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刘琼华,刘志生,郑煜煌,陈霞,贺波,郑力文.HIV/AIDS患者外周血中4种细胞因子IL-2、IL-4、IL-15和IL-21表达水平及其临床意义研究[J].湖南中医药大学学报英文版,2019,39(8):1011-1014.[Click to copy
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| HIV/AIDS患者外周血中4种细胞因子IL-2、IL-4、IL-15和IL-21表达水平及其临床意义研究 |
| 刘琼华,刘志生,郑煜煌,陈霞,贺波,郑力文 |
| (湖南娄底职业技术学院医学部, 湖南 娄底 417000;中南大学湘雅二医院感染科, 湖南 长沙 410011;中南大学湘雅医院检验科, 湖南 长沙 410008;中南大学湘雅二医院骨科, 湖南 长沙 410011) |
| 摘要: |
| 目的 观察外周血 4 种细胞因子（IL-2、IL-4、IL-15及IL-21）在HIV感染者/AIDS患者（HIV/AIDS）和正常人表达水平的区别及其与HIV RNA病毒载量和CD3+、CD4+细胞数量的相关性，分析其与HIV感染的关系和临床意义。方法 40例未经抗病毒治疗的HIV/AIDS 和31名正常人，采用ELISA法检测外周血4种细胞因子（IL-2、IL-4、IL-15及IL-21），使用流式细胞计数法检测外周血CD3+、CD4+ T 淋巴细胞数量，实时聚合酶链反应测定血清中HIV RNA水平。结果 未经抗病毒治疗的HIV/AIDS外周血IL-2、IL-15及IL-21水平低于正常人，而IL-4水平高于正常人（P<0.05）。CD3+、CD4+细胞数量和各细胞因子水平显示出一定的相关关系，IL-2和IL-15水平均与HIV-1 RNA载量呈负相关。结论 提示外周血4种细胞因子都可能参与了HIV感染的致病机制，并与HIV RNA病毒载量及CD3+、CD4+细胞数量相关。 |
| 关键词: 人免疫缺陷病毒 艾滋病 IL-2 IL-4 IL-15 IL-21 |
| DOI：10.3969/j.issn.1674-070X.2019.08.018 |
| Received:March 15, 2018 |
| 基金项目: |
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| Four Cytokine Expression Levels of IL-2, IL-4, IL-15 and IL-21 in Peripheral Blood of HIV/AIDS Patients and Their Clinical Significance |
| LIU Qionhua,LIU Zhisheng,ZHENG Yuhuang,CHEN Xia,HE Bo,ZHENG Liwen |
| (Department of Medicine, Loudi Medical School, Loudi, Hunan 420000, China;Department of Infectious Diseases, Second Xiangya Hospital, Central-South University, Changsha, Hunan 410011, China;Clinical Laboratory, Xiangya Hospital, Central-South University, Changsha, Hunan 410018, China;Department of Orthopedics, Second Xiangya Hospital, Central-South University, Changsha, Hunan 410011, China) |
| Abstract: |
| Objective To observe the differences of 4 cytokine levels between HIV/AIDS patients and normal persons, and their relevance with HIV RNA level and CD3+, CD4+ cell count, and to analyze the relationship with HIV infection and clinical significance. Methods A total of 40 HIV/AIDS patients without antiviral therapy and 31 normal persons were enrolled. Enzyme Linked Immunosorbent Assay (ELISA) was used to detect 4 cytokine levels (IL-2, IL-4, IL-15 and IL-21). At the same time, the number of CD3+ and CD4+ T lymphocytes in peripheral blood was detected by flow cytometry, and the level of HIV RNA in serum was determined by real-time polymerase chain reaction. Results IL-2, IL-15 and IL-21 in peripheral blood of HIV/AIDS patients without antiviral therapy were significantly lower than that of the normal people, and IL-4 level was higher than the normal people(P<0.05). The number of CD3+, CD4+ cells and the level of each cytokine showed a certain correlation, and IL-2 and IL-15 levels were negatively correlated with HIV-1 RNA load. Conclusion It is suggested that all 4 cytokines in peripheral blood may be involved in the pathogenesis of HIV infection and are related to HIV RNA viral load and the number of CD3+ and CD4+ cells. |
| Key words: human immunodeficiency virus AIDS IL-2 IL-4 IL-15 IL-21 |
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