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黄献平，郑景辉，袁肇凯，李勇华，王丽萍，简维雄，孙贵香，.骨髓间充质干细胞移植大鼠缺血心肌后GATA-4 mRNA的表达[J].湖南中医药大学学报英文版,2011,31(9):12-15.[Click to copy
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| 骨髓间充质干细胞移植大鼠缺血心肌后GATA-4 mRNA的表达 |
| 黄献平，郑景辉，袁肇凯，李勇华，王丽萍，简维雄，孙贵香， |
| (湖南中医药大学中医诊断学国家重点学科国家中医药管理局病理生理实验室 湖南省高校重点实验室) |
| 摘要: |
| 目的观察骨髓间充质干细胞(MSCs)移植于大鼠缺血心肌后，心脏早发育基因DATA-4 mRNA的表
达、方法设模型组、假手术组和空白对照组，其中模型组采用“冠脉结扎法”建立大鼠急性心肌梗死(AMI)模型、收
集从SD雄性大鼠胫骨和股骨骨髓中分离、培养、鉴定后的MSCs，密度调至3x10`/100 },L悬液，移植于缺血心肌边
缘组织的3个位点直接注入共100 },L的MSCs细胞悬液，假手术组和空白对照组均在与模型组相同注射部位的3
个位点直接注入共100 },L的MSCs细胞悬液，28 d后处死大鼠进行相关实验样木处理〔采用逆转录聚合酶链反应
(RT-PCR)技术检测心肌组织中DATA-4 mRNA的表达、结果MSCs移植模型组和假手术组的大鼠心肌中存在
DATA-4 mRNA的表达;而空白组的心肌中未检出DATA-4 mRNA的表达，对其IOD值半定量分析，模型组高于假
手术组和空白组，差异有统计学意义(P<0.05或<0.01)、结论MSCs在进入缺血心肌环境后，细胞内的心肌特异转录
过程被再次激活.确立了MSCs向心肌细胞分化的方向、 |
| 关键词: 心肌缺血 骨髓间充质干细胞 心肌梗死 DATA-4 大鼠 |
| DOI： |
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| 基金项目:l国家自然科学基金资助项日(30772696,30973717);湖南省研究生创新基金(CX2009B168) |
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| Expression of GATA-4 mRNA in transplanted MSCs ischemic myocardium in rats |
| HUAN Xian-ping, ZHENG Jing-hui, YUAN Zhao-kai, LI Yong-hua, WANG Li-ping,
JIAN Wei-xiong, SUN Gui-xiang |
| ((State I}ey Subject of Diagnostics, TCM University
State Administration of TCM, Changs
of Hunan, Pathophysiological Laboratory of
ha,) |
| Abstract: |
| (Abstract〕 Objective To observe the expression of genes GATA-4 mRNA in the ear-
1y heart development after bone marrow mesenchymal stem cells(MSCs) were transplanted into is-
chemic myocardium. Methods The model group, sham group and control group were set, and the
acute myocardial infarction (AMI) model was established according[()"coronary occlu-
sign" method., MSCs were collected the isolated, cultured and identified marrow from the tibia
and femur of SD male rats, the density of MSCs was adjusted[()3x106/100 N.,L and then[()be
transplanted into three points of ischemic myocardium edge in model group and be injected 100
N.,L, and in sham group and control group were injected 100 N.,L of MSCs suspension into the
same places. The rats were killed after 28 days[()get the test samples, and then the myocardial
expression of GATA -4 mRNA was detected wit reverse transcription polymerase chain reaction (RT -PCR).
but did not
expressions
sion After
Results The GATA -4 mRNA expression existed in model group and shaml group;
in
7n
control group, while the semi-quantitative analysis of IOD values
model group
、、ere
higher than those in other two groups(P<
MSCs
goes into the
was activated
environment of ischemic myocardium,
0.01
[卜e
()r
showed
P<0.05).
that the
Conclu-
transcriptron
cardiac-specific
myocardial cells.
()n fle
again, which established the differentiation direction
process
of MSCS
()f[() |
| Key words: GATA-4 words口myocardial ischemia bone
marroh
mesenchymal
stem
cells myocardial infarction rats |
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