）；湖南省高层次卫生人才“225”工程培养项目；国家中医药管理局中医眼科学重点学科建设项目；湖南省中医五官科学重点学科建设项目。 |
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| Effect of Ziyin Mingmu Pill on the Expression of Bax and Caspase-3 in the Retina in Royal College Surgeons Rats |
| WANG Ying,PENG Jun,LIU Jiaqi,XU Jian,TAN Hanyu,PENG Qinghua |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The First Affiliated Hospital of Hunan Traditional Chinese Medical College, Zhuzhou, Hunan 412008, China;The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hainan Eye Hospital & Zhongshan Ophthalmic Center, Sun Yat-Sen University, Haikou, Hainan 570000, China;Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Ningbo Boshi Eye Hospital, Ningbo, Zhejiang 315000, China;Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China) |
| Abstract: |
| Objective To investigate the effect of Ziyin Mingmu Pill on the expression of Bax and Caspase-3 in the retina in Royal College Surgeons (RCS) rats. Methods RCS rats were divided into blank group, model group, and Ziyin Mingmu Pill group, with 8 rats (4 male and 4 female rats) in each group. The RCS (rdy+/+, p+/+) rats in the blank group were treated with normal saline by gavage; the RCS (rdy-/, p-/-) rats in the model group were given normal saline by gavage; the RCS (rdy-/, p-/-) rats in the Ziyin Mingmu Pill group were given the suspension of Ziyin Mingmu Pill by gavage. After 30 days of intragastric administration, immunohistochemistry was used to measure the expression of Bax and Caspase-3 in the retina, and RT-qPCR and Western blot were used to measure the mRNA and protein expression of Bax and Caspase-3 in the retina. Results The Ziyin Mingmu Pill group had significantly lower expression of Bax and Caspase-3 in the retina than the model group and significantly higher expression of Bax and Caspase-3 than the blank group. Compared with the blank group, the model group had significant increases in the mean optical density of Bax and caspase-3, and the Ziyin Mingmu Pill group had a significant increase in the mean optical density of Bax (P<0.05 or P<0.01); compared with the model group, the Ziyin Mingmu Pill group had significant reductions in the mean optical density of Bax and Caspase-3 (P<0.01). Compared with the blank group, the model group had significant increases in the mRNA and protein expression of Bax and Caspase-3, and the Ziyin Mingmu Pill group had significant increases in the mRNA and protein expression of Bax (P<0.05 or P<0.01); compared with the model group, the Ziyin Mingmu Pill group had significant reductions in the mRNA and protein expression of Bax and Caspase-3 (P<0.01). Conclusion Ziyin Mingmu Pill has a protective effect on the ultrastructure of the retina in RCS (rdy-/, p-/-) rats and can inhibit the expression of Bax and Caspase-3 in the retina. Ziyin Mingmu Pill protects the visual cells by downregulating the expression of Bax and Caspase-3 in the retina and reducing the apoptosis of photoreceptor cells in the retina. |
| Key words: Ziyin Mingmu Pill Royal College Surgeons rat Bax Caspase-3 cell apoptosis |
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