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孙涛,刘承鑫,李雅,郭志华,张彤瑜,魏佳明,易琼.益心泰方对慢性心力衰竭兔心肌组织β-MHC mRNA及蛋白表达的影响[J].湖南中医药大学学报英文版,2020,40(6):679-684.[Click to copy
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| 益心泰方对慢性心力衰竭兔心肌组织β-MHC mRNA及蛋白表达的影响 |
| 孙涛,刘承鑫,李雅,郭志华,张彤瑜,魏佳明,易琼 |
| (湖南中医药大学第一附属医院, 湖南 长沙 410007;湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 探讨益心泰方对慢性心力衰竭（chronic heart failure,CHF）兔模型心肌组织β-肌球蛋白重链(β-myosin heavy chain， β-MHC) mRNA及其蛋白表达的影响。方法 采用缩窄腹主动脉法+丙基硫氧嘧啶灌胃建立CHF兔模型。将成模家兔随机分成模型组、益心泰低剂量组、益心泰中剂量组、益心泰高剂量组及氯沙坦钾组，另设假手术组。每组分别予以相应药物灌胃，模型组及假手术组予以生理盐水灌胃，每日1次，连续灌胃4周。采用ELISA法检测心房钠尿肽(atrial natriuretic peptide,ANP)、脑钠肽（brain natriuretic peptide,BNP）水平；超声心动图仪检测心功能指标；采用Western blot法测检测心肌组织β-MHC蛋白含量；RT-PCR法测定β-MHC mRNA表达，并运用透射电镜观察心肌病理组织结构。结果 与假手术组比较，模型组ANP、BNP水平明显升高(P<0.01)；左心室射血分数(LVEF)、左心室短轴缩短率(LVFS)、E峰与A峰的比值(E/A)显著降低(P<0.01)；β-MHC蛋白及mRNA表达明显升高(P<0.01)；在电镜下观察，模型组心肌出现水肿、坏死等损伤表现。与模型组比较，各给药组ANP、BNP水平均显著下降(P<0.05)；各给药组LVEF、LVFS、E/A均显著升高(P<0.05)；各给药组β-MHC蛋白及β-MHC mRNA水平均显著降低(P<0.05)；在电镜下观察，各给药组心肌细胞损伤程度均得到改善。与益心泰低剂量组比较，益心泰中、高剂量组及氯沙坦钾组的上述检测指标均优于低剂量组(P<0.05)。结论 益心泰方可有效抑制心肌组织β-MHC蛋白及mRNA表达，降低ANP及BNP，改善心功能，从而发挥防治CHF的作用。 |
| 关键词: 益心泰方 慢性心力衰竭 β-MHC蛋白 BNP 心肌组织 |
| DOI：10.3969/j.issn.1674-070X.2020.06.007 |
| Received:January 06, 2020 |
| 基金项目:国家自然科学基金项目（81673955）；湖南省中医药科研计划项目（201833）；湖南中医药大学一流学科开放基金项目(2018ZYX43)。 |
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| Effects of Yixintai Fang on Expression of β-MHC mRNA and Its Protein in Myocardial Tissues of Rabbits with Chronic Heart Failure |
| SUN Tao,LIU Chengxin,LI Ya,GUO Zhihua,ZHANG Tongyu,WEI Jiaming,YI Qiong |
| (The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To explore the effects of Yixintai Fang on expression of β-myosin heavy chain (β-MHC) mRNA and its protein in myocardial tissues of rabbits with chronic heart failure (CHF). Methods Rabbit models of CHF were established by constricting abdominal aorta and giving propylthiouracil by gavage. Successfully modeled rabbits were randomly divided into a model group, a low-, middle-, and high-dose Yixintai Fang group and a losartan potassium group. Besides, a sham operation group was set up. The corresponding drug was given by gavage in each group. Normal saline was administered to rabbits of the model group and the sham operation group by gavage. The intervention lasted for 4 weeks, once per day. The levels of atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) were detected by ELISA. The cardiac function was detected by echocardiography. The content of β-MHC protein was detected by Western blot. The expression of β-MHC mRNA was detected by RT-PCR. The myocardial pathological structure was observed by transmission electron microscopy. Results Compared with the sham operation group, the ANP and BNP levels of the model group were significantly increased (P<0.01). The LVEF, LVFS, and E/A of the model group were significantly decreased (P<0.01). mRNA and protein expression levels of β-MHC in myocardial tissues were increased significantly (P<0.01). Under the observation of electron microscope, the myocardial tissue in the model group presented edema, necrosis and other injury manifestations. Compared with the model group, the levels of ANP and BNP of each administration group decreased significantly (P<0.05). LVEF, LVFS, and E/A of each administration group increased significantly (P<0.05). Moreover, mRNA and protein expression levels of β-MHC in each administration group were significantly decreased (P<0.05). Under the observation of electron microscope, the damage degree of cardiomyocytes in each group was improved compared with that in the model group. Compared with the low-dose Yixintai Fang group, the above indexes of Yixintai middle-, high-dose group and losartan potassium group were all better than the low-dose group (P<0.05). Conclusion Yixintai Fang can effectively inhibit the expression of mRNA and protein expression of β-MHC, reduce ANP and BNP, improve cardiac function, and thus play a role in the prevention and treatment of CHF. |
| Key words: Yixintai Fang chronic heart failure β-MHC protein BNP myocardial tissues |
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