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唐金凤； 鲁耀邦； 桂柳姿；.黄花石蒜花蕊和花葶组织中总RNA提取方法的比较[J].湖南中医药大学学报,2012,32(1):31-34[点击复制] |
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| 黄花石蒜花蕊和花葶组织中总RNA提取方法的比较 |
| 唐金凤；鲁耀邦；桂柳姿； |
| (湖南中医药大学药学院中药药性与药效三级科研实验室湖南省中药现代化研究实验室) |
| 摘要: |
| 目的筛选适合黄花石蒜花蕊和花葶组织中总脱氧核糖核苷酸(RNA)的提取方法。方法采用CTAB法、SDS法和植物总RNA提取试剂盒法对黄花石蒜花蕊和花葶组织中总RNA进行提取并比较其效果。结果 CTAB法和SDS法均不能提取到完整的RNA;植物总RNA提取试剂盒能有效地去除蛋白质、多糖及DNA,28S条带的荧光亮度是18S条带的2倍左右,OD260/280值在1.8~2.0之间,花蕊和花葶的产率分别为304.1μg/g和255.8μg/g,并且试剂盒法提取的总RNA通过RT-PCR扩增能获得特异性条带。结论试剂盒法适合黄花石蒜花蕊和花葶组织总RNA的提取,提取的总RNA质量高、完整性好、产率高,符合后续实验的要求。 |
| 关键词: 黄花石蒜 花蕊 花葶 脱氧核糖核苷酸 |
| DOI： |
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| 基金项目:湖南省自然基金资助项目(10JJ2024);湖南省教育厅科研基金资助项目(07A052) |
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| Comparison of total RNA extractionbetween Lycorisaurea Herb Flowers and stapes |
| TANG Jin-feng, LU Yao-bang*，GUI Liu-zi |
| (Pharmaceutical College,TCM University of Hunan) |
| Abstract: |
| (Abstract〕 Objective To screen a suitable method for extracting total RNA from Lycoris au-
rea Herb flowers and stapes. Methods The CTAB and SDS extracting techniques and plant total
RNA extraction were used to extract the total RNA from Lycoris aurea Herb flowers and stapes
and the techniques were compared. Results Both CTAB and SDS methods were not effective to
extract intergative total RNA; the plant total RNA extraction kit could effectively remove protein,
polysaccharides and DNA, 28S bands of fluorescence intensity was about 2 times of the 18S bands,
the values of ODzboizso was between 1.8 and 2.0, the draba of flowers and flower yield were
304.1 p,到g and 255.8 N.,g/g, and the total RNA extracted by kit extraction amplificated by RT-
PCR could gain the specific band. Conclusion The plant total RNA extraction kit is more suitable
to extract total RNA from Lycoris aurea Herb flowers and stapes, and the total RNA is much su-
perior qualitive and better integritive. |
| Key words: Lycoris
aurea
Herb stamens stapes RNA kit method |
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