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李云耀,陈林,孟英才,谭洋,肖水平,易刚强,裴刚.超临界CO2萃取法与水蒸气蒸馏法提取黄连木嫩叶挥发油及GC-MS分析[J].湖南中医药大学学报英文版,2016,36(3):24-26,46.[Click to copy
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| 超临界CO2萃取法与水蒸气蒸馏法提取黄连木嫩叶挥发油及GC-MS分析 |
| 李云耀,陈林,孟英才,谭洋,肖水平,易刚强,裴刚 |
| (湖南中医药大学药学院, 湖南 长沙 410208) |
| 摘要: |
| 目的 对超临界CO2流体萃取法和水蒸气蒸馏法提取黄连木嫩叶挥发油的化学成分进行比较分析。方法 用超临界CO2流体萃取法和水蒸气蒸馏法提取黄连木嫩叶挥发油，对其化学成分采用GC-MS分析。结果 超临界CO2流体萃取法与水蒸气蒸馏法得到的挥发油成分具有一定差异，分别鉴定出18种和24种化学组分。其中8种为相同的主要挥发油成分，如β-月桂烯、D-柠檬烯、1-碘代-十六烷等。结论 两种方法提取的黄连木嫩叶挥发油成分种类存在一定差异，但是主要挥发油成分的提取效果相当，综合考虑需要组分可以选择合适的提取方法。 |
| 关键词: 黄连木 超临界CO2流体萃取法 水蒸气蒸馏法 挥发油 GC-MS法 |
| DOI：10.3969/j.issn.1674-070X.2016.03.007 |
| Received:September 30, 2015 |
| 基金项目:2011年湖南省大学生研究性学习和创新性实验计划项目；湖南省科技厅科研项目（2012FJ4286）。 |
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| GC-MS Analysis of Volatile Oil Extracted from Tender Leaf of Pistacia chinensis Bunge with Supercritical CO2 Extraction and Steam Distillation |
| LI Yunyao,CHEN Lin,MENG Yingcai,TAN Yang,YI Gangqiang,PEI Gang |
| (School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To make a comparative analysis of volatile oil constituents extracted from tender leaf of Pistacia chinensis Bunge with supercritical CO2 extraction and steam distillation. Methods The supercritical CO2 extraction and steam distillation methods were used to extract the volatile oil from tender leaf of Pistacia chinensis Bunge and their constituents were determined by GC-MS. Results The constituents extracted by supercritical CO2 extracts and steam distillation showed obvious difference, 18 and 24 kinds of volatile oil were determined, repectively. The result concluded 8 kinds of main volatile oil, such as β-myrcene, D-Limonene, 1-Iodohexadecane. Conclusion The methods showed similar results on extrating of main constutents from tender leaf of Pistacia chinensis Bunge, but also existting some differences on the kinds of volatile oil. So we can take the best extraction method into consideration obeying our needs. |
| Key words: pistacia supercritical CO2 extraction steam distillation volatile oil GC-MS method |
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