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王孙亚,周兴,宾东华,韩忠,何清湖.右归丸及其拆方对肾阳虚大鼠血清T、LH和CORT的影响[J].湖南中医药大学学报英文版,2018,38(9):990-993.[Click to copy
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| 右归丸及其拆方对肾阳虚大鼠血清T、LH和CORT的影响 |
| 王孙亚,周兴,宾东华,韩忠,何清湖 |
| (湖南中医药大学, 湖南 长沙 410208;湖南中医药大学第一附属医院, 湖南 长沙 410007;岳阳市中医医院, 岳阳 414000) |
| 摘要: |
| 目的 探讨右归丸及其拆方对肾阳虚大鼠血清中睾酮（testosterone，T）、促黄体生成素（luteinizing hormone，LH）和皮质醇（cortisol，CORT）的影响。方法 取3月龄SPF级雄性SD大鼠50只，随机分成正常对照组、模型组、补阴药组、补阳药组、全方组，除正常对照组外，其他组均采用后肢肌内注射氢化可的松建立肾阳虚动物模型。造模成功后，补阴药组、补阳药组、全方组分别给予相应药物连续灌胃14 d，正常对照组与模型组给予等容量的生理盐水灌胃，停药后第2天，经麻醉后断头处死，心脏取血获得血清，摘除双侧睾丸。记录大鼠体质量及睾丸质量，并计算睾丸指数；用ELISA法测定血清中T、LH和CORT含量。结果 模型组大鼠较正常对照组大鼠体质量、睾丸指数及血清T、LH、CORT含量均降低（P<0.05）；治疗组中全方组较模型组大鼠大鼠体质量、睾丸指数及血清T、LH、CORT含量明显提升（P<0.05），补阴药组相对补阳药组增加大鼠体质量及血清LH含量（P<0.05），补阳药组相对补阴药提升睾丸指数及血清T含量作用较强（P<0.05）；补阴组和补阳组对血清CORT含量均无明显作用（P>0.05）。结论 右归丸全方可对肾阳虚证大鼠体质量、睾丸指数、血清中的T、LH和CORT表达水平产生良性影响，右归丸方中的补阴药在提升肾阳虚大鼠体质量和增加血清中LH的含量方面调控作用较好，补阳药在增加血清中T的含量和提高睾丸指数方面调控作用较好，补阴药和补阳药对血清中CORT表达水平的调控均无明显作用。 |
| 关键词: 右归丸 拆方 阴中求阳 肾阳虚 睾酮 促黄体生成素 皮质醇 |
| DOI：10.3969/j.issn.1674-070X.2018.09.005 |
| Received:September 18, 2017 |
| 基金项目:国家自然科学基金（81673984）；湖南省教育厅优秀青年项目（16B200）；湖南中医药大学研究生创新项目（1023-0002001032）；湖南中医药大学"一方研究生科研创新项目"（YF201701）。 |
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| Effects of Yougui Pill and Its Components on Serum T, LH, and CORT in Rats with Kidney Yang Deficiency |
| WANG Sunya,ZHOU Xing,BIN Donghua,HAN Zhong,HE Qinghu |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The First Affiliated Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;Yueyang Hospital of Traditional Chinese Medicine, Yueyang, Hunan 414000, China) |
| Abstract: |
| Objective To explore the effect of Yougui Pill and its components on serum testosterone (T), luteinizing hormone (LH), and cortisol (CORT) in rats with kidney Yang deficiency. Methods A total of 50 3-month-old specific pathogen-free male Sprague-Dawley rats were randomly divided into normal control group, model group, Yin nourishing component group, Yang tonifying component group, and whole prescription group. All rats except those in the normal control group were intramuscularly injected with hydrocortisone in the hind legs to establish an animal model of kidney Yang deficiency. After completion of model establishment, the rats were treated for 14 consecutive days as follows:intragastric administration of corresponding medicines for the Yin nourishing component group, the Yang tonifying component group, and the whole prescription group; intragastric administration of an equal amount of normal saline for the normal control group and the model group. On the second day of medicine discontinuation, the rats were anesthetized and decapitated; serum was collected by cardiac puncture, and both testes were also collected. Body weight and testis weight of the rats were recorded, and testicular index was calculated. ELISA was used to determine the levels of T, LH, and CORT. Results Compared with the normal control group, the model group had significantly reduced body weight, testicular index, and serum levels of T, LH, and CORT (P<0.05). The whole prescription group had significantly increased body weight, testicular index, and serum levels of T, LH, and CORT compared with the model group (P<0.05). Compared with the Yang tonifying component group, the Yin nourishing component group had significantly greater increases in body weight and serum LH level (P<0.05), but significantly less increases in testicular index and serum T level (P<0.05). Neither of the above two groups showed a significant change in serum CORT level (P>0.05). Conclusion The whole prescription of Yougui Pill has favorable effects on body weight, testicular index, and serum levels of T, LH, and CORT in rats with kidney Yang deficiency; Yin nourishing component in the prescription reveals good effects in increasing body weight and serum LH level, and Yang tonifying component reveals good effects in increasing testicular index and serum T level; neither of the above components has an obvious effect in regulating the level of serum CORT. |
| Key words: Yougui Pill seperated prescription reinforcing Yang from Yin kidney Yang deficiency testosterone luteinizing hormone cortisol |
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