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袁汉文,李云耀,梁玲,吕梦颖,王炜.基于HPLC和LC-Orbitrap-MS的栀子化学成分定性与定量研究[J].湖南中医药大学学报英文版,2023,43(12):2199-2205.[Click to copy
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| 基于HPLC和LC-Orbitrap-MS的栀子化学成分定性与定量研究 |
| 袁汉文,李云耀,梁玲,吕梦颖,王炜 |
| (湖南中医药大学中医药民族医药国际联合实验室, 湖南 长沙 410208;湖南交通工程学院, 湖南 衡阳 421001) |
| 摘要: |
| 目的 对栀子中的化学成分进行定性和定量研究，为栀子的质量控制和评价提供参考。方法 采用HPLC双波长法对栀子中的主要成分进行定性与定量分析，利用LC-Orbitrap-MS，结合mzCloud和mzVault数据库对栀子中的化学成分进行鉴定。结果 建立了栀子HPLC特征图谱，并通过与对照品溶液进行比较指认了11个主要成分，对其中6个主要成分进行了定量，通过LC-Orbitrap-MS鉴定了31个成分。结论 建立的HPLC定量方法稳定可靠，可用于栀子的质量控制，LC-Orbitrap-MS技术可实现对栀子快速的全成分分析，为栀子的进一步开发利用提供了科学依据。 |
| 关键词: 栀子 HPLC LC-Orbitrap-MS 化学成分 质量控制 |
| DOI：10.3969/j.issn.1674-070X.2023.12.009 |
| Received:May 08, 2023 |
| 基金项目:国家重点研发计划“中医药现代化研究”专项“中药国际标准示范研究”（2018YFC1707903）；湖南省自然科学基金青年项目（2022JJ40318）。 |
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| Qualitative and quantitative study on chemical constituents of Zhizi(Gardeniae Fructus) based on HPLC and LC-Orbitrap-MS |
| YUAN Hanwen,LI Yunyao,LIANG Ling,LYU Mengying,WANG Wei |
| (TCM and Ethnomedicine Innovation & Development International Laboratory, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Hunan Institute of Traffic Engineering, Hengyang, Hunan 421001, China) |
| Abstract: |
| Objective To qualitatively and quantitatively study the chemical constituents of Zhizi(Gardeniae Fructus), so as to provide references for its quality control and evaluation.Methods The main components of Zhizi(Gardeniae Fructus) were qualitatively and quantitatively analyzed by HPLC dual-wavelength method, and the chemical constituents in Zhizi(Gardeniae Fructus) were identified by LC-Orbitrap-MS combined with mzCloud and mzVault databases.Results The HPLC characteristic chromatogram of Zhizi(Gardeniae Fructus) was established, and 11 main components were identified by comparing with reference solution. Six of them were quantified, and 31 components were identified by LC-Orbitrap-MS.Conclusion The established HPLC quantitative method is stable and reliable, and can be used for the quality control of Zhizi(Gardeniae Fructus). Moreover, LC-Orbitrap-MS technology can achieve rapid full-component analysis of Zhizi(Gardeniae Fructus), providing a scientific basis for its further development and utilization. |
| Key words: Gardeniae Fructus HPLC LC-Orbitrap-MS chemical constituents quality control |
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