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肖孟霞, 刘超, 田安哲, 郭城, 刘红华, 陈昕羽, 易洪芬, 葛君芸, 刘迈兰.针刺对动脉粥样硬化兔脂质代谢及肠道菌群的影响[J].湖南中医药大学学报英文版,2025,45(11):2116-2124.[Click to copy
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| 针刺对动脉粥样硬化兔脂质代谢及肠道菌群的影响 |
| 肖孟霞,刘超,田安哲,郭城,刘红华,陈昕羽,易洪芬,葛君芸,刘迈兰 |
| (湖南中医药大学针灸推拿与康复学院, 湖南 长沙 410208;湖南中医药大学第二附属医院, 湖南 长沙 410005;湖南中医药大学第一附属医院, 湖南 长沙 410007;湖南中医药大学护理学院, 湖南 长沙 410208) |
| 摘要: |
| 目的 探讨针刺对高脂饮食诱导的动脉粥样硬化（AS）兔脂质代谢和肠道菌群的影响及其作用机制。方法 24只新西兰兔随机分为空白组、模型组与针刺组，每组8只。空白组正常饲料喂养；模型组高脂饲料喂养；针刺组高脂饲料喂养并针刺干预，每天干预1次，连续12周。全自动生化仪检测血清总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-C）以及高密度脂蛋白胆固醇（HDL-C）含量；HE染色和油红O染色观察兔主动脉病理变化及斑块面积；16S rDNA测序分析肠道菌群结构及功能。结果 与空白组比较，模型组TC、TG、LDL-C含量均升高（P<0.01）,HDL-C含量降低（P<0.01）；胸主动脉斑块面积比升高（P<0.05）。与模型组比较，针刺组TC、TG、LDL-C含量均下降（P<0.01）,HDL-C含量升高（P<0.05）；胸主动脉斑块面积比降低（P<0.05）。16S rDNA测序结果表明，针刺可调节AS兔的肠道菌群结构，并影响菌群α和β多样性。与空白组比较，模型组厚壁菌门（Firmicutes）、NK4A214＿group菌属相对丰度升高（P<0.05）；拟杆菌门（Bacteroidota）、蓝藻菌门（Cyanobacteria）、穆里杆菌属（Muribaculacea）、拟杆菌属（Bacteroides）相对丰度降低（P<0.05）。与模型组比较，针刺组拟杆菌门、蓝藻菌门、穆里杆菌属、拟杆菌属相对丰度升高（P<0.05）;NK4A214_group菌属相对丰度下降（P<0.05）。KEGG功能预测显示，与空白组比较，模型组其他次级代谢产物的生物合成（Biosynthesis of other secondary metabolites）和糖链生物合成与代谢（Glycan biosynthesis and metabolism）相对丰度均降低（P<0.05）。与模型组比较，针刺组糖链生物合成与代谢相对丰度升高（P<0.05）。结论 针刺可能通过调节肠道菌群多样性和结构，改善AS兔脂质代谢及主动脉损伤；同时通过影响糖链生物合成与代谢功能，促进内皮功能恢复，从而发挥抗AS作用。 |
| 关键词: 动脉粥样硬化 针刺 16S rDNA测序 肠道菌群 脂质代谢 |
| DOI：10.3969/j.issn.1674-070X.2025.11.012 |
| Received:July 03, 2025 |
| 基金项目:国家自然科学基金项目(82074559); 湖南省重点研发计划项目(2024JK2130); 湖南省青年科技创新人才项目(2022RC1222); 长沙市杰出创新青年培养计划项目(kq1905036); 湖南省教育厅重点研发项目(24A0282)。 |
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| Influence of acupuncture on lipid metabolism and gut microbiota in rabbits with atherosclerosis |
| XIAO Mengxia, LIU Chao, TIAN Anzhe, GUO Cheng, LIU Honghua, CHEN Xinyu, YI Hongfen, GE Junyun, LIU Mailan |
| (School of Acupuncture-moxibustion, Tuina and Rehabilitation, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The Second Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410005, China;The First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;School of Nursing, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To explore the effects of acupuncture on lipid metabolism and gut microbiota in rabbits with atherosclerosis（AS） induced by a high-fat diet and its mechanism of action. Methods Twenty-four New Zealand rabbits were randomly divided into blank group, model group, and acupuncture group, with 8 rabbits in each group. The blank group was fed with a normal diet, the model group was fed with a high-fat diet, and the acupuncture group was fed with high-fat diet combined with daily acupuncture intervention, once daily for 12 weeks. Serum levels of total cholesterol（TC）, triglycerides（TG）, low-density lipoprotein cholesterol（LDL-C）, and high-density lipoprotein cholesterol（HDL-C） were measured using a fully automated biochemistry analyzer. Pathological changes in the rabbit aortas and plaque areas were observed through hematoxylin-eosin（HE） staining and oil red O staining. The structure and function of the gut microbiota were analyzed by 16S rDNA sequencing. Results Compared with the blank group, the levels of TC, TG, and LDL-C increased（P＜0.01）, the HDL-C levels decreased（P＜0.01）, and the ratio of thoracic aortic plaque area increased（P＜0.05） in the model group. Compared with the model group, the levels of TC, TG, and LDL-C decreased（P＜0.01）, the HDL-C levels increased（P＜0.05）, and the ratio of thoracic aortic plaque area decreased（P＜0.05） in the acupuncture group. The results of 16S rDNA sequencing indicated that acupuncture could modulate the gut microbiota structure and influence both α and β diversity in AS rabbits. Compared with the blank group, the model group demonstrated increased relative abundances of Firmicutes and the NK4A214＿group genus（P ＜0.05）, while showing decreased relative abundances of Bacteroidota, Cyanobacteria, Muribaculacea, and Bacteroides（P＜0.05）. In comparison with the model group, the acupuncture group exhibited increased relative abundances of Bacteroidota, Cyanobacteria, Muribaculacea, and Bacteroides（P ＜0.05）, along with a decreased relative abundance of the NK4A214＿group genus（P＜0.05）. KEGG functional predictions revealed that, compared with the blank group, the model group had reduced relative abundances of Biosynthesis of other secondary metabolites and Glycan biosynthesis and metabolism（P ＜0.05）. Conversely, compared with the model group, the acupuncture group showed an increased relative abundance of Glycan biosynthesis and metabolism（P＜0.05）. Conclusion Acupuncture may improve lipid metabolism and alleviate aortic injury in AS rabbits by modulating the diversity and structure of the gut microbiota. Meanwhile, it may promote endothelial function recovery by influencing carbohydrate chain biosynthesis and metabolic functions, thereby exerting antiatherosclerotic effects. |
| Key words: atherosclerosis acupuncture 16S rDNA sequencing gut microbiota lipid metabolism |
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