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柯超, 单生涛, 谭艳, 曹洋, 谢峥嵘, 周卓, 袁柳媚, 潘江, 章薇.电针心经/心包经穴对AD大鼠海马组织谷氨酸转运体的影响[J].湖南中医药大学学报英文版,2025,45(8):1469-1475.[Click to copy
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| 电针心经/心包经穴对AD大鼠海马组织谷氨酸转运体的影响 |
| 柯超,单生涛,谭艳,曹洋,谢峥嵘,周卓,袁柳媚,潘江,章薇 |
| (湖南中医药大学第一附属医院, 湖南 长沙 410007) |
| 摘要: |
| 目的 观察电针心经/心包经穴对阿尔茨海默病（AD）大鼠行为、海马区突触超微结构及老年斑沉积、海马组织谷氨酸转运体的影响。方法 将40只雄性SD大鼠随机分为假手术组10只、造模组30只，以双侧大脑海马CA1区内注射β淀粉样蛋白1-42(Aβ1-42)构建AD模型，术后假手术组死亡1只、造模组死亡3只，再将造模成功的27只大鼠随机分为模型组、心经穴组、心包经穴组，每组9只。心经组、心包经组进行相应的电针干预，假手术组、模型组仅捆绑处理不进行电针干预。采用Morris水迷宫实验观察大鼠学习记忆能力的变化，采用透射电镜观察大鼠海马区突触超微结构的变化，采用刚果红染色观察海马区Aβ斑块状的沉积，采用Western blot检测大鼠海马组织囊泡型谷氨酸转运蛋白2（VGLUT2）和兴奋性氨基酸转运蛋白2（EAAT2）的表达水平。结果 与假手术组相比，模型组大鼠穿越平台次数和目标象限停留时间均减少（P<0.01），突触超微结构受损，Aβ斑块状沉积程度加重，VGLUT2、EAAT2蛋白表达水平均下降（P<0.01）；与模型组相比，心经穴组、心包经穴组穿越平台次数和目标象限停留时间均增加（P<0.05,P<0.01），突触超微结构改善，Aβ斑块状沉积程度逐渐减轻，VGLUT2、EAAT2蛋白表达水平均上升（P<0.05,P<0.01）；心经穴组各项指标与心包经穴组相比，差异均无统计学意义（P>0.05）。结论 电针心经/心包经可改善AD大鼠空间学习记忆能力，减轻老年斑沉积及突触损伤，其作用机制可能与调控谷氨酸转运体有关。 |
| 关键词: 阿尔茨海默病 电针 心经穴/心包经穴 谷氨酸转运体 突触超微结构 囊泡型谷氨酸转运蛋白2 兴奋性氨基酸转运蛋白2 |
| DOI：10.3969/j.issn.1674-070X.2025.08.010 |
| Received:March 13, 2025 |
| 基金项目:国家自然科学基金项目(81973956);国家自然科学基金青年项目(82405580);湖南省自然科学基金项目(2025JJ60638);长沙市自然科学基金项目(kq2403097) |
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| Effects of electroacupuncture at heart meridian/pericardium meridian acupoints on glutamate transporters in hippocampal tissue of AD rats |
| KE Chao, SHAN Shengtao, TAN Yan, CAO Yang, XIE Zhengrong, ZHOU Zhuo, YUAN Liumei, PAN Jiang, ZHANG Wei |
| (The First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China) |
| Abstract: |
| Objective To investigate the effects of electroacupuncture（EA） at heart meridian/pericardium meridian acupoints on behavior, hippocampal synaptic ultrastructure, senile plaque deposition, and glutamate transporters in the hippocampal region of rats with Alzheimer’s disease（AD）. Methods Forty male SD rats were randomly divided into sham-operated group（n=10） and model group（n=30）. AD models were established by bilateral hippocampal CA1 region injection of β-amyloid protein 1-42(Aβ1-42). Postoperatively,one rat died in the sham-operated group and three in the model group. The remaining 27 successfully modeled rats were then randomized into model group, heart meridian acupoint group, and pericardium meridian acupoint group, with nine rats in each group. EA was applied to the heart meridian and pericardium meridian groups, while the sham-operated and model groups only underwent restraint without EA. Morris water maze test was used to observe the changes in learning and memory abilities of rats.Transmission electron microscopy was employed to observe the changes of synaptic ultrastructure in hippocampal region of rats.Congo red staining was used to examine Aβ plaque deposition in hippocampal region of rats. Western blot was performed to examine the expression levels of vesicular glutamate transporter 2（VGLUT2） and excitatory amino acid transporter 2（EAAT2） in hippocampal tissue of rats. Results Compared with the sham-operated group, the model group exhibited reduced number of platform crossings and shorter duration in target quadrant（P＜0.01）, impaired synaptic ultrastructure, aggravated Aβ plaque deposition, and reduced protein expression levels of VGLUT2 and EAAT2（P＜0.01）. Compared with the model group, both EA groups showed increased number of platform crossings and prolonged duration in target quadrant（P＜0.05, P＜0.01）, improved synaptic ultrastructure,reduced Aβ plaque deposition, and elevated protein expression levels of VGLUT2 and EAAT2（P＜0.05, P＜0.01）. No statistically significant differences were observed in any indicators between the heart meridian and pericardium meridian acupoint groups（P＞0.05）. Conclusion EA at heart/pericardium meridian acupoints improves spatial learning and memory in AD rats, reduces senile plaque deposition and synaptic damage, with mechanisms potentially linked to glutamate transporter regulation. |
| Key words: Alzheimer’s disease electroacupuncture heart meridian/pericardium meridian glutamate transporter synaptic ultrastructure vesicular glutamate transporter 2 excitatory amino acid transporter 2 |
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