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李芊,欧阳里知,刘惠娟,彭涵,刘红华,葛君芸,常小荣,刘迈兰.隔药饼灸对高脂血症兔IGF-1/Sp1蛋白及基因表达的影响[J].湖南中医药大学学报英文版,2022,42(10):1688-1694.[Click to copy
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| 隔药饼灸对高脂血症兔IGF-1/Sp1蛋白及基因表达的影响 |
| 李芊,欧阳里知,刘惠娟,彭涵,刘红华,葛君芸,常小荣,刘迈兰 |
| (湖南中医药大学针灸推拿学院, 湖南 长沙 410208;湖南中医药大学护理学院, 湖南 长沙 410208) |
| 摘要: |
| 目的 探究隔药饼灸对高脂血症兔胰岛素样生长因子1（insulin-like growth factor 1, IGF-1）/特异性蛋白1（specificity protein 1, Sp1）蛋白及基因表达的影响。方法 将36只雄性新西兰兔随机分为正常组、模型组和隔药饼灸组，每组12只。造模各组予高胆固醇配方饲料喂养8周制备高脂血症模型，正常组普食饲养。模型组捆绑操作，隔药饼灸组隔药饼灸干预，施灸穴位分为腹背两组，腹组为“巨阙”与双侧“天枢”“丰隆”，背组为双侧“心俞”“肝俞”“脾俞”，每天只施灸一组穴位，两组穴位交替施灸，持续8周。干预8周后，取各组兔血清、主动脉组织及肝组织样本，采用ELISA、RT-PCR技术检测IGF-1、Sp1指标。结果 （1）干预8周后，与正常组比较，模型组血清IGF-1、Sp1含量上升（P<0.01）；与模型组比较，隔药饼灸组血清IGF-1、Sp1含量下降（P<0.01）。（2）与正常组比较，模型组主动脉IGF-1的mRNA表达量显著上升（P<0.001），与模型组比较，隔药饼灸组主动脉IGF-1的mRNA表达量下降（P<0.01）。（3）与正常组比较，模型组肝组织IGF-1和Sp1的mRNA表达量显著上升（P<0.001，P<0.01）；与模型组比较，隔药饼灸组肝组织IGF-1和Sp1的mRNA表达量下降（P<0.01）。结论 隔药饼灸可能影响IGF-1/Sp1信号通路，通过胆固醇逆转运环节，调节LDL-C的摄取和转运而缓解高脂血症。 |
| 关键词: 高脂血症 隔药饼灸 胰岛素样生长因子1 特异性蛋白1 胆固醇逆转运 低密度脂蛋白胆固醇 IGF-1/Sp1信号通路 血脂 |
| DOI：10.3969/j.issn.1674-070X.2022.10.016 |
| Received:March 21, 2022 |
| 基金项目:国家自然科学基金项目（82074559）；湖南省自然科学基金项目(2018JJ3097, 2020JJ5414,2021JJ40401)；湖南省院士专家工作站（石学敏）开放基金项目（2019YSZJJ10）；湖南省教育厅优秀青年基金项目（19B435，19B428）；长沙市杰出创新青年培养计划项目（kq1905036）；湖南省大学生研究性学习和创新性实验计划（2018-15）。 |
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| Effects of herbal cake-separated moxibustion on IGF-1/Sp1 protein and gene expression in hyperlipidemia rabbits |
| LI Qian,OUYANG Lizhi,LIU Huijuan,PENG Han,LIU Honghua,GE Junyun,CHANG Xiaorong,LIU Mailan |
| (College of Acu-moxibustion and Massage, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Nursing College, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To investigate the effects of herbal cake-separated moxibustion on the expressions of insulin-like growth factor 1 (IGF)/specificity protein 1 (Sp1) protein and gene expression in rabbits with hyperlipidemia. Methods A total of 36 male New Zealand rabbits were randomly divided into normal group, model group and herbal cake-separated moxibustion group, with 12 rabbits in each. The hyperlipidemia models were prepared in each modeling group by feeding with high cholesterol formula for 8 weeks. The normal group was fed with common food; the model group was bundled; and the herbal cake-separated moxibustion group was treated with herbal cake-separated moxibustion with the applied acupoints divided into the abdominal and dorsal groups. "Juque" (CV14), bilateral "Tianshu" (S25) and “Fenglong” (S40) were chosen for the abdominal groups, and bilateral "Xinshu" (BL15), “Ganshu” (BL18) and “Pishu” (BL20) for the back group. Moxibustions were applied only to one group of acupoints per day, and the two groups of acupoints were alternately applied for 8 weeks. After 8 weeks of intervention, serum, aortic tissue and liver tissue samples were taken from each group, and the IGF-1/Sp1 indicators were detected by ELISA, RT-PCR techniques. Results (1) After 8 weeks of intervention, compared with the normal group, the levels of serum IGF-1/Sp1 increased in the model group (P<0.01); compared with the model group, the serum IGF-1/Sp1 content in the herbal cake-separated moxibustion group decreased (P<0.01). (2) Compared with the normal group, the mRNA expression of IGF-1 in the aorta of the model group was significantly reduced (P<0.001). Compared with the model group, the mRNA expression of IGF-1 in the aorta of the herbal cake-separated moxibustion group decreased (P<0.01). (3) Compared with the normal group, the mRNA expression of IGF-1/Sp1 in liver tissues of the model group increased significantly (P<0.001, P<0.01); compared with the model group, the mRNA expression levels of IGF-1/Sp1 in liver tissues of herbal cake-separated moxibustion group decreased (P<0.01). Conclusion Herbal cake-separated moxibustion may affect IGF-1/Sp1 signaling pathway, and regulate the intake and transport of low density lipoprotein cholesterol through cholesterol reverse transport, thus alleviating hyperlipidemia. |
| Key words: hyperlipidemia herbal cake-separated moxibustion insulin-like growth factor 1 specificity protein 1 cholesterol reversal low density lipoprotein cholesterol IGF-1/Sp1 signaling pathway blood lipid |
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