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唐青, 洪燕玲, 苏丽红, 郭榕蕙, 林丽莉.捏脊疗法对孤独症谱系障碍大鼠不同脑区BDNF及CREB蛋白表达的影响[J].湖南中医药大学学报英文版,2025,45(2):259-266.[Click to copy
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| 捏脊疗法对孤独症谱系障碍大鼠不同脑区BDNF及CREB蛋白表达的影响 |
| 唐青,洪燕玲,苏丽红,郭榕蕙,林丽莉 |
| (福建中医药大学针灸推拿学院, 福建 福州 350122;福建省中医药科学院, 福建 福州 350001) |
| 摘要: |
| 目的 探讨捏脊疗法干预孤独症谱系障碍（ASD）模型大鼠对其前额叶、海马及小脑区域中脑源性神经营养因子（BDNF）、环磷酸腺苷反应元件结合蛋白（CREB）表达的影响。方法 将ASD模型大鼠随机分为模型组、捏脊组，每组6只；同时随机选取6只正常大鼠为空白组。捏脊组在大鼠23日龄开始捏脊疗法，21次/d，连续干预28 d；空白组和模型组仅模拟抓取和固定。干预结束次日完成旷场实验，记录活动行为数据（总路程、站立次数、理毛次数），并于旷场实验次日取材。采用免疫组织化学法和Western blot检测前额叶、海马和小脑区域中BDNF及CREB蛋白表达。结果 与空白组相比，模型组活动总路程增长、站立次数减少、理毛次数增多（P<0.01）；与模型组相比，捏脊组活动总路程减少、站立次数增加、理毛次数减少（P<0.05）。与空白组比较，模型组前额叶区、海马区、小脑区BDNF、CREB蛋白表达均减少（P<0.05，P<0.01）；与模型组比较，捏脊组前额叶区、海马区、小脑区BDNF、CREB蛋白表达均增加（P<0.05，P<0.01）。与空白组相比，模型组前额叶区、海马区、小脑区BDNF、CREB蛋白阳性平均光密度均减少（P<0.05，P<0.01）；与模型组相比，捏脊组前额叶区、海马区、小脑区BDNF、CREB蛋白阳性平均光密度均增加（P<0.05，P<0.01）。结论 捏脊疗法能够有效改善ASD大鼠的行为表征，其作用机制可能与上调ASD大鼠前额叶、海马、小脑区的BDNF、CREB蛋白表达水平有关。 |
| 关键词: 孤独症谱系障碍 捏脊 督脉 多脑区 脑源性神经营养因子 环磷酸腺苷反应元件结合蛋白 脑神经发育 |
| DOI：10.3969/j.issn.1674-070X.2025.02.009 |
| Received:July 11, 2024 |
| 基金项目:国家自然科学基金青年项目（81704194）；福建省自然科学基金项目（2022J01366）；福建省财政厅教育和科研专项项目（x2020002-财政专项）；福建省属公益类科研院所基本科研专项项目（2023R1003006）。 |
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| Effects of spine-pinching manipulation on protein expressions of BDNF and CREB in different brain regions of autistic spectrum disorder rats |
| TANG Qing, HONG Yanling, SU Lihong, GUO Ronghui, LIN Lili |
| (School of Acupuncture&Moxibustion and Tuina, Fujian University of Traditional Chinese Medicine, Fuzhou, Fujian 350122, China;Fujian Academy of Chinese Medical Sciences, Fuzhou, Fujian 350001, China) |
| Abstract: |
| Objective To investigate the effects of spine-pinching manipulation on the expressions of brain derived neurotrophic factor (BDNF) and cyclic adenosine phosphate response element binding protein (CREB) in the prefrontal, hippocampal, and cerebellar regions of autism spectrum disorder (ASD) model rats. Methods The model rats with ASD were randomly divided into model group and spine-pinching manipulation group, with six rats in each group. Additionally, six normal rats were randomly selected as blank group. The spine-pinching manipulation group received spine-pinching manipulation starting from postnatal day 23, 21 times/day, for a continuous intervention of 28 days. The blank and model groups underwent simulated grasping and fixing only. The open field test was conducted the day after the intervention ended, and the activity behavior data (total distance, standing times, and grooming times) were recorded, and the samples were collected on the day after the open field test. The protein expressions of BDNF and CREB in the prefrontal, hippocampus, and cerebellar regions were checked by immunohistochemistry and Western blot. Results Compared with the blank group, the total distance of activity increased, standing times decreased, and grooming times increased in the model group (P<0.01). Compared with the model group, the total distance of activity in spine-pinching manipulation group decreased, the standing times increased, and the grooming times decreased (P<0.05). Compared with the blank group, the protein expressions of BDNF and CREB in the prefrontal, hippocampus, and cerebellar regions were lower in the model group (P<0.05, P<0.01). Compared with the model group, the protein expressions of BDNF and CREB in the prefrontal, hippocampus, and cerebellar regions of the spine-pinching manipulation group increased (P<0.05, P<0.01). Compared with the blank group, the average optical density of BDNF and CREB proteins in the prefrontal, hippocampus, and cerebellar regions of the model group decreased (P<0.05, P<0.01). Compared with the model group, the average optical density of BDNF and CREB proteins in the prefrontal, hippocampus, and cerebellar regions of the spine-pinching manipulation group increased (P<0.05, P<0.01). Conclusion Spine-pinching manipulation can effectively improve the behavioral characteristics of ASD rats, and its mechanism of action may be related to the upregulation of BDNF and CREB protein expressions in the prefrontal, hippocampus, and cerebellar regions of ASD rats. |
| Key words: autism spine-pinching manipulation Du meridian multiple brain regions brain derived neurotrophic factor cyclic adenosine phosphate response element binding protein cranial nerve development |
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