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谭舒怀,刘迈兰,曾理,刘红华,刘琼,罗坚,常小荣,葛君芸.隔药饼灸对动脉粥样硬化兔血管内皮修复与主动脉中Notch1及Jagged1蛋白表达的影响[J].湖南中医药大学学报,2021,41(10):1482-1486[点击复制] |
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| 隔药饼灸对动脉粥样硬化兔血管内皮修复与主动脉中Notch1及Jagged1蛋白表达的影响 |
| 谭舒怀,刘迈兰,曾理,刘红华,刘琼,罗坚,常小荣,葛君芸 |
| (湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 观察隔药饼灸对动脉粥样硬化兔受损血管内皮结构的修复以及对Notch1、Jagged1蛋白表达的影响。方法 将48只兔按随机数字表随机分为6组，即正常组、模型组、隔药饼灸组、直接灸组、药饼敷贴组、阿托伐他汀钙组，每组8只。正常组兔采用普通饲料喂养，其余各组兔采取高脂饲养法喂养8周，制备动脉粥样硬化兔模型。待造模成功后，除正常组、模型组外，隔药饼灸组选取两组穴位（“巨阙”“天枢”“丰隆”穴；“心俞”“肝俞”“脾俞”穴）隔日交替施灸，每穴灸4壮（约30 min）；药饼敷贴组选穴与隔药饼灸组相同，每组穴隔日交替敷贴药饼；阿托伐他汀钙组每天将阿托伐他汀钙片（1.96 mg·kg-1·d-1）碾成粉末后拌入第1餐饲料中喂食。均每日1次，连续8周。治疗后，HE染色观察主动脉血管壁形态结构，免疫组化法测定胸主动脉Notch1、Jagged1蛋白表达情况。结果 与正常组相比，模型组主动脉壁结构受损明显；与模型组相比，隔药饼灸组内皮受损结构有显著好转，直接灸组、阿托伐他汀钙组、药饼敷贴组主动脉内皮病理变化也有所减轻。与正常组比较，模型组主动脉Notch1、Jagged1蛋白表达差异无统计学意义（P>0.05）；干预后，与模型组相比，隔药饼灸组、阿托伐他汀钙组、直接灸组主动脉Notch1、Jagged1蛋白表达均有不同程度的升高（P<0.01或P<0.05），药饼敷贴组主动脉Notch1、Jagged1蛋白表达差异无统计学意义（P>0.05）；与隔药饼灸组相比，直接灸组、药饼敷贴组主动脉Notch1、Jagged1蛋白表达均有不同程度的降低（P<0.01或P<0.05），阿托伐他汀钙组主动脉Notch1、Jagged1蛋白表达差异均无统计学意义（P>0.05）。结论 隔药饼灸能促进主动脉中Notch1与Jagged1蛋白的表达，修复受损血管内皮结构。 |
| 关键词: 隔药饼灸 动脉粥样硬化 血管内皮修复 Notch1 Jagged1 |
| DOI：10.3969/j.issn.1674-070X.2021.10.002 |
| 投稿时间：2021-04-14 |
| 基金项目:国家重点基础研究发展计划（973计划）项目（2015CB554502）；国家自然科学基金面上项目（82074559，81874430）；湖南省自然科学基金项目（2020JJ5414）；长沙市杰出创新青年培养计划项目（kq1905036）；湖南省教育厅优秀青年基金项目（19B435，19B428）；湖南中医药大学中医学一流学科开放基金项目（2018ZYX31）；湖南中医药大学第一附属医院湖南省院士专家工作站（石学敏）开放基金（2019YSZJJ）；湖南省中医药科研计划课题项目（201964）；国家自然科学基金项目（81874509）；国家中医药管理局全国名老中医药专家传承工作室项目（国中医药人教函〔2019〕41号）；常小荣国家级教学名师工作室建设项目。。 |
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| Effect of Herbal Cake-separated Moxibustion on the Expression of Notch1 and Jagged1 Protein in Vascular Endothelial Repair and Aorta of Arteriosclerosis Rabbits |
| TAN Shuhuai,LIU Mailan,ZENG Li,LIU Honghua,LIU Qiong,LUO Jian,CHANG Xiaorong,GE Junyun |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To observe the effect of cake-separated moxibustion on the repair of damaged vascular endothelial structure and the expression of Notch1 and Jagged1 in arteriosclerosis rabbits. Methods 48 rabbits were randomly divided into 6 groups:normal group, model group, cake-separated moxibustion group, direct moxibustion group, medicine cake application group and atorvastatin calcium group, with 8 rabbits in each group. The rabbits in normal group were fed with normal diet, and the rabbits in other groups were fed with high fat diet for 8 weeks to establish arteriosclerosis rabbit model. After the modeling was successful, in addition to the normal group and the model group, two groups of acupuncture points were selected for the cake-separated moxibustion group["Juque" (RN14), "Tianshu" (ST25), "Fenglong" (ST40); "Xinshu" (BL15), "Ganshu" (BL18) and "Pishu" (BL20)]. Each group was treated with alternate moxibustion every other day for 4 moxa sticks (about 30 minutes). The acupoint selection of the medicine cake application group was the same as that of the cake-separated moxibustion group. Each group was treated with alternate moxibustion every other day. In the atorvastatin calcium group, the atorvastatin calcium tablets (1.96 mg·kg-1·d-1) were ground into powder and fed into the first diet once a day for 8 weeks. After treatment, HE staining was used to observe the morphological structure of aortic vascular wall, and the expression of Notch1 and Jagged1 protein in thoracic aorta was detected by immunohistochemistry. Results Compared with the normal group, the structure of aorta wall in the model group was damaged obviously; compared with the model group, the structure of endothelium in the cake-separated moxibustion group was improved significantly, the pathological changes of aortic endothelium in direct moxibustion group, atorvastatin calcium group and medicine cake application group were also reduced. Compared with the normal group, the expression of Notch1 and Jagged1 protein in the model group had no significant difference (P>0.05). After intervention, compared with the model group, the expression levels of Notch1 and Jagged1 protein in aorta of cake-separated moxibustion group, atorvastatin calcium group and direct moxibustion group were increased in different degree (P<0.01 or P<0.05), but Notch1 and Jagged1 protein expression had no significant difference in medicine cake application group (P>0.05). The expression levels of Notch1 and Jagged1 protein in aorta of direct moxibustion group and medicine cake application group were decreased to different degrees compared with that of cake-separated moxibustion group (P<0.01 or P<0.05). There was no significant difference in expression of Notch1 and Jagged1 protein in aorta of atorvastatin calcium group (P>0.05). Conclusion Cake-separated moxibustion can promote the expression of Notch1 and Jagged1 protein in aorta and repair the damaged vascular endothelium. |
| Key words: cake-separated moxibustion arteriosclerosis vascular endothelial repair Notch1 Jagged1 |
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