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唐玉莹, 段雨倩, 王适, 何若斓, 谭诗淼, 邹文娟, 向丽萍.清脂固发酊对雄激素性脱发小鼠模型毛发生长的影响[J].湖南中医药大学学报英文版,2025,45(2):196-203.[Click to copy
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| 清脂固发酊对雄激素性脱发小鼠模型毛发生长的影响 |
| 唐玉莹,段雨倩,王适,何若斓,谭诗淼,邹文娟,向丽萍 |
| (湖南中医药大学, 湖南 长沙 410208;湖南中医药大学第二附属医院, 湖南 长沙 410000) |
| 摘要: |
| 目的 探讨清脂固发酊对雄激素性脱发（AGA）小鼠模型毛发生长的影响及作用机制。方法 将70只SPF级C57BL/6小鼠随机分为空白组、模型组、乙醇组、米诺地尔酊组及低、中、高浓度清脂固发酊组。除空白组外，其余各组连续4周皮下注射丙酸睾酮注射液5 mg/（kg·d），建立AGA小鼠模型。造模成功后在脱毛区继续皮下注射等量丙酸睾酮注射液的同时涂抹相应药物，其中空白组及模型组涂抹生理盐水，米诺地尔酊组涂抹5%米诺地尔酊，乙醇组涂抹70%乙醇，低、中、高浓度清脂固发酊组分别涂抹0.1、0.2、0.4 g/mL清脂固发酊，各组均涂抹1 mL/次，每日1次，连续治疗6周。肉眼观察小鼠毛发生长情况；记录脱毛区皮色变黑时间；用药干预14、21、28、35、42 d随机拔取新生毛发5根，测量其长度；HE染色观察脱毛区毛囊形态，并计算毛囊数量及终毛/毳毛比值；ELISA检测血清中睾酮（T）、雌二醇（E2）水平，并计算两者比值。结果 与空白组比较，模型组毛发稀疏灰暗、毛囊密度稀疏，皮色变黑时间明显升高（P<0.01），各个时间点新生毛发长度明显缩短（P<0.01），毛囊数量和终毛/毳毛比值明显降低（P<0.01），血清中T水平及T/E2比值显著升高（P<0.01），E2水平显著降低（P<0.01）。与模型组比较，低、中、高浓度清脂固发酊组毛发旺盛有光泽、毛囊密度较大，高浓度清脂固发酊组脱毛区皮色变黑时间明显升高（P<0.01），低浓度清脂固发酊组在干预28、35、42 d时新生毛发长度增长（P<0.05，P<0.01），中、高浓度清脂固发酊组在各个时间点新生毛发长度明显增长（P<0.01）、毛囊数量和终毛/毳毛比值明显升高（P<0.01）、血清E2水平均显著升高（P<0.01），低、中、高浓度清脂固发酊组T水平及T/E2比值均降低（P<0.05，P<0.01）。与低浓度清脂固发酊组比较，中、高浓度清脂固发酊组毛发更浓密黑亮、毛囊密度大，中浓度清脂固发酊组在干预35、42 d新生毛发长度明显增长（P<0.01），高浓度清脂固发酊组在干预21、28、35、42 d新生毛发长度明显增长（P<0.01），中、高浓度清脂固发酊组毛囊数量和终毛/毳毛比值升高（P<0.05，P<0.01）、T水平及T/E2比值显著降低（P<0.01）、水平升高（P<0.05，P<0.01）。结论 清脂固发酊可以促使毛囊提前进入生长期，增加毛囊数量，改善毛囊微型化，加速毛发生长，其可能是通过调节激素水平以发挥作用。 |
| 关键词: 雄激素性脱发 脂溢性脱发 清脂固发酊 皮色变黑时间 新生毛发长度 毛囊数量 终毛/毳毛比值 激素水平 |
| DOI：10.3969/j.issn.1674-070X.2025.02.002 |
| Received:July 23, 2024 |
| 基金项目:湖南省科技厅项目基金（2021SK51305）。 |
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| Effects of Qingzhi Gufa Tincture on hair growth in a mouse model of androgenetic alopecia |
| TANG Yuying, DUAN Yuqian, WANG Shi, HE Ruolan, TAN Shimiao, ZOU Wenjuan, XIANG Liping |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The Second Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410000, China) |
| Abstract: |
| Objective To investigate the effects and mechanism of action of Qingzhi Gufa Tincture (QZGFT) on hair growth in androgenic alopecia (AGA) mouse model. Methods Seventy SPF C57BL/6 mice were randomly divided into blank group, mode group, ethanol group, minoxidil tincture group, and low-, medium-, and high-concentration QZGFT groups. Except for the blank group, the other groups were subcutaneously injected with testosterone propionate injection 5 mg/(kg·d) for four weeks to establish AGA mouse model. After successful modeling, while continuing to administer an equal amount of testosterone propionate subcutaneously in the depilation area, the corresponding medications were applied at the same time. The blank and model groups were treated with normal saline, the minoxidil tincture group with 5% minoxidil tincture, the ethanol group with 70% ethanol, and the low-, medium-, and high-concentration QZGFT groups with 0.1 g/mL, 0.2 g/mL, and 0.4 g/mL QZGFT, respectively. Each group received 1 mL per application, once daily for six consecutive weeks. The hair growth was observed