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徐小珊, 田岳凤, 熊罗节, 翟春涛, 李玮.经方隔药饼灸对免疫抑制与慢性疲劳综合征模型大鼠免疫器官及免疫因子的作用研究[J].湖南中医药大学学报英文版,2025,45(10):1884-1892.[Click to copy
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| 经方隔药饼灸对免疫抑制与慢性疲劳综合征模型大鼠免疫器官及免疫因子的作用研究 |
| 徐小珊,田岳凤,熊罗节,翟春涛,李玮 |
| (湖南中医药大学针灸推拿与康复学院, 湖南 长沙 410208;山西中医药大学第二临床学院, 山西 晋中 030619) |
| 摘要: |
| 目的 观察经方隔药饼灸干预对免疫抑制与慢性疲劳综合征模型大鼠免疫器官与免疫因子的影响。方法 选取80只SD大鼠，针对免疫低下的两种疾病模型（免疫抑制、慢性疲劳综合征）分别进行独立造模，再进行随机分组，每种疾病模型均分为空白组、模型组、隔药饼灸组、假药饼灸组，每组10只。空白组与模型组不进行治疗干预；免疫抑制模型中隔药饼灸组与假药饼灸组选择神阙、关元、中脘、足三里（双）进行六味地黄汤组方隔药饼灸治疗；慢性疲劳综合征模型中隔药饼灸组与假药饼灸组选择神阙、关元、足三里（双）、期门（双）进行逍遥散组方隔药饼灸治疗。均干预10 d。旷场实验观察各组大鼠行为学变化；ELISA检测血清中吞噬细胞膜糖蛋白（CD44）、L-选择素（CD62L）、干扰素-γ（IFN-γ）、肿瘤坏死因子-α（TNF-α）、白细胞介素（IL）-10及IL-2含量；HE染色观察脾脏、胸腺组织形态学变化。结果 与空白组比较，模型组大鼠的运动时间、运动距离以及中央格停留时间显著减少（P＜0.01）；血清中CD44、CD62L、IFN-γ、TNF-α含量显著升高，IL-2、IL-10含量显著下降（P＜0.01）；脾脏、胸腺组织结构受损；脾脏指数显著升高，胸腺指数显著降低（P＜0.01）。与模型组比较，隔药饼灸组与假药饼灸组大鼠的运动时间以及中央格停留时间增加（P＜0.05，P＜0.01）；血清中CD62L、IFN-γ、TNF-α含量下降，IL-10含量升高（P＜0.05，P＜0.01）；脾脏、胸腺组织损伤减轻；脾脏指数降低，胸腺指数升高（P＜0.05，P＜0.01）。与隔药饼灸组比较，假药饼灸组大鼠的运动时间、运动距离以及中央格停留时间减少（P＜0.05，P＜0.01）；血清中CD44、TNF-α含量升高，IL-10含量下降（P＜0.05）；脾脏、胸腺组织病损恢复较差；脾脏指数升高（P＜0.05），胸腺指数差异无统计学意义（P＞0.05）。结论 隔药饼灸可显著改善免疫低下（免疫抑制与慢性疲劳综合征）模型大鼠的免疫功能，其效果优于假药饼灸，可能与不同经方药饼的药物作用相关。 |
| 关键词: 慢性疲劳综合征 免疫抑制 六味地黄汤 逍遥散 隔药饼灸 假药饼灸 |
| DOI：10.3969/j.issn.1674-070X.2025.10.011 |
| Received:April 13, 2025 |
| 基金项目:国家自然科学基金项目（82174504，81674062）。 |
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| Effects of classic formula-based moxibustion on herbal cake on immune organs and immune factors in rat models of immunosuppression and chronic fatigue syndrome |
| XU Xiaoshan, TIAN Yuefeng, XIONG Luojie, ZHAI Chuntao, LI Wei |
| (School of Acupuncture-moxibustion, Tuina and Rehabilitation, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The Second Clinical College, Shanxi University of Chinese Medicine, Jinzhong, Shanxi 030619, China) |
| Abstract: |
| Objective To observe the effects of classic formula-based moxibustion on herbal cake on immune organs and immune factors in rat models of immunosuppression and chronic fatigue syndrome. Methods Eighty SD rats were selected. Independent modeling was performed for two immunocompromised disease models (i.e., immunosuppression and chronic fatigue syndrome), followed by random grouping. Each disease model was divided into blank group, model group, moxibustion on herbal cake group and sham moxibustion on herbal cake group, with ten rats in each group. The blank and model groups received no therapeutic intervention. For the immunosuppression model, both the moxibustion on herbal cake group and the sham moxibustion on herbal cake group were treated with Liuwei Dihuang Decoction-based and excipient-based moxibustion on herbal cake, respectively, at the acupoints of Shenque (CV8), Guanyuan (CV4), Zhongwan (CV12), and Zusanli (ST36, bilateral). For the chronic fatigue syndrome model, the moxibustion on herbal cake group and the sham moxibustion on herbal cake group were treated with Xiaoyao Powder-based and excipient-based moxibustion on herbal cake, respectively, at the acupoints of Shenque (CV8), Guanyuan (CV4), Zusanli (ST36, bilateral), and Qimen (LR14, bilateral). All interventions lasted for ten days. The behavioral changes of rats in each group were observed using the open field test. The serum levels of cluster of differentiation 44 (CD44), cluster of differentiation 62 L-selectin (CD62L), interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), interleukin (IL)-10, and IL-2 were measured by ELISA. The morphological changes in the spleen and thymus tissue were observed by HE staining. Results Compared with the blank group, the model group showed significant decreases in movement time, movement distance, and central grid residence time (P＜0.01). The serum levels of CD44, CD62L, IFN-γ, and TNF-α significantly increased, while those of IL-2 and IL-10 significantly decreased (P＜0.01). The histological structure of the spleen and thymus was damaged. The spleen index significantly increased, and the thymus index significantly decreased (P＜0.01). Compared with the model group, the moxibustion on herbal cake group and the sham moxibustion on herbal cake group showed increased movement time, and central grid residence time (P＜0.05, P＜0.01). The serum levels of CD62L, IFN-γ, and TNF-α decreased, while that of IL-10 increased (P＜0.05, P＜0.01). The damage of histological structure of the spleen and thymus was alleviated. The spleen index decreased, and the thymus index increased (P＜0.05, P＜0.01). Compared with the moxibustion on herbal cake group, the sham moxibustion on herbal cake group showed decreased movement time, movement distance, and central grid residence time (P＜0.05, P＜0.01). The serum levels of CD44 and TNF-α were higher, and IL-10 level was lower (P＜0.05). Pathological recovery in the spleen and thymus was poorer. The spleen index was higher (P＜0.05), while no statistically significant difference was found in the thymus index (P>0.05). Conclusion Classic formula-based moxibustion on herbal cake can significantly improve immune function in rat models of immunocompromise (immunosuppression and chronic fatigue syndrome). Its efficacy is superior to that of sham moxibustion on herbal cake, which may be related to the pharmacological actions of different classical formula-based herbal cakes. |
| Key words: chronic fatigue syndrome immunosuppression Liuwei Dihuang Decoction Xiaoyao Powder moxibustion on herbal cake sham moxibustion on herbal cake |
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