| Quote
: |
汪红娟,唐红,江姗姗,李展富,吕倩忆,田浩梅,陈楚淘.针刺对脑缺血再灌注损伤大鼠海马组织铁死亡的影响[J].湖南中医药大学学报英文版,2022,42(10):1683-1687.[Click to copy
] |
|
| |
|
|
| This paper
：Browser 1478times Download 897times |
| 针刺对脑缺血再灌注损伤大鼠海马组织铁死亡的影响 |
| 汪红娟,唐红,江姗姗,李展富,吕倩忆,田浩梅,陈楚淘 |
| (湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 探讨针刺对脑缺血再灌注损伤（cerebral ischemia reperfusion injury, CIRI）大鼠的脑保护作用及缺血侧海马组织铁死亡的影响。方法 65只健康SD大鼠随机分成假手术组、模型组、针刺组、药物组，药物组5只，其他每组20只。线栓法制备大鼠脑缺血模型，2 h后拔出线栓约10 mm，建立CIRI模型。大鼠生命体征稳定后，药物组采用依达拉奉水溶液（5 mg/kg）腹腔注射后捆绑30 min；假手术组、模型组注射同等药物剂量生理盐水，并捆绑30 min；针刺组注射同等药物剂量生理盐水并选取“人中”“大椎”“百会”穴进行捆绑针刺，留针30 min。均1次/12 h，共7次。采用改良Garcia评分观察大鼠神经功能损伤程度；TTC染色检测脑梗死面积；铁离子、活性氧（reactive oxygen species, ROS）及丙二醛（malondialdehyde, MDA）试剂盒检测海马组织铁离子、ROS及MDA含量。结果 （1）干预前，与假手术组相比，其余各组改良Garcia评分显著降低（P<0.01）。干预后，与假手术组相比，模型组改良Garcia神经功能评分明显降低，脑梗死面积显著扩大（P<0.01）；与模型组相比，针刺组、药物组改良Garcia神经功能评分升高，脑梗死面积缩小（P<0.05，P<0.01）。与干预前相比，针刺组、药物组改良Garcia神经功能评分升高（P<0.05）。（2）与假手术组相比，模型组海马组织铁离子、ROS及MDA含量均明显增加（P<0.01）；与模型组相比，针刺组海马组织铁离子、ROS及MDA含量均明显降低（P<0.01）。结论 针刺可以抑制CIRI后大鼠神经细胞铁死亡，发挥脑保护作用，可能与调节神经细胞铁离子代谢、减轻海马组织铁离子集聚、降低脂质过氧化水平有关。 |
| 关键词: 针刺 铁死亡 脑缺血再灌注损伤 铁代谢 脂质过氧化 大鼠 |
| DOI：10.3969/j.issn.1674-070X.2022.10.015 |
| Received:March 21, 2022 |
| 基金项目:国家自然科学基金项目（81874508，82274662）；湖南省自然科学基金项目（2020JJ4065，2021JJ30490）；湖南省长沙市科技局自然科学基金项目（kq2014094）；湖南中医药大学研究生创新课题（2021CX39）。 |
|
| Effects of acupuncture on ferroptosis of hippocampal tissues in rats with cerebral ischemia reperfusion injury |
| WANG Hongjuan,TANG Hong,JIANG Shanshan,LI Zhanfu,LV Qianyi,TIAN Haomei,CHEN Chutao |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To explore the effects of acupuncture on brain protection and ferroptosis of ischemia hippocampal tissue in rats with cerebral ischemia reperfusion injury (CIRI). Methods A total of 65 healthy SD rats were randomly divided into the sham-operated group, model group, acupuncture group and drug group, with 5 rats in drug group and 20 rats in other groups. The rat models of cerebral ischemia were established by suture-occluded method. After 2 h, the threads were pulled out by 10 mm, and the CIRI models were thus established. With the vital signs of rats being stable, the drug group was administered with edaravone (5 mg/kg) by intraperitoneal injection and then bound for 30 min; the sham-operated group and model group were injected with the same dose of normal saline and bound for 30 min; the acupuncture group was injected with the same dose of normal saline, then bound and punctured at the acupoints of "Renzhong" (GV26), "Dazhui" (GV14), and "Baihui" (DU20), with the needle retention for 30 min, once every 12 h, for totally 7 times. The modified Garcia scores were used to observe the degrees of neurological deficits; TTC staining was used to detect cerebral infarct areas; iron ions, reactive oxygen species (ROS) and malondialdehyde (MDA) kits were used to check the content of iron ion, ROS and MDA in the hippocampal tissues. Results (1) Before the intervention, compared with the sham-operated group, the other groups showed a significant decrease in the modified Garcia scores (P<0.01). After the intervention, compared with the sham-operated group, the model group showed a significant decrease of modified Garcia scores and the marked enlargement of the cerebral infarction areas (P<0.01); compared with the model group, the acupuncture group and drug group showed the significantly higher modified Garcia scores and the decreased cerebral infarction areas (P<0.01, P<0.05). Comparing with those before intervention, the modified Garcia scores increased (P<0.05) in the acupuncture group and drug group. (2) Compared with the sham-operated group, the model group showed the significant higher contents of iron ion, ROS and MDA in hippocampal tissues (P<0.01). Compared with the model group, the acupuncture group showed the significant lower contents of iron ions, ROS and MDA in hippocampal tissues (P<0.01). Conclusion Acupuncture may inhibit the ferroptosis of nerve cells in rats after CIRI and play a role of brain protection, which may be related to the decreasing deposition of iron ions in hippocampal tissues and the decreasing levels of lipid peroxidation by regulating the iron ion metabolism of nerve cells. |
| Key words: acupuncture ferroptosis cerebral ischemia reperfusion injury iron metabolism lipid peroxidation rats |
|
二维码（扫一下试试看！） |
|
|
|
|
