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丁强盛, 吴庆泽, 瞿启睿, 张亮, 刘梨, 张泓, 艾坤, 祁芳.电针对佐剂性关节炎大鼠TKS5蛋白表达及Th1、Th17的影响[J].湖南中医药大学学报英文版,2025,45(2):267-273.[Click to copy
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| 电针对佐剂性关节炎大鼠TKS5蛋白表达及Th1、Th17的影响 |
| 丁强盛,吴庆泽,瞿启睿,张亮,刘梨,张泓,艾坤,祁芳 |
| (湖南中医药大学针灸推拿与康复学院, 湖南 长沙 410208;湖南中医药大学继续教育学院, 湖南 长沙 410208;湖南中医药大学第一附属医院, 湖南 长沙 410208) |
| 摘要: |
| 目的 观察电针对佐剂性关节炎（AIA）大鼠关节滑膜组织中酪氨酸激酶底物5（TKS5）蛋白表达及辅助性T细胞1（Th1）、辅助性T细胞17（Th17）主要标记物γ-干扰素（INF-γ）、白细胞介素17A（IL-17A）的影响，探讨其可能作用机制。方法 将36只SPF级SD大鼠按体质量分层后再随机分为空白组、模型组、电针组、药物组，每组9只；采用不完全弗式佐剂建立大鼠模型，于造模后第1天对电针组大鼠进行电针足三里、关元、阿是穴干预，连续干预21 d；药物组给予甲氨蝶呤灌胃给药，每周2次，连续干预3周，共6次。采用足趾容积测量仪检测大鼠后肢足趾容积；HE染色观察关节滑膜组织病理形态改变；Western blot检测滑膜组织中TKS5的蛋白表达；免疫荧光检测滑膜组织中丝状肌动蛋白（F-actin）、皮层肌动蛋白（Cortactin）和INF-γ、IL-17A的阳性率。结果 与空白组比较，模型组大鼠足趾容积显著增大（P<0.01），HE染色结果显示模型组滑膜组织增生、炎性细胞大量浸润，滑膜组织中TKS5蛋白表达显著升高（P<0.01），滑膜组织中F-actin、Cortactin、INF-γ、IL-17A阳性表达显著增高（P<0.01）。与模型组比较，电针组、药物组大鼠足趾容积减小（P<0.05，P<0.01），HE染色结果可见两组滑膜组织炎性浸润情况明显改善，滑膜组织中TKS5蛋白表达均降低（P<0.05，P<0.01），滑膜组织中F-actin、Cortactin、INF-γ、IL-17A阳性表达均降低（P<0.05，P<0.01）。结论 电针改善AA模型大鼠关节滑膜炎症，其机制可能与调节TKS5蛋白表达进而抑制炎性效应T细胞Th1、Th17的高度运动有关。 |
| 关键词: 佐剂性关节炎 电针 酪氨酸激酶底物5 辅助性T细胞1 辅助性T细胞17 丝状肌动蛋白 皮层肌动蛋白 |
| DOI：10.3969/j.issn.1674-070X.2025.02.010 |
| Received:September 22, 2024 |
| 基金项目:国家自然科学基金项目（82074565）；湖南省自然科学基金青年项目（2022JJ40304）。 |
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| Effects of electroacupuncture on TKS5 protein expression, Th1, and Th17 in rats with adjuvant arthritis |
| DING Qiangsheng, WU Qingze, QU Qirui, ZHANG Liang, LIU Li, ZHANG Hong, AI Kun, QI Fang |
| (School of Acupuncture-moxibustion, Tuina and Rehabilitation, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;School of Continuing Education, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To observe the effects of electroacupuncture on tyrosine kinase substrate 5 (TKS5) protein expression and the key markers of T helper 1 (Th1) and T helper 17 (Th17) cells, namely interferon gamma (INF-γ) and interleukin-17A (IL-17A), in the synovial tissue of rats with adjuvant-induced arthritis (AIA), and to explore its possible mechanism of action. Methods A total of 36 SPF grade SD rats were stratified by body weight and then randomly divided into blank group, model group, electroacupuncture group, and medication group, with nine rats in each group. Rat models were established using incomplete Freund's adjuvant. On the first day after modeling, electroacupuncture was applied to the electroacupuncture group at "Zusanli" (ST36), "Guanyuan" (CV4), and "Ashi points" for 21 consecutive days. Rats in the medication group were gavaged with methotrexate twice a week for three weeks, for a total of six doses. The hind toe volume of rats was measured using a paw volume meter. HE staining was used to observe the histopathological changes in synovial tissue of joints; Western blot was used to determine TKS5 protein expression in the synovial tissue; and immunofluorescence was used to measure the positive rates of filamentous actin (F-actin), cortactin, IFN-γ, and IL-17A in synovial tissue. Results Compared with the blank group, the toe volume of rats in the model group significantly increased (P<0.01). HE staining showed synovial tissue hyperplasia and massive inflammatory cell infiltration in the model group. The TKS5 protein expression in the synovial tissue significantly increased (P<0.01), and the positive expressions of F-actin, cortactin, INF-γ, and IL-17A in the synovial tissue also significantly increased (P<0.01). Compared with the model group, the toe volume of rats in the electroacupuncture group and medication group decreased (P<0.05, P<0.01). HE staining showed significantly reduced inflammatory infiltration of synovial tissue in both groups, as well as reduced TKS5 protein expression (P<0.05, P<0.01) and the positive expressions of F-actin, cortactin, INF-γ, and IL-17A in synovial tissue (P<0.05, P<0.01). Conclusion Electroacupuncture reduces synovial inflammation in AA rat models, and its mechanism may be related to regulating TKS5 protein expression, thereby inhibiting the hypermotility of inflammatory effector T cells, including Th1 and Th17. |
| Key words: adjuvant-induced arthritis electroacupuncture tyrosine kinase substrate 5 T helper 1 cells T helper 17 cells filamentous actin cortactin |
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