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伍茶花;丁扬洲;裴刚;韦熹苑;廖新华;田雪飞;.不同菱角壳提取物抑制肺癌A549细胞生长的研究[J].湖南中医药大学学报,2012,32(1):27-30[点击复制] |
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| 不同菱角壳提取物抑制肺癌A549细胞生长的研究 |
| 伍茶花;丁扬洲;裴刚;韦熹苑;廖新华;田雪飞; |
| (湖南中医药大学药学院) |
| 摘要: |
| 目的研究不同菱壳水和乙醇提取物的抗癌活性。方法采用Folin比色法测定不同品种菱壳提取物中多酚的含量,通过MTT法测定二角菱壳、四角菱壳的水提取物、醇提取物对肺癌A549细胞生长抑制作用。结果四角菱壳多酚含量高于二角菱壳,醇提物多酚含量高于水提物;二角菱壳醇提取物对肺癌A549细胞的抑制率高于水提物,差异具有统计学意义(P<0.01);四角菱壳水提物对抑制率高于二角菱壳水提物肺癌A549细胞的生长,差异具有统计学意义(P<0.01)。结论菱角壳提取物对肺癌A549细胞生长具有较强的抑制作用,且菱角壳的醇提取物优于水提取物,四菱角壳优于二角菱壳。 |
| 关键词: 二角菱壳 四角菱壳 A549细胞 肺癌 Folin比色法 四甲基偶氟唑盐法 |
| DOI： |
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| 基金项目:湖南省高校“中药新药创制与资源综合持续利用”科技创新团队资助 |
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| Study of different extracts from water caltrop shellson inhibiting lung cancer cell line A549 |
| WU Cha-hua, DING Yang-Zhou, PEI Gang*, WEI Xi-yuan, LIAO Xi-hua, TIAN Xue-fei |
| (TCM university of Hunan, Changsha) |
| Abstract: |
| (Abstract〕 Objective To investigate the inhibiting effect of various water and alcohol extracts
from different kinds of water caltrop shells on the proliferating activity of lung cancer cell line
A549. Methods Polyphenol content of water and ethanol extracts from different kinds of wa-
ter caltrop were determined with Folin assay, the inhibiting effects of water and alcohol extracts
from the two angle and corners caltrop shells on lung cancer cell line A549 was determined with
MTT assay. Results The polyphenol contents in corners caltrop shells was higher than that in two
angle caltrop shell, and that in ethanol extract was higher than that in water extract; the ethanol
extract from two angle caltrop shell was stronger to inhibit the A549 than water extract was (P<
0.01), while the water extract from corners caltrop shell was stronger to inhibit the A549 than two
angle caltrop was (P<0.01). Conclusion All the extracts fromcaltrop are very effective to inhibit
lung cancer cell line A549, the effect of ethanol extract is superior to the water extract and the
effect of those from Trapa natans L. Var. incisa Makino shell also higher than that from Trapa bicornis Var. bispinosa shell. |
| Key words: two angle shel corners caltrop shell A549 cells Folin assay tetramethy-
lammonium fluoride tetrazolium method
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