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赖桂花,谭金曲,李萌萌,付成效,曾亚华,王婷,王娟,廖念,周君,屈萌艰.血必净注射液对LPS诱导的急性肺损伤大鼠炎症反应及细胞凋亡的影响[J].湖南中医药大学学报英文版,2024,44(4):538-544.[Click to copy
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| 血必净注射液对LPS诱导的急性肺损伤大鼠炎症反应及细胞凋亡的影响 |
| 赖桂花,谭金曲,李萌萌,付成效,曾亚华,王婷,王娟,廖念,周君,屈萌艰 |
| (南华大学附属第一医院康复医学中心, 湖南 衡阳 421001;南华大学附属第一医院康复医学科, 湖南 衡阳 421001;南华大学附属第一医院针灸/康复医学实验室, 湖南 衡阳 421001;南华大学附属第一医院药学部, 湖南 衡阳 421001) |
| 摘要: |
| 目的 观察血必净注射液对脂多糖（lipopolysaccharide，LPS）诱导的急性肺损伤大鼠炎症反应及细胞凋亡的影响。方法 将30只雄性SD大鼠随机分为正常组（10只）和造模组（20只），造模组造模后（尾静脉注射LPS）随机分为模型组（10只）和血必净注射液组（10只）。采用肺组织湿重/干重评估肺组织水肿程度，ELISA检测血清炎症因子IL-6、IL-1β、IL-4和IL-18的水平，HE染色观察大鼠肺组织病理损伤情况，TUNEL染色观察肺组织细胞凋亡情况，RT-PCR和Western blot检测凋亡因子胱天蛋白酶-3（Caspase-3）和胱天蛋白酶-8（Caspase-8）的mRNA含量和蛋白水平。结果 与正常组比，模型组大鼠肺组织湿重/干重升高（P＜0.01），IL-6、IL-1β、IL-4和IL-18升高（P＜0.001），肺组织出现损伤和炎症浸润，细胞凋亡率升高（P＜0.001），Caspase-3和Caspase-8的mRNA、蛋白表达升高（P＜0.01或P＜0.001）。与模型组比，血必净注射液组大鼠肺组织湿重/干重下降（P＜0.05），IL-6、IL-1β、IL-4和IL-18降低（P＜0.05或P＜0.01），肺组织损伤和炎症浸润情况明显改善，细胞凋亡率下降（P＜0.001），Caspase-3和Caspase-8的mRNA和蛋白表达降低（P＜0.01或P＜0.001）。结论 血必净注射液可改善急性肺损伤大鼠的肺组织损伤和炎症反应，其机制可能与抑制细胞凋亡有关。 |
| 关键词: 急性肺损伤 血必净注射液 炎症反应 细胞凋亡 胱天蛋白酶-3 胱天蛋白酶-8 |
| DOI：10.3969/j.issn.1674－070X.2024.04.002 |
| Received:January 21, 2024 |
| 基金项目:湖南省自然科学基金青年项目（2023JJ40595）；湖南省卫生健康委员会项目（202103100165）。 |
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| Effects of Xuebijing Injection on inflammatory response and cell apoptosis in rats with LPS-induced acute lung injury |
| LAI Guihua,TAN Jinqu,LI Mengmeng,FU Chengxiao,ZENG Yahua,WANG Ting,WANG Juan,LIAO Nian,ZHOU Jun,QU Mengjian |
| (Rehabilitation Medicine Center, the First Hospital of University of South China, Hengyang, Hunan 421001, China;Department of Rehabilitation, the First Hospital of University of South China, Hengyang, Hunan 421001, China;Acupuncture&Rehabilitation Laboratory, the First Hospital of University of South China, Hengyang, Hunan 421001, China;Department of Pharmacy, the First Hospital of University of South China, Hengyang, Hunan 421001, China) |
| Abstract: |
| Objective To observe the effects of Xuebijing Injection (XBJI) on inflammatory response and cell apoptosis in rats with lipopolysaccharide (LPS)-induced acute lung injury (ALI).Methods A total of 30 male SD rats were randomized into normal group (n=10) and modeling group (n=20) which was subdivided randomly into model group (n=10) and XBJI group (n=10) after modeling (injection of LPS into the tail vein).The degree of pulmonary edema was evaluated using the wet/dry weight ratio (W/D) of the lung tissue,the serum levels of inflammatory factors IL-6,IL-1β,IL-4,and IL-18 were determined by ELISA,the pathological damage of rat lung tissue was observed by HE staining,the condition of cell apoptosis was observed by TUNEL staining,and the mRNA content and protein levels of apoptotic factors Caspase-3 and Caspase-8 were measured by RT-PCR and Western blot.Results Compared with the normal group,the model group showed an increase in the rat lung tissue W/D (P＜0.01),levels of IL-6,IL-1β,IL-4,and IL-18(P＜0.001),cell apoptosis rate (P＜0.001),as well as the mRNA and protein expressions of Caspase-3 and Caspase-8(P＜0.01 or P＜0.001);besides,the lung tissue was damaged with inflammatory cell infiltration.Compared with the model group,the XBJI group exhibited a decrease in the lung tissue W/D (P＜0.05),levels of IL-6,IL-1β,IL-4,and IL-18(P＜0.05 or P＜0.01),cell apoptosis rate (P＜0.001),as well as the mRNA and protein expressions of Caspase-3 and Caspase-8(P＜0.01 or P＜0.001);additionally,the damage to the lung tissue and inflammatory cell infiltration were significantly alleviated.Conclusion XBJI can relieve the lung tissue injury and inflammatory response in ALI rats,the mechanism of which may be related to inhibiting cell apoptosis. |
| Key words: acute lung injury Xuebijing Injection inflammatory response cell apoptosis Caspase-3 Caspase-8 |
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