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任婷； 黄政德； 田雪飞； 陈楚淘；.心痛舒含药血清预处理对乳鼠心肌细胞缺氧/复氧损伤及TNF-α、IL-1β的影响[J].湖南中医药大学学报英文版,2012,32(11):11-15.[Click to copy
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| This paper
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| 心痛舒含药血清预处理对乳鼠心肌细胞缺氧/复氧损伤及TNF-α、IL-1β的影响 |
| 任婷；黄政德；田雪飞；陈楚淘； |
| (湖南中医药大学医学院; 湖南中医药大学第一中医临床学院; 湖南中医药大学中西医结合学院; 湖南中医药大学研究生处) |
| 摘要: |
| 目的观察心痛舒含药血清预处理对缺氧/复氧乳鼠心肌细胞核因子-κBp65(NF-κBp65)及其下游调控因子肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)的影响,探讨心痛舒对心肌细胞缺氧/复氧损伤的保护作用机制。方法原代培养SD乳鼠心肌细胞,建立模拟心肌细胞缺氧/复氧损伤模型,设正常组、模型组、阳性对照组、心痛舒含药血清预处理组共4组。罗丹明B染色法观察心肌细胞形态,ELISA法测定培养液上清TNF-α、IL-1β含量,半定量RT-PCR方法检测NF-κBp65 mRNA表达水平。结果与模型组比较,阳性对照组、心痛舒含药血清预处理组TNF-α、IL-1β水平降低,NF-κBp65 mRNA的表达水平下降,差异有统计学意义(P<0.01);与阳性对照组比较心痛舒含药血清预处理组NF-κBp65 mRNA表达降低,差异有统计学意义(P<0.05)。结论心痛舒含药血清预处理可通过抑制缺氧-复氧心肌细胞炎症反应途径发挥保护作用,其机制与抑制NF-κB活化,从而抑制下游炎性细胞合成有关。 更多还原 |
| 关键词: 心痛舒 心肌细胞 炎症因子 心肌细胞核因子-κBp65 肿瘤坏死因子-α 白介素-1β 乳鼠 作用机制 |
| DOI： |
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| 基金项目:国家自然科学基金资助项目(30973750);湖南省教育厅重点科研项目(09A071);湖南省重点学科中医内科学资助项目 |
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| Effects of preconditioning with serum containing Xintongshu on the newborn rat's myocardial cells with hypoxia and reoxygenration injury and the expression of TNF-α、IL-1β |
| REN Ting1,HUANG Zheng-de2,TIAN Xue-fei3,CHEN Chu-tao4 |
| (1.Medical College,TCM University of Hunan,Changsha,Hunan 410208,China; 2.First TCM Clinical College,TCM University of Hunan,Changsha,Hunan 410007,China; 3.College of Integrated Chinese and Western Medicine,TCM University of Hunan,Changsha,Hunan 410208,China; 4.Postgraduate Department,TCM University of Hunan) |
| Abstract: |
| Objective To observe the regulating effects of preconditioning with serum containing Xintongshu on the expression of nuclear factor-κBp65(NF-κBp65), tumor necrosis on the hypoxia and reoxygen injury myocardiL cell model of new born rat. Methods The myocardial cells were isolated from neonatal rats and cultured, then modelled eith hypoxia and reoxygen injury. The modeled myocardial cells were randomly divided into four groups, incliding normal control group,positive control group and preconditioning group treated with serum containing Xintongshu group(Xintongshu group). The myocardial cells morphologic changes wre obseved by Rhodamine B stain.The TNF-αand IL-1β proteins in culture media wre detected by ELISA and thw mRNA expression levels of NF-κBp65 was deteced by RT-PCR.Results Compared with modelcontrol group, the expression level of TNF-αand IL-1βproteins and NF-κBp65 mRNA in control group and Xintongshu group were decreased. There were statistical differences(P<0.01).Compared withpositive control group , the expression levels of NF-κBp65 mRNA in Xintongshu group were statistically decreased (P<0.05). Conclusion The preconditioning with serum containing Xintongshu can exert a protective effect by inhibiting the inflammatory reaction pathway of the hypoxia and reoxygenration myocardial cells. The effect may be related to the decrease of the NF-κB p65 mRNA expression level and further inhibition of thegeneration of inflammatory cells. |
| Key words: Xintongshu myocardial cell inflammatory cytokined NF-κB p65 TNF-α IL-1β new born rat mechanism of iactiom |
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