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姚金龙,冯海波,李益亮,叶心雨,孙绍裘,刘晓岚.桃红四物汤对ANFH模型大鼠Notch1受体蛋白表达的影响——骨伤名师孙达武学术思想与临床经验研究（三）[J].湖南中医药大学学报英文版,2022,42(5):709-714.[Click to copy
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| 桃红四物汤对ANFH模型大鼠Notch1受体蛋白表达的影响——骨伤名师孙达武学术思想与临床经验研究（三） |
| 姚金龙,冯海波,李益亮,叶心雨,孙绍裘,刘晓岚 |
| (湖南中医药大学第二附属医院, 湖南 长沙 410005;湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 观察桃红四物汤对创伤性股骨头缺血性坏死(avascular necrosis of femoral head, ANFH)模型大鼠Notch1受体蛋白表达的影响。方法 从60只SD实验大鼠中随机挑选12只为假手术组，其余大鼠参照Norman方法进行造模构建ANFH模型，然后随机均分为桃红四物汤高剂量组、桃红四物汤中剂量组、桃红四物汤低剂量组、模型组，每组12只，对于假手术组，采用同样的术式显露髋关节，但是不进行造模而是直接缝合。待大鼠造模成功后，给予桃红四物汤高、中、低剂量组等量的高、中、低剂量桃红四物汤进行灌胃，模型组与假手术组分别予以等量的生理盐水进行灌胃。灌胃8周后处死各组大鼠，切取股骨头，采用Micro-CT观察各组大鼠股骨头形态变化，光镜下计算出股骨头平均空骨陷窝率，采用免疫组织化学法检测Notch1受体蛋白的表达水平。结果 通过Micro-CT观察，桃红四物汤各组较模型组股骨头软骨面损伤情况好，并且随着药物浓度的增加，桃红四物汤中、高剂量组较桃红四物汤低剂量组股骨头塌陷情况好。光学显微镜下观测，桃红四物汤各组大鼠平均空骨陷窝率均低于模型组（P<0.05或P<0.01），高于假手术组（P<0.05或P<0.01），而Notch1受体蛋白表达高于模型组和假手术组（P<0.05或P<0.01）；桃红四物汤低剂量组大鼠平均空骨陷窝率高于桃红四物汤高、中剂量组（P<0.05），而Notch1受体蛋白表达低于桃红四物汤中、高剂量组（P<0.05）。桃红四物汤高、中剂量组大鼠平均空骨陷窝率以及Notch1受体蛋白表达比较，差异无统计学意义（P>0.05）。结论 桃红四物汤能降低ANFH大鼠空骨陷窝率，治疗ANFH，并且该过程可能与桃红四物汤促进Notch1受体蛋白的表达有关。 |
| 关键词: 桃红四物汤 股骨头缺血性坏死 大鼠 空骨陷窝率 Notch1受体 |
| DOI：10.3969/j.issn.1674-070X.2022.05.003 |
| Received:November 12, 2021 |
| 基金项目:湖南省自然科学基金项目（2019JJ50458）；湖南省科技创新计划项目（2018SK51103）；湖南省中医骨伤临床医学研究中心项目（2020SK4013）；湖南省教育厅优秀青年项目（20B447）；湖南中医药大学校级科研联合项目（2020XJJJ054）。 |
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| Effect of Taohong Siwu Decoction on the expression of Notch1 receptor protein in ANFH model rats: Study on the academic thoughts and clinical experience of famous orthopaedic teacher Sun Dawu (3) |
| YAO Jinlong,FENG Haibo,LI Yiliang,YE Xinyu,SUN Shaoqiu,LIU Xiaolan |
| (The Second Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410005, China;Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To observe the effect of Taohong Siwu Decoction on the expression of Notch1 receptor protein in rats with avascular necrosis of femoral head (ANFH). Methods 12 SD rats were randomly selected from 60 SD rats as the sham operation group, and the other rats were modeled by Norman method to construct ANFH model, and then randomly divided into Taohong Siwu Decoction high-dose group, Taohong Siwu Decoction medium-dose group, Taohong Siwu Decoction low-dose group and model group, with 12 rats in each group. For the sham operation group, the hip joint was exposed using the same operation, but the hip joint was directly sutured without modeling. After successful modeling, the rats were given Taohong Siwu Decoction high-dose, medium-dose and low-dose groups of equal amount of high, medium and low dose Taohong Siwu Decoction for intragastric administration, model group and sham operation group were given equal amount of normal saline intragastric administration. After 8 weeks of intragastric administration, the rats in each group were sacrificed, and the femoral head was cut. Micro-CT was used to observe the morphological changes of the femoral head in each group, the average rate of hollow lacunae of the femoral head was calculated under light microscope, and the expression of Notch1 receptor protein was detected by immunohistochemistry. Results By Micro-CT observation, the femoral head cartilage damage was better in each group of Taohong Siwu Decoction than in model group, and with the increase of concentration, the femoral head collapse was better in Taohong Siwu Decoction medium-dose and high-dose groups than in Taohong Siwu Decoction low-dose group. Under a light microscope, the average rate of hollow lacunae of each group of Taohong Siwu Decoction was lower than that of model group (P<0.05 or P<0.01), and higher than that of sham operation group (P<0.05 or P<0.01), and the expression of Notch1 receptor protein was higher than that of model group and sham operation group (P<0.05 or P<0.01). The average rate of hollow lacunae in Taohong Siwu Decoction low-dose group was higher than that in Taohong Siwu Decoction high-dose and medium-dose groups (P<0.05), and the expression of Notch1 receptor protein was lower than that in Taohong Siwu Decoction medium-dose and high-dose groups (P<0.05). There were no significant differences in the average rate of hollow lacunae and Notch1 receptor protein expression between Taohong Siwu Decoction high-dose and medium-dose groups (P>0.05). Conclusion Taohong Siwu Decoction can reduce the rate of hollow lacunae in ANFH rats and treat ANFH, and this process may be related to Taohong Siwu Decoction promoting the expression of Notch1 receptor protein. |
| Key words: Taohong Siwu Decoction avascular necrosis of femoral head rats rate of hollow lacunae Notch1 receptor |
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