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龙伽雯, 侯凤飞, 曾贵荣, 戴鑫汶, 周融融, 徐琳本, 李跃辉.不同工艺的银苓通脉健脑颗粒对大鼠血管性痴呆模型定位航行的影响[J].湖南中医药大学学报英文版,2025,45(2):220-227.[Click to copy
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| 不同工艺的银苓通脉健脑颗粒对大鼠血管性痴呆模型定位航行的影响 |
| 龙伽雯,侯凤飞,曾贵荣,戴鑫汶,周融融,徐琳本,李跃辉 |
| (湖南中医药大学, 湖南 长沙 410208;湖南省中医药研究院, 湖南 长沙 410013;湖南补天药业股份有限公司, 湖南 怀化 418400;湖南普瑞玛药物研究中心有限公司, 湖南 长沙 410003) |
| 摘要: |
| 目的 本实验采用改良双血管阻断法建立大鼠血管性痴呆（VD）模型，比较不同提取工艺银苓通脉健脑颗粒对VD模型大鼠的治疗作用，筛选出更佳药效作用的提取工艺路线，为新药研发提供依据。方法 采用HPLC建立莫诺苷、马钱苷含量测定方法，检测样品中莫诺苷、马钱苷含量。采用大鼠双侧颈部血管结扎法（2-VO）建立VD模型，分别设置正常对照组、假手术组、模型对照组、多奈哌齐组以及3个不同工艺样品组。采用Morris水迷宫实验观察各组定位航行潜伏期、穿越平台次数；采用HE染色与尼氏染色观察大鼠海马组织病理形态学变化。结果 莫诺苷、马钱苷的回归方程分别为Y=17.431X+5.369 4，r=1。Y=16.035X+9.730 4，r=0.999 9。分别在18.55～185.47 μg/mL、19.38～193.84 μg/mL范围内具有良好的线性关系。精密度、重复性、加样回收率均较好（RSD均小于3%），阴性样品无干扰，3个样品莫诺苷、马钱苷含量之和分别为：6.93、6.87、5.96 mg/g。Morris水迷宫实验结果显示，与模型对照组比较，工艺②、③号样组大鼠定位航行第3、第4天潜伏期增加（P≤0.05），工艺②号样能显著增加大鼠穿越平台次数；HE染色结果显示工艺②号样组大鼠海马组织部分海马锥体细胞排列紧密；少量细胞间质水肿；尼氏染色结果显示，与模型对照组比较，工艺②号样组大鼠海马组织CA1区尼氏小体数量明显增加。结论 工艺②号样的药效最佳，为银苓通脉健脑颗粒新药研发的制备工艺提供了实验数据。 |
| 关键词: 银苓通脉健脑颗粒 提取工艺 血管性痴呆 莫诺苷 马钱苷 |
| DOI：10.3969/j.issn.1674-070X.2025.02.005 |
| Received:July 08, 2024 |
| 基金项目:湖南省自然科学基金项目（2024JJ7292）；湖南省科技厅科技创新引领计划（2020SK2051）；湘卫函〔2024〕43号（青年骨干人才）；湘中医药〔2024〕3号（骨干人才）。 |
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| Effects of Yinling Tongmai Jiannao Granule prepared by different processes on spatial navigation ability in rat models of vascular dementia |
| LONG Jiawen, HOU Fengfei, ZENG Guirong, DAI Xinwen, ZHOU Rongrong, XU Linben, LI Yuehui |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hunan Academy of Chinese Medicine, Changsha, Hunan 410013, China;Hunan Butian Pharmaceutical Co., LTD., Huaihua, Hunan 418400, China;Hunan Prima Pharmaceutical Research Center Co., LTD., Changsha, Hunan 410003, China) |
| Abstract: |
| Objective To compare the therapeutic effects of Yinling Tongmai Jiannao Granule prepared by different extraction processes on rat models of vascular dementia (VD) established by modified two-vessel occlusion method, and identify the optimal extraction process route with better pharmacological efficacy, so as to provide a basis for new drug development. Methods An HPLC method was established for the determination of morroniside and loganin content in the samples. The VD model was established using the two-vessel occlusion (2-VO) method in rats. Groups were set up including a normal control group, a sham-operated group, a model control group, a donepezil group, and three groups receiving different processing samples (sample 1 group, sample 2 group, and sample 3 group). The Morris water maze test was employed to observe the latency to find the platform and the number of platform crossings in each group. Additionally, HE staining and Nissl staining were performed to observe the histopathological changes in hippocampal tissue of rats. Results The regression equations for morroniside and loganin were Y=17.431X+5.3694, r=1, and Y=16.035X+9.730 4, r=0.999 9, respectively, demonstrating good linearity within the ranges of 18.55-185.47 μg/ml and 19.38-193.84 μg/mL. The precision, repeatability, and recovery rates were good (RSD<3% for all), with no interference from negative samples. The total content of morroniside and loganin in the three samples was 6.93 mg/g, 6.87 mg/g, and 5.96 mg/g, respectively. The results of Morris water maze test showed that compared with the model control group, the latency to locate the platform on the third and fourth days increased in rats of sample 2 and sample 3 groups (P≤0.05), with sample 2 group showing a notable increase in the number of platform crossings. HE staining showed that some hippocampal pyramidal cells in the hippocampus of rats of sample 2 group were tightly arranged with slight interstitial edema. Nissl staining revealed a significant increase in the number of Nissl bodies in the CA1 region of the hippocampus in rats of sample 2 group compared with the model control group. Conclusion The processed sample 2 exhibited the best therapeutic effects, providing experimental data for the preparation process of Yinling Tongmai Jiannao Granule in new drug research and development. |
| Key words: Yinling Tongmai Jiannao Granule extraction process vascular dementia morroniside loganin |
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