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杨争, 胡玉蝶, 袁博, 葛安琪, 朱雨彤, 张伶玉, 范洪桥, 刘慧, 胡金辉.补肾活血汤及其拆方对MDA-MB-231乳腺癌细胞增殖、迁移、侵袭能力及其CXCR4表达的影响[J].湖南中医药大学学报英文版,2024,44(10):1781-1786.[Click to copy
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| 补肾活血汤及其拆方对MDA-MB-231乳腺癌细胞增殖、迁移、侵袭能力及其CXCR4表达的影响 |
| 杨争,胡玉蝶,袁博,葛安琪,朱雨彤,张伶玉,范洪桥,刘慧,胡金辉 |
| (湖南中医药大学第一附属医院, 湖南 长沙 410007) |
| 摘要: |
| 目的 探讨补肾活血汤及其拆方组（补肾组、活血组）对人乳腺癌MDA-MB-231细胞增殖、迁移、侵袭能力及CXCR4蛋白表达的影响。方法 分别以补肾活血汤(1.23 g/mL、1.845 g/mL、2.46 g/mL)、补肾中药（0.873 g/mL、1.31 g/mL、1.746 g/mL）、活血中药（0.36 g/mL、0.54 g/mL、0.72 g/mL）汤剂灌胃SD雄性大鼠，制备含药血清。以乳腺癌细胞MDA-MB-231为研究对象，分为对照组，补肾活血汤高、中、低浓度组，补肾中药高、中、低浓度组，活血中药高、中、低浓度组，紫杉醇组。采用MTT法探究上述各组MDA-MB-231细胞增殖活力；细胞划痕试验、Transwell小室侵袭实验检测对照组、补肾活血汤高浓度组、补肾中药高浓度组、活血中药高浓度组、紫杉醇组MDA-MB-231细胞迁移、侵袭能力；Western blot 法检测各组高浓度药物对MDA-MB-231细胞中CXCR4蛋白表达的影响。结果 与对照组比较，补肾活血汤低、中、高浓度，补肾中药中、高浓度，活血中药中、高浓度以及紫杉醇可显著抑制MDA-MB-231细胞增殖活性；补肾活血汤高浓度、活血中药高浓度、补肾中药高浓度和紫杉醇均显著抑制MDA-MB-231细胞迁移、侵袭能力（P<0.05或P<0.01）和CXCR4蛋白表达水平（P<0.05），其中，补肾活血汤高浓度组和紫杉醇组效果更佳（P<0.05）。结论 补肾活血汤可抑制人乳腺癌MDA-MB-231细胞的增殖、侵袭、迁移能力，可能与下调MDA-MB-231细胞表面CXCR4蛋白表达水平相关。 |
| 关键词: 补肾活血汤 三阴性乳腺癌 MDA-MB-231 CXCR4 增殖 迁移 侵袭 |
| DOI：10.3969/j.issn.1674-070X.2024.10.009 |
| Received:December 13, 2023 |
| 基金项目:国家自然科学基金面上项目(82474518);国家自然科学基金青年项目(82405414);湖南省自然科学基金面上项目(2023JJ30470);湖南省自然科学基金联合项目(2023JJ60346);湖南省中药管理局重点项目(C2022020);第五批全国中医临床优秀人才湘中医药科教便函[2022]2号;湖南省卫计委一般课题(D202304139639);湖南省教育厅重点课题(23A0295)。 |
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| Effects of Bushen Huoxue Decoction and its disassembled formulas on proliferation, migration, invasion, and CXCR4 expression of MDA-MB-231 breast cancer cells |
| YANG Zheng, HU Yudie, YUAN Bo, GE Anqi, ZHU Yutong, ZHANG Linyu, FAN Hongqiao, LIU Hui, HU Jinhui |
| (The First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China) |
| Abstract: |
| Objective To investigate the effects of Bushen Huoxue Decoction (BSHXD) and its disassembled groups (Bushen group and Huoxue group) on the proliferation, migration, invasion, and CXCR4 protein expression of human breast cancer MDA-MB-231 cells. Methods BSHXD (1.23 g/mL, 1.845 g/mL, 2.46 g/mL), kidney-tonifying Chinese medicines decoction (0.873 g/mL, 1.31 g/mL, 1.746 g/mL), and blood-circulating Chinese medicines decoction (0.36 g/mL, 0.54 g/mL, 0.72 g/mL) were given by gavage to prepare drug-containing serum. The breast cancer cell MDA-MB-231 was used as the research object and divided into control group, high-, medium-, and low-concentration BSHXD groups, high-, medium-, and low-concentration kidney-tonifying Chinese medicines groups, and high-, medium-, and low-concentration blood-circulating Chinese medicines groups, and paclitaxel group The MTT assay was used to investigate the proliferation activity of MDA-MB-231 cells in the aforementioned groups. Cell scratch test and Transwell chamber invasion test were conducted to determine the migration and invasion abilities of MDA-MB-231 cells in the control group, high-concentration BSHXD group, high-concentration kidney-tonifying Chinese medicines group, high-concentration blood-circulating Chinese medicines group, and paclitaxel group. Western blot was used to examine the effects of high concentration drugs in each group on CXCR4 protein expression in MDA-MB-231 cells. Results Compared with the Control group, Low, Medium, and High concentrations of Bushen Huoxue Tang, Medium and High concentrations of Bushen Chinese herbal medicine, Medium and High concentrations of Huoxue Chinese herbal medicine, and Paclitaxel can significantly inhibit the proliferation activity of MDA-MB-231 cells; The high concentration of Bushen Huoxue Tang, High concentration of Huoxue traditional Chinese medicine, High concentration of Bushen traditional Chinese medicine, and Paclitaxel all significantly inhibited the migration and invasion ability of MDA-MB-231 cells (P<0.05 or P<0.01) and the expression level of CXCR4 protein (P<0.05). Among them, the high concentration group of Bushen Huoxue decoction and the paclitaxel group had better effects (P<0.05). Conclusion BSHXD can inhibit the proliferation, invasion, and migration abilities of human breast cancer MDA-MB-231 cells, which may be related to the down-regulation of CXCR4 gene expression on the surface of MDA-MB-231 cells. |
| Key words: Bushen Huoxue Decoction triple negative breast cancer MDA-MB-231 CXCR4 proliferation migration invasion |
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