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王艳,陈勋,李灿,娄婷,周日宝.基于响应面法优化白及原球茎液体培养基[J].湖南中医药大学学报英文版,2019,39(2):190-194.[Click to copy
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| 基于响应面法优化白及原球茎液体培养基 |
| 王艳,陈勋,李灿,娄婷,周日宝 |
| (湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 采用响应面优化（Box-Behnken design，BBD）法优选白及原球茎液体培养基，提高白及种子萌发形成原球茎的诱导率。方法 采用3水平4因素设计BBD实验，在30 g/L的蔗糖溶液中添加大量元素（MS）、6-苄氨基腺嘌呤（6-BA）、萘乙酸（NAA）和玉米素（ZT）作为白及原球茎液体培养基，并通过响应面法优选4种添加物浓度配比。结果 BBD实验模型设计成功可用于响应面结果优化，经响应面优化后，在1 mg/L MS、2 mg/L 6-BA、0.5 mg/L NAA、0.07 mg/L ZT条件下，白及原球茎诱导率可达（93.13±0.34）%，与预测值95.38%差异无统计学意义（P>0.05）。白及原球茎直播种苗存活率良好，长出的叶片数、植株高度、茎长及茎宽均超过种子直播种苗的数值。结论 在基本液体培养基中合理配比4种天然添加物可提高白及原球茎诱导率，进而提高种子萌发率。 |
| 关键词: 白及 原球茎 响应面 BBD设计 诱导率 |
| DOI：10.3969/j.issn.1674-070X.2019.02.009 |
| Received:August 10, 2018 |
| 基金项目:湖南省科技厅中央引导地方科技发展专项资金（2017XF5044）；湖湘中药资源保护与利用协同创新中心（湘教通〔2015〕351号）；2018年度湖南省大学生研究性学习和创新性实验计划项目立项（湘教通〔2018〕147号）；湖南中医药大学中药学一流学科项目（校行科字〔2018〕3号） |
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| Optimization of Bletilla striata Protocorm Liquid Medium Based on Response Surface Methodology |
| WANG Yan,CHEN Xun,LI Can,LOU Ting,ZHOU Ribao |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To increase the induction rate of Bletilla striata protocorms from seeds by optimizing Bletilla striata protocorm liquid medium based on Box-Behnken design (BBD) methodology. Methods The BBD experiment was designed with four factors and three levels. The Bletilla striata protocorm liquid medium was obtained by adding major elements (MS), 6-benzylamine adenine (6-BA), naphthylacetic acid (NAA), and zeatin (ZT) into 30 g/L sucrose solution. The concentrations of four additives (MS, 6-BA, NAA, and ZT) were optimized by response surface methodology (RSM). Results The successfully established BDD model could be used for optimization by RSM. After optimization by RSM, the induction rate of Bletilla striata protocorms could reached （93.13±0.34）% under the conditions of 1 mg/L MS, 2 mg/L 6-BA, 0.5 mg/L NAA, and 0.07 mg/L ZT; there was no significant difference between the experimental value and the predicted one (93.13% 0.34% vs 95.38%, P>0.05). The seedlings from direct seeding of Bletilla striata protocorms had a good survival rate, and were superior to those from direct seeding of Bletilla striata seeds in terms of the number of leaves, the height of the plant, and the length and width of the stem. Conclusion An appropriate composition of four natural additives in the basic liquid medium results in an increase in the induction rate of Bletilla striata protocorms and thus promotes the seed germination rate. |
| Key words: Bletilla striata protocorm response surface methodology Box-Behnken design induction rate |
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