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李银鑫,蒋鹏飞,曾志成,彭清华.青光安II号方有效组分对青光眼模型DBA/2J小鼠视网膜中RhoA、ROCK及Caspase-3蛋白表达的影响[J].湖南中医药大学学报英文版,2020,40(6):673-678.[Click to copy
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| 青光安II号方有效组分对青光眼模型DBA/2J小鼠视网膜中RhoA、ROCK及Caspase-3蛋白表达的影响 |
| 李银鑫,蒋鹏飞,曾志成,彭清华 |
| (湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 观察青光安Ⅱ号方有效组分对青光眼模型DBA/2J小鼠视网膜中RhoA、ROCK及Caspase-3蛋白表达的影响。方法 将8只（16只眼）雌性C57BL/6小鼠设为空白组（A组），48只（96只眼）雌性DBA/2J小鼠随机分成6组，每组8只（16只眼），分别为：模型组（B组）、益脉康分散片组（C组）、青光安Ⅱ号汤剂组（D组）、青光安Ⅱ号有效组分低浓度组（E组）、青光安Ⅱ号有效组分中浓度组（F组）、青光安Ⅱ号有效组分高浓度组(G组），B、C、D、E、F、G组DBA/2J小鼠喂养38周龄建立青光眼模型后开始干预，干预4周后Western blot法检测DBA/2J小鼠视网膜中RhoA、ROCK及Caspase-3蛋白的相对表达量。结果 干预4周后，与A组比较，B组RhoA、ROCK、Caspase-3蛋白相对表达量明显升高，差异均有统计学意义（P<0.05）；与B组比较，C、D、E、F、G组RhoA、ROCK、Caspase-3蛋白表达减少，差异均有统计学意义(P<0.05)；与C组比较，D、E、F组RhoA、ROCK、Caspase-3蛋白相对表达差异无统计学意义(P>0.05)；与C、D组比较，G组RhoA、ROCK、Caspase-3蛋白表达减少，差异有统计学意义(P<0.05)。结论 青光安Ⅱ号方有效组分能抑制Rho/ROCK信号通路及凋亡相关蛋白Caspase-3的表达，其中高浓度青光安Ⅱ号方有效组分效果优于益脉康分散片和青光安Ⅱ号方汤剂，推测青光安Ⅱ号方及其有效组分治疗青光眼的机制可能与其抑制Rho/ROCK信号通路及视网膜细胞凋亡相关蛋白表达有关。 |
| 关键词: 青光眼 青光安II号方 DBA/2J小鼠 Rho/ROCK信号通路 细胞凋亡 |
| DOI：10.3969/j.issn.1674-070X.2020.06.006 |
| Received:April 23, 2020 |
| 基金项目:国家自然科学基金项目（81874492，81904260）；湖南省自然科学基金项目（2018JJ3389）；湖南省研究生科研创新项目（CX2018B470，CX20190584）；中医药防治眼耳鼻咽喉疾病湖南省重点实验室（2017TP1018）；湖南中医药大学中医学国内一流建设学科资助项目；中央财政支持地方高校重点学科和中医眼科创新团队建设项目；国家中医药管理局重点学科中医眼科学建设项目；湖南省中医药防治眼耳鼻咽喉疾病与视功能保护工程技术研究中心建设项目。 |
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| Effects of the Effective Component of Qingguang'an II on the Expression of RhoA, ROCK and Caspase-3 Protein in the Retina of DBA/2J Mice with Glaucoma |
| LI Yinxin,JIANG Pengfei,ZENG Zhicheng,PENG Qinghua |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To observe the effect of the effective component of Qingguang'an Ⅱ on the expression of RhoA, ROCK and Caspase-3 protein in the retina of DBA/2J mice with glaucoma. Methods Eight female C57BL/6 mice (16 eyes) were set as the blank group (Group A), and 48 female DBA/2J mice (96 eyes) were randomly divided into 6 groups, with 8 rats (16 eyes) in each group. Theses groups were model group (group B), Yimaikang Dispersible Tablet group (group C), Qingguang'an Ⅱ Decoction group (group D), Qingguang'an Ⅱ effective component low concentration group (Group E), Qingguang'an Ⅱ effective component middle concentration group (Group F), Qingguang'an Ⅱ effective component high concentration group (Group G). Groups B, C, D, E, F and G were fed for 38 weeks after birth, and the glaucoma model was established. From week 39, all the groups were treated for 4 weeks, and the expression of RhoA, ROCK and Caspase-3 protein was detected by western blot. Results After 4 weeks’ intervention, compared with group A, the expression of RhoA, ROCK, and Caspase-3 protein in group B was significantly increased, and the difference was statistically significant (P<0.05); Compared with group B, the expression of RhoA, ROCK, and Caspase-3 protein in group C, D, E, F, G were decreased, and the difference was statistically significant (P<0.05); Compared with groups C, the expression of RhoA, ROCK, Caspase-3 protein in group D, E and F was not statistically significant different (P>0.05). Compared with group C, D, the expression of RhoA, ROCK, and Caspase-3 protein in group G was decreased, and the difference was statistically significant (P<0.05). Conclusion The effective component of Qingguang'an Ⅱ can inhibit the expression of Rho/ROCK signaling pathway and apoptosis-related protein Caspase-3 in retinal cells, and the effect of high concentration Qingguang'an Ⅱ effective component is better than Yimaikang Dispersible Tablets and Qingguang'an Ⅱ Decoction The mechanism of Qingguang'an Ⅱ and its effective component for glaucoma may be related to its inhibition of Rho/ROCK signaling pathway and the expression of apoptosis-related proteins in retinal cells. |
| Key words: Glaucoma Qingguang'an II prescription DBA/2J mice Rho/ROCK signaling pathway apoptosis |
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