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廖卓, 包晴, 高元航, 李海英, 王璐, 唐林, 杨磊.整合指纹图谱与多成分含量测定的益肾固精暖脐贴质量评价研究[J].湖南中医药大学学报英文版,2025,45(1):59-64.[Click to copy
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| 整合指纹图谱与多成分含量测定的益肾固精暖脐贴质量评价研究 |
| 廖卓,包晴,高元航,李海英,王璐,唐林,杨磊 |
| (湖南中医药大学第一中医临床学院, 湖南 长沙 410007;湖南中医药大学第一附属医院, 湖南 长沙 410007) |
| 摘要: |
| 目的 建立益肾固精暖脐贴高效液相色谱法（HPLC）指纹图谱，并测定不同批次益肾固精暖脐贴中6种成分（桂皮醛、丁香酚、羟基-ɑ-山椒素、羟基-β-山椒素、母丁香酚、蛇床子素）的含量，为益肾固精暖脐贴质量控制提供参考。方法 采用高效液相色谱法建立15批益肾固精暖脐贴指纹图谱，进行相似度评价和正交偏最小二乘法-判别分析（OPLS-DA）筛选差异性成分，并对6种指标成分进行含量测定。结果 建立益肾固精暖脐贴HPLC指纹图谱及6种指标成分的含量测定方法，15个批次相似度范围为0.874～0.999，标定共有峰21个，指认出6个色谱峰，筛选出12个差异性成分。15批益肾固精暖脐贴中6种成分含量稳定。结论 建立的益肾固精暖脐贴指纹图谱及6种成分的含量测定方法准确、稳定、重复性好，可用于益肾固精暖脐贴的质量控制。 |
| 关键词: 益肾固精暖脐贴 指纹图谱 含量测定 质量评价 相似度评价 正交偏最小二乘法-判别分析 |
| DOI：10.3969/j.issn.1674-070X.2025.01.008 |
| Received:September 26, 2024 |
| 基金项目:湖南省自然科学基金项目(2022JJ80094,2024JJ6362);湖南省卫生健康高层次人才项目(湘卫函[2024]43号)。 |
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| Quality evaluation of Yishen Gujing Nuanqi Patch through integration of fingerprinting and multi-component content determination |
| LIAO Zhuo, BAO Qing, GAO Yuanhang, LI Haiying, WANG Lu, TANG Lin, YANG Lei |
| (The First Clinical School of Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;The First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China) |
| Abstract: |
| Objective To establish a high performance liquid chromatography（HPLC） fingerprint for Yishen Gujing Nuanqi Patch（YSGJNQP） and to determine the content of six components（cinnamic aldehyde, eugenol, hydroxy-ɑ-sanshool, hydroxy-β-sanshool, parent eugenol, and osthole） in different batches, so as to provide the reference for the quality control of YSGJNQP.Methods Fingerprints of 15 batches of YSGJNQP were established by HPLC. The similarity evaluation and orthogonal partial least square method-discriminant analysis（OPLS-DA） were used to screen out the differential components, and the content of six index components was determined. Results The HPLC fingerprint of YSGJNQP and the content determination method of the six index components were established. The similarity among 15 batches of samples ranged from 0.874 to 0.999, twenty-one common peaks and six chromatographic peaks were identified, and 12 differential components were screened out. The content of the six index components was found to be stable across the 15 batches of YSGJNQP. Conclusion The established HPLC fingerprint of YSGJNQP and the content determination method of its six components are accurate, stable, and reproducible. Consequently, it can be effectively used for the quality control of YSGJNQP. |
| Key words: Yishen Gujing Nuanqi Patch fingerprint content determination quality evaluation similarity evaluation orthogonal partial least square method-discriminant analysis |
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