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付港, 肖时雨, 姚清颖, 张在其, 江星明, 李斌.三叶委陵菜化学成分及其抗氧化活性研究[J].湖南中医药大学学报英文版,2025,45(3):460-466.[Click to copy
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| 三叶委陵菜化学成分及其抗氧化活性研究 |
| 付港,肖时雨,姚清颖,张在其,江星明,李斌 |
| (湖南中医药大学药学院, 湖南省中医药民族医药国际联合实验室, 湖南 长沙 410208;湖南中医药大学药学院, 湖南省天然药物资源与功能实验室, 湖南 长沙 410208;湖南医药学院侗医药研究湖南省重点实验室, 湖南 怀化 418000) |
| 摘要: |
| 目的 研究三叶委陵菜（Potentilla freyniana Bornm.）根二氯甲烷部位和乙酸乙酯部位的化学成分，并筛选其抗氧化活性成分。方法 采用硅胶柱色谱、凝胶柱色谱、半制备高效液相等色谱技术分离纯化其醇提物的二氯甲烷和乙酸乙酯萃取部位，利用核磁共振波谱、质谱等技术对所得化合物的结构进行鉴定。通过羟自由基清除实验、DPPH自由基清除实验以及ABTS自由基清除实验，对黄酮类化合物7和9的抗氧化活性展开测定。结果 从三叶委陵菜中分离得到17个化合物，鉴定为胡萝卜苷（1）、β-谷甾醇（2）、7-酮-β-谷甾醇（3）、柚皮素（4）、红花素（5）、香橙素（6）、圣草酚（7）、槲皮素（8）、儿茶素（9）、thunberginol C（10）、杜鹃醇（11）、4-(4-carboxy-2-methoxyphenoxy)-3,5-dimethoxybenzoic acid（12）、邻苯二甲酸丁二酯（13）、香草酸（14）、原儿茶酸（15）、对羟基苯甲酸（16）、莲花掌苷（17）。其中，化合物4为首次从该植物中分离得到，化合物3、10、12、13、14为首次从该属植物中分离得到。化合物7（浓度大于5 mmol/L）和化合物9（浓度大于2 mmol/L）两个化合物对羟自由基的清除率均超过90%、对DPPH自由基和ABTS自由基的清除率均超过95%，抗氧化水平与维生素C相近。结论 化合物7和9在抗氧化和抗炎潜力方面表现了一定活性潜力，为三叶委陵菜的化学成分和生物活性提供了一些实验依据和科学参考，推动了其活性成分的进一步开发与利用。 |
| 关键词: 委陵菜属 三叶委陵菜 化学成分 甾体 黄酮 抗氧化 |
| DOI：10.3969/j.issn.1674-070X.2025.03.010 |
| Received:December 27, 2024 |
| 基金项目:湖南医药学院侗医药研究湖南省重点实验室（2017CT5025）；湖南中医药大学研究生创新项目（2022CX71）。 |
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| Chemical constituents and antioxidant activity of Potentilla freyniana Bornm |
| FU Gang, XIAO Shiyu, YAO Qingying, ZHANG Zaiqi, JIANG Xingming, LI Bin |
| (TCM and Ethnomedicine Innovation & Development International Laboratory, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hunan Province Laboratory of Natural Medicinal Resources and Functions, School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hunan Provincial Key Laboratory of Dong Medicine, Hunan University of Medicine, Huaihua, Hunan 418000, China) |
| Abstract: |
| Objective To study the chemical constituents of the dichloromethane and ethyl acetate fractions of the roots of Potentilla freyniana Bornm. and to screen for their antioxidant active components. Methods The dichloromethane and ethyl acetate fractions of the ethanol extract were separated and purified using silica gel chromatography, gel chromatography, and semi-preparative HPLC. The structures of the isolated compounds were identified by NMR and MS. The antioxidant activities of the flavonoids 7 and 9 were evaluated through hydroxyl radical scavenging assay, DPPH radical scavenging assay, and ABTS radical scavenging assay. Results Seventeen compounds were isolated from P. freyniana and identified as daucosterol (1), β-sitosterol (2), 7-keto-β-sitosterol (3), naringenin (4), carthamidin (5), dihydrokaempferol (6), eriodictyol (7), quercetin (8), catechin (9), thunberginol C (10), rhododenol (11), 4-(4-carboxy-2-methoxyphenoxy)-3,5-dimethoxybenzoic acid (12), butylene phthalate (13), vanillic acid (14), protocatechuic acid (15), phydroxybenzoic acid (16), and lindleyin (17). Among them, compound 4 was identified from this plant for the first time, and compounds 3, 10, 12, 13, and 14 were separated from this genus for the first time. When the concentration of compound 7 exceeds 5 mmol/L and the concentration of compound 9 exceeds 2 mmol/L, the scavenging rate of hydroxyl radicals exceeds 90%, and the scavenging rates of DPPH radicals and ABTS radicals exceed 95%. The antioxidant level is similar to that of VC, indicating significant antioxidant performance. Conclusion Compounds 7 and 9 exhibited certain potential in antioxidant and anti-inflammatory activities. This study provides some experimental basis and scientific references for the chemical composition and biological activity of Potentilla freyniana Bornm., promoting the further development and utilization of its active components. |
| Key words: Potentilla L Potentilla freyniana Bornm. chemical constituents steroid flavone antioxidantion |
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