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闫艳, 葛雨竹, 张小倩, 黄珊, 赵权, 王婕, 杨晓帆.基于指纹图谱的化学模式识别法和多指标测定评价枸杞不同部位的差异性[J].湖南中医药大学学报英文版,2024,44(7):1193-1202.[Click to copy
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| 基于指纹图谱的化学模式识别法和多指标测定评价枸杞不同部位的差异性 |
| 闫艳,葛雨竹,张小倩,黄珊,赵权,王婕,杨晓帆 |
| (亳州学院中药学院, 安徽 亳州 236800;中科科博检测有限公司, 安徽 亳州 236800) |
| 摘要: |
| 目的 研究枸杞植株不同部位中主要化学成分的整体信息和含量差异，综合评价枸杞不同部位的质量和合理应用。方法 以3个主产区的枸杞子、枸杞叶和地骨皮药材为研究对象，建立HPLC指纹图谱，采用中药色谱指纹图谱相似度评价系统进行相似度评价；结合SIMCA 14.1软件进行主成分分析等；采用分光光度比色法测定枸杞多糖和总黄酮含量；并利用HPLC方法对甜菜碱进行含量测定。结果 枸杞不同部位化学成分的种类和含量均存在明显差异，叶和果实含有更丰富的化学成分，枸杞多糖、总黄酮和甜菜碱的含量均为枸杞叶＞枸杞子＞地骨皮。结论 枸杞叶含有更丰富的营养成分，其药理功能和营养价值可能优于枸杞子。因此,可考虑多产地开发枸杞叶作为枸杞药材来源之一，以促进枸杞资源的多途径、多层次开发利用。 |
| 关键词: 枸杞子 枸杞叶 地骨皮 HPLC指纹图谱 化学计量学 多糖 黄酮 甜菜碱 |
| DOI：10.3969/j.issn.1674-070X.2024.07.007 |
| Received:December 28, 2023 |
| 基金项目:安徽省高校自然科学研究重点项目（2023AH052272，KJ2021A1149）；亳州学院校级质量工程项目（2023XJXM134）。 |
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| Evaluating the differences in different parts of Lycium barbarum L. by fingerprint-based chemical pattern recognition method and multi-index determination |
| YAN Yan, GE Yuzhu, ZHANG Xiaoqian, HUANG Shan, ZHAO Quan, WANG Jie, Yang Xiaofan |
| (College of Chinese Medicine, Bozhou University, Bozhou, Anhui 236800, China;Zhongke Kebo Testing Co., Ltd, Bozhou, Anhui 236800, China) |
| Abstract: |
| Objective To study the overall information and content differences of the main chemical components in different parts of Lycium barbarum L., and comprehensively evaluate the quality and rational application of different parts of Lycium barbarum L.. Methods This study focused on Gouqizi (Lycii Fructus), Gouqiye (Lycii leafy), and Digupi (Lycii Cortex) from three major producing regions as the subjects of investigation. HPLC fingerprints were established for these samples, and similarity evaluations were performed using the chromatographic fingerprint similarity evaluation system for Chinese medicines. Clustering and principal component analysis were conducted using SIMCA 14.1 software. The polysaccharide and total flavonoid contents were determined through spectrophotometric colorimetric methods, while the betaine content was quantified using the HPLC method. Results Significant differences were observed in the types and contents of chemical components among different parts of Lycium barbarum L. Gouqiye (Lycii leafy) and Gouqizi (Lycii Fructus) were found to contain richer chemical components. The contents of polysaccharides, total flavonoids, and betaine were ranked in the order of Gouqiye (Lycii leafy) > Gouqizi (Lycii Fructus) > Digupi (Lycii Cortex). Conclusion Gouqiye (Lycii leafy) are richer in nutrients, the pharmacological functions and nutritional value may be superior to those of Gouqizi (Lycii Fructus). Therefore, the multi-origin development of Gouqiye (Lycii leafy) as high-quality Lycium barbarum medicinal herbs can be considered for the multi-path and multi-level development and utilization of Lycium barbarum L. resources. |
| Key words: Lycii Fructus Lycii leafy Lycii Cortex HPLC fingerprint chemometrics polysaccharides flavonoids betaine |
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