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魏歆然,郑雪娜,裴芸,吴雪芬,刘丽,陈小丽,文琪琦,岳增辉,魏高文.大鼠失眠模型造模中对氯苯丙氨酸混悬液改良法的探讨[J].湖南中医药大学学报英文版,2018,38(9):986-989.[Click to copy
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| 大鼠失眠模型造模中对氯苯丙氨酸混悬液改良法的探讨 |
| 魏歆然,郑雪娜,裴芸,吴雪芬,刘丽,陈小丽,文琪琦,岳增辉,魏高文 |
| (湖南中医药大学第一附属医院, 湖南 长沙 410007;湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 探讨大鼠失眠模型造模中对氯苯丙氨酸（para-chlorophenylalanine，PCPA）混悬液配制的改良方法，并对造模效果进行评价。方法 98只SD大鼠中50只用NaOH溶液配制法、24只用NaHCO3溶液配制法和24只用改良法进行失眠大鼠造模，探讨制作PCPA致失眠模型的最佳方法。结果 NaOH溶液配制法50只大鼠造模失败，NaHCO3溶液配制法24只大鼠造模失败，改良法24只大鼠造模成功，且PCPA剂量以0.5 g/kg造模效果最好（P<0.05）。结论 改良的PCPA混悬液的配制法可以成功制作大鼠失眠模型。 |
| 关键词: 对氯苯丙氨酸 失眠 造模 |
| DOI：10.3969/j.issn.1674-070X.2018.09.004 |
| Received:March 30, 2017 |
| 基金项目:国家重点基础研究发展计划（973计划）（2014CB543102）。 |
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| An Improved Method for the Preparation of Para-chlorobenzalanine Suspension in a Rat Model of Insomnia |
| WEI Xinran,ZHENG Xuena,PEI Yun,WU Xuefen,LIU Li,CHEN Xiaoli,WEN Qiqi,YUE Zenghui,WEI Gaowen |
| (The First Affiliated Hospital of Hunan Universty of Chinese Medicine, Changsha, Hunan 410007, China;Hunan College of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To explore the improved method of preparing para-chlorophenylalanine (PCPA) suspension in a rat model of insomnia, and evaluate the effect of modeling. Methods In the 98 SD rats, 50 rats were treated with NaOH solution preparing method, 24 SD rats were modeled with NaHCO3 solution preparing method, and 24 SD rats were treated with the improved method to establish the model of insomnia rats. Results There were 50 failed model rats with NaOH solution preparing method, 24 failed model rats with NaHCO3 solution preparing method. The 24 model rats succeed by the improved method, and the 0.5 g/kg of PCPA was the best dose to establish the model (P<0.05). Conclusion The improved preparing method of PCPA suspension can successfully make the rat model of insomnia. |
| Key words: para-chlorophenylalanine insomnia model |
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