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黄小平1，谭华1，刘文龙2，邓常清1*.黄芪总苷和三七总皂苷配伍对小鼠脑缺血再灌注脑组织能量障碍的影响[J].湖南中医药大学学报英文版,2012,32(7):19-22.[Click to copy
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| 黄芪总苷和三七总皂苷配伍对小鼠脑缺血再灌注脑组织能量障碍的影响 |
| 黄小平1，谭华1，刘文龙2，邓常清1* |
| (1.湖南中医药大学医学院2.湖南中医药大学药学院) |
| 摘要: |
| 目的探讨黄芪总苷（AST）和三七总皂苷（PNS）对脑缺血再灌注小鼠能量代谢障碍的影响。方法C57BL/
6 小鼠被随机分成假手术组、模型组、AST 组（110 mg/(kg·d)，ig），PNS 组（115 mg/(kg·d)，ig），AST+PNS 配伍组（AST
110 mg/(kg·d)+PNS 115 mg/(kg·d)，ig）和依达拉奉组（8 mg/(kg·d)，ip），分别给药，连用4 d。在最后1 次给药1 h 后
制作脑缺血再灌注损伤模型，检测脑组织三磷酸腺苷（ATP）、二磷酸腺苷（ADP）、一磷酸腺苷（AMP）含量和Na+-K+-
ATPase 酶活性。结果AST 和PNS 配伍对脑缺血再灌注后ATP、ADP 含量、总腺苷酸池（TAN）值和ATP 酶活性的降
低具有协同抑制作用，差异具有统计学意义（P<0.01），对能荷（EC）值的减少具有相加的抑制作用（P<0.05）。结论
AST 和PNS 配伍可以明显改善脑缺血在灌注后能量代谢障碍， 其作用机制可能与AST 和PNS 能协同提高脑组织能
量的利用和生成，改善能荷、增强Na+-K+-ATPase 的活性有关。 |
| 关键词: 黄芪总苷 三七总皂苷 配伍 脑缺血再灌注 能量代谢障碍 小鼠 |
| DOI： |
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| 基金项目:湖南省教育厅重点资助项目（08A050）。 |
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| Effects of Astragalosides and Panax notoginsengsaponins combination on energy metabolism of brain tissuesafter cerebral ischemia-reperfusion in mice |
| HUANG Xiao-ping1， TAN Hua1， LIU Wen-long2， DENG Chang-qing1 |
| (1. Medical College， TCM University of Hunan2. Pharmaceutical College， TCM University of Hunan) |
| Abstract: |
| Objective To investigate the effects of Astragalosides(AST) and Panax notoginseng
saponins (PNS) combination on energy metabolism disturbance after cerebral ischemia -reperfusion
in mice. Methods C57BL/6 mice were randomly divided into 6 groups as follows： sham-operated
group，model group，Astragalosides group(AST，110 mg/kg·d，ig)，Panax notoginseng saponins group
(PNS，115 mg/kg·d，ig) ，AST combined with PNS group (AST 110 mg/kg·d+PNS 115 mg/kg·d，ig)
and 3-methyl-1-phenyl-2-pyrazoline-5-one group (edaravone，8 mg/kg·d，ip). All mice were administered
respectively and lasted for 4 d. After the last treatment for 1 h，cerebral ischemia-reperfusion
injury model was established. Then, the contents of adenylate(ATP，ADP，AMP) in brain tissues
were measured and the activities of Na+-K+-ATPase were evaluated. Results AST combined with
PNS exhibited synergistic inhibition effects on the decrease of ATP and ADP contents, total ade-nine nucleotides (TAN) and ATPase activities (all P<0.01)，and exhibited addition inhibition effects
on the decrease of energy charge (EC) (P <0.05). Conclusion These results suggest that
AST combined with PNS significantly improve cerebral energy metabolism dysfunction after ischemia-
reperfusion. The underlying mechanism may be that AST and PNS synergistically promote
the supply and generation of energy， improve the energy charge， enhance Na+-K+-ATPase activity
in brain tissues. |
| Key words: Astragalosides Panax notoginseng saponins combination cerebral ischemiareperfusion energy metabolism dysfunction mouse |
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