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李益亮,姚金龙,梁爽,孙绍裘.桃红四物汤对兔软骨损伤模型noggin蛋白表达的影响——骨伤名师孙达武学术思想与临床经验研究（四）[J].湖南中医药大学学报英文版,2022,42(5):715-719.[Click to copy
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| 桃红四物汤对兔软骨损伤模型noggin蛋白表达的影响——骨伤名师孙达武学术思想与临床经验研究（四） |
| 李益亮,姚金龙,梁爽,孙绍裘 |
| (湖南中医药大学第二附属医院, 湖南 长沙 410005;湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 观察桃红四物汤对兔膝关节软骨损伤模型noggin蛋白表达的影响，探讨桃红四物汤对兔膝关节软骨损伤修复的分子作用机制。方法 从40只新西兰兔中随机挑选10只为假手术组，其余新西兰兔根据改良Hulth法进行右膝关节软骨损伤造模后，随机分为模型组、桃红四物汤组、氨基葡萄糖组，每组10只。假手术组做同样切口切至软骨面，但不予以造模而直接缝合。术后各组分别以等量蒸馏水、桃红四物汤、氨基葡萄糖进行灌胃。干预4、6周后，每组随机抽取5只新西兰兔，麻醉后进行腹主动脉采血，并循原手术切口暴露软骨，大体观察后截取包含股骨下端在内的软骨关节面，采用ELISA法检测noggin蛋白表达量；Western blot检测软骨组织中noggin的蛋白表达水平，并进行数据统计分析。结果 干预4、6周后，桃红四物汤组noggin蛋白的表达均低于模型组，高于假手术组(P<0.05)；与氨基葡萄糖组比较，桃红四物汤组noggin蛋白的表达更低，差异有统计学意义(P<0.05)。干预6周后，模型组、桃红四物汤组、氨基葡萄糖组关节面均不平整，但桃红四物汤组、氨基葡萄糖组软骨修复情况优于模型组。结论 桃红四物汤能抑制noggin蛋白的表达，促进膝关节软骨修复，且抑制noggin蛋白表达的效果较氨基葡萄糖更优。 |
| 关键词: 软骨损伤修复 桃红四物汤 氨基葡萄糖 noggin蛋白 新西兰兔 |
| DOI：10.3969/j.issn.1674-070X.2022.05.004 |
| Received:November 25, 2021 |
| 基金项目:湖南省科技创新计划项目（2018SK51103）；湖南省中医骨伤临床医学研究中心项目（2020SK4013）。 |
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| Effect of Taohong Siwu Decoction on noggin protein expression in rabbit cartilage injury model: Study on the academic thoughts and clinical experience of famous orthopaedic teacher Sun Dawu (4) |
| LI Yiliang,YAO Jinlong,LIANG Shuang,SUN Shaoqiu |
| (The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410005, China;Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To observe the effect of Taohong Siwu Decoction on the expression of noggin protein in a rabbit knee cartilage injury model, and to explore the molecular mechanism of Taohong Siwu Decoction on the repair of knee cartilage injury in rabbits. Methods 10 New Zealand rabbits were randomly selected from 40 New Zealand rabbits as the sham operation group. The remaining New Zealand rabbits were randomly divided into model group, Taohong Siwu Decoction group and glucosamine group, with 10 rabbits in each group, after the right knee articular cartilage injury model was established according to the modified Hulth method. In the sham operation group, the same incision was made to cut to the cartilage surface, but it was directly sutured without modeling. After operation, each group was given the same amount of distilled water, Taohong Siwu Decoction and glucosamine by gavage respectively. After 4 and 6 weeks of intervention, 5 New Zealand rabbits were randomly selected from each group. After anesthesia, abdominal aortic blood was collected, and the cartilage was exposed through the original surgical incision. After gross observation, the cartilage articular surface including the lower end of the femur was cut, ELISA was used to detect noggin protein expression; Western blot was used to detect noggin protein expression in cartilage tissue, and statistical analysis of the data was performed. Results After 4 and 6 weeks of intervention, the expression of noggin protein in the Taohong Siwu Decoction group was lower than that in the model group and higher than that in the sham operation group (P<0.05). Compared with the glucosamine group, the expression of noggin protein in the Taohong Siwu Decoction group was lower, the difference was statistically significant (P<0.05). After 6 weeks of intervention, the articular surfaces of the model group, Taohong Siwu Decoction group and glucosamine group were all uneven, but the cartilage repair in the Taohong Siwu Decoction group and the glucosamine group was better than that in the model group. Conclusion Taohong Siwu Decoction can inhibit the expression of noggin protein and promote the repair of knee articular cartilage, and the effect of inhibiting the expression of noggin protein is better than that of glucosamine. |
| Key words: cartilage damage repair Taohong Siwu Decoction glucosamine noggin protein New Zealand rabbit |
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