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何伟, 官菊梅, 唐旭.火针通过TRPV1/PKC-δ/p-Akt信号通路调节带状疱疹后神经痛大鼠痛觉敏化的机制研究[J].湖南中医药大学学报英文版,2025,45(7):1271-1279.[Click to copy
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| 火针通过TRPV1/PKC-δ/p-Akt信号通路调节带状疱疹后神经痛大鼠痛觉敏化的机制研究 |
| 何伟,官菊梅,唐旭 |
| (四川中医药高等专科学校, 四川 绵阳 621000) |
| 摘要: |
| 目的 基于瞬时受体电位香草酸受体1（TRPV1）/蛋白激酶C-δ（PKC-δ）/磷酸化Akt蛋白（p-Akt）信号通路探讨火针疗法对带状疱疹后神经痛（PHN）大鼠的潜在作用机制。方法 采用香草素受体激动剂（RTX）腹腔注射法构建PHN大鼠模型。将成功建模的24只大鼠随机分为3组：模型组（RTX）、阳性药物组（RTX+加巴喷丁）、火针组（RTX+火针疗法），每组8只。再随机选取8只健康大鼠为对照组。采用机械缩足反射阈值（PWT）和热缩足反射潜伏期（TWL）评估火针疗法对PHA大鼠的痛敏程度；采用HE染色评估大鼠脊髓背角组织病理变化；采用TUNEL染色检测脊髓背角GABA能神经元细胞凋亡情况；采用ELISA检测大鼠脊髓背角组织中肿瘤坏死因子-α（TNF-α）和白细胞介素-1β（IL-1β）的含量；采用Western blot检测各组大鼠脊髓背角组织中关键蛋白TRPV1、PKC-δ、p-Akt表达。结果 与对照组相比，模型组PWT值降低（P<0.05），TWL值升高（P<0.05），GABA细胞凋亡数量增多（P＜0.05），TNF-α、IL-1β含量升高（P<0.05），TRPV1、PKC-δ、p-Akt表达升高（P<0.05）；与模型组相比，阳性药物组和火针组PWT值升高（P<0.05），TWL值降低（P<0.05），GABA细胞凋亡数量减少（P＜0.05），TNF-α、IL-1β含量降低（P<0.05），TRPV1、PKC-δ、p-Akt表达降低（P<0.05）；阳性药物组与火针组相比，PWT值、TWL值、GABA细胞凋亡数量以及TNF-α、IL-1β、TRPV1、PKC-δ、p-Akt水平差异均无统计学意义（P＞0.05）。结论 火针疗法通过抑制TRPV1/PKC-δ/p-Akt信号通路，减轻PHN大鼠炎症反应，进而缓解PHN痛觉敏化。 |
| 关键词: 带状疱疹后神经痛 香草素受体激动剂 火针 TRPV1/PKC-δ/p-Akt信号通路 肿瘤坏死因子-α 白细胞介素-1β 痛觉敏化 |
| DOI：10.3969/j.issn.1674-070X.2025.07.010 |
| Received:December 11, 2024 |
| 基金项目:四川省医学（青年创新）科研课题（Q20217）。 |
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| Mechanism of fire needling on regulating pain sensitization in rats with postherpetic neuralgia via TRPV1/PKC-δ/p-Akt signaling pathway |
| HE Wei, GUAN Jumei, TANG Xu |
| (Sichuan College of Traditional Chinese Medicine, Mianyang, Sichuan 621000, China) |
| Abstract: |
| Objective To explore the potential mechanism of action of fire needling therapy on rats with postherpetic neuralgia (PHN) based on the transient receptor potential vanilloid 1 (TRPV1)/protein kinase C-δ (PKC-δ)/phosphorylated Akt protein (p-Akt) signaling pathway. Methods A PHN rat model was established by intraperitoneal injection of vanilloid receptor agonist (resiniferatoxin, RTX). Twenty-four successfully modeled rats were randomly divided into the model group (RTX), positive drug group (RTX+gabapentin), and fire needling group (RTX+fire needle therapy), with eight rats in each group. Another eight healthy rats were randomly selected as the control group. Mechanical paw withdrawal threshold (PWT) and paw thermal withdrawal latency (TWL) were employed to evaluate the degree of hyperalgesia in PHN rats treated with fire needling therapy. HE staining was used to assess histopathological changes in the spinal dorsal horn of rats. TUNEL staining was used to examine apoptosis of GABAergic neurons in the spinal dorsal horn of rats. ELISA was used to measure the content of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in the spinal dorsal horn tissue of rats. Western blot was used to measure the expressions of key proteins TRPV1, PKC-δ, and p-Akt in the spinal dorsal horn tissue of rats in each group. Results Compared with the control group, the model group exhibited lower PWT values (P<0.05), higher TWL values (P<0.05), increased quantity of apoptotic GABA cells (P<0.05), elevated levels of TNF-α and IL-1β (P<0.05), and increased expressions of TRPV1, PKC-δ, and p-Akt (P<0.05). Compared with the model group, both the positive drug group and the fire needling group showed increased PWT values (P<0.05), decreased TWL values (P<0.05), decreased quantity of apoptotic GABA cells (P<0.05), decreased levels of TNF-α and IL-1β (P<0.05), and decreased expressions of TRPV1, PKC-δ, and p-Akt (P<0.05). There were no statistically significant differences in PWT values, TWL values, quantity of apoptotic GABA cells, and levels of TNF-α, IL-1β, TRPV1, PKC-δ, and p-Akt between the positive drug group and the fire needling group (P>0.05). Conclusion Fire needling therapy alleviates the inflammatory response of PHN rats by inhibiting the TRPV1/PKC-δ/p-Akt signaling pathway, thereby relieving pain sensitization in PHN. |
| Key words: postherpetic neuralgia vanilloid receptor agonist fire needling TRPV1/PKC-δ/p-Akt signaling pathway tumor necrosis factor-α interleukin-1β pain sensitization |
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