macroscopically. The time for skin darkening in the depilation area was recorded. After 14, 21, 28, 35, and 42 days of drug intervention, five newly grown hairs were randomly plucked and their length was measured. HE staining was used to observe the morphology of hair follicles in the depilation area, and the number of hair follicles as well as the terminal to vellus hair ratio were calculated. ELISA was used to check the levels of testosterone (T) and estradiol (E2) in serum, and the ratio of the two was calculated. Results Compared with the blank group, the hair in the model group was sparse and gray, with small density of hair follicles. The time for skin darkening significantly increased (P<0.01), the length of newly grown hairs was significantly shortened at each time point (P<0.01), and the number of hair follicles and the terminal to vellus hair ratio were significantly lower (P<0.01). The serum T level and T/E2 ratio were significantly elevated (P<0.01), while the serum E2 level was significantly lower (P<0.01). Compared with the model group, the low-, medium-, and high-concentration QZGFT groups had bright and shiny hair, with greater follicle density; the time for skin darking in the depilation area of the high-concentration QZGFT group was significantly higher (P<0.01); the length of newly grown hairs in the low-concentration QZGFT group increased at 28, 35, and 42 days after intervention (P<0.05, P<0.01). At each time point, the length of newly grown hairs (P<0.01), the number of hair follicles and the terminal to vellus hair ratio were significantly higher (P<0.01), and the serum E2 level was significantly higher (P<0.01) in the medium- and high- concentration QZGFT groups. The T level and T/E2 ratio of low-, medium-, and high-concentration QZGFT groups were decreased (P<0.05, P<0.01). Compared with the low-concentration QZGFT group, the medium- and high-concentration QZGFT groups exhibited denser and shinier hair with greater follicle density..The length of newly grown hairs in the medium-concentration QZGFT group significantly increased on the 35 and 42 days of intervention (P<0.01). At 21, 28, 35, and 42 days after intervention, the length of newly grown hairs in the high-concentration QZGFT group significantly increased (P<0.01). The medium- and high-concentration QZGFT groups also had increased number of hair follicles and terminal to vellus hair ratio (P<0.05, P<0.01), significantly reduced T levels and T/E2 ratio (P<0.01), and elevated E2 level (P<0.05, P<0.01). Conclusion QZGFT can promote the early entry of hair follicles into the growth phase, increase the number of hair follicles, improve hair follicle miniaturization, and accelerate hair growth, which may exert its effects by regulating hormone levels. |
| Key words: androgenic alopecia seborrheic alopecia Qingzhi Gufa Tincture time for skin darkening length of newly grown hairs number of hair follicles terminal to vellus hair ratio hormone levels |
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