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叶子丰,戎宽,匡建军,仇湘中,许辉.补肝健膝方对膝骨关节炎模型兔COMP、CTX-Ⅱ及炎性指标的影响[J].湖南中医药大学学报英文版,2023,43(12):2157-2162.[Click to copy
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| 补肝健膝方对膝骨关节炎模型兔COMP、CTX-Ⅱ及炎性指标的影响 |
| 叶子丰,戎宽,匡建军,仇湘中,许辉 |
| (湖南中医药大学, 湖南 长沙 410208;湖南省中医药研究院附属医院, 湖南 长沙 410006;湖南省中医药研究院, 湖南 长沙 410006) |
| 摘要: |
| 目的 通过研究补肝健膝方对膝骨关节炎（knee osteoarthritis，KOA）模型兔血清、关节腔积液中白细胞介素-1（interleukin-1，IL-1）、肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）水平以及血清中软骨寡聚基质蛋白（cartilage oligomeric matrix protein，COMP）和Ⅱ型胶原C端肽（C-telopeptide of typeⅡcollagen，CTX-Ⅱ）含量的影响，进一步揭示补肝健膝方对KOA的作用机制。方法 采用木瓜蛋白酶关节注射法制作兔KOA模型。随机分为空白对照组、模型对照组、独活寄生汤组及补肝健膝方低、中、高剂量组，每组5只。造模4周后，空白对照组和模型对照组每日灌胃生理盐水1次；独活寄生汤组每日以独活寄生汤煎剂2.895 g/（kg·d）灌胃1次；补肝健膝方低、中、高剂量组每日以补肝健膝方煎液[1.962、3.924、7.848 g/（kg·d）]灌胃1次。治疗4周后，采用HE染色观察膝关节软骨的病理改变，Pelletier评分评估软骨损伤情况，ELISA检测血清、关节腔积液IL-1、TNF-α水平以及血清COMP、CTX-Ⅱ含量。结果 与空白对照组相比，模型对照组软骨关节表面欠光滑，软骨局部可见黏液样变，软骨及深层细胞数目显著降低；Pelletier评分显著升高（P<0.05）；血清及关节腔积液中的IL-1、TNF-α含量及血清中COMP、CTX-Ⅱ含量均升高（P<0.05）。与模型对照组相比，独活寄生汤组、补肝健膝方各剂量组软骨表面层次分布更为清晰，潮线结构完整；Pelletier评分降低（P<0.05）；血清、关节腔积液IL-1、TNF-α水平以及血清中COMP和CTX-Ⅱ含量均降低（P<0.05）。与独活寄生汤组比较，补肝健膝方各剂量组软骨表面层次分布更为清晰，潮线结构更为完整；Pelletier评分升高（P<0.05）；血清及关节腔积液IL-1、TNF-α及血清中COMP、CTX-Ⅱ含量降低（P<0.05）。结论 补肝健膝方可有效改善KOA兔骨微结构、调节其膝关节滑膜细胞活性，可能与抑制血清、关节腔积液IL-1、TNF-ɑ水平以及血清中COMP和CTX-Ⅱ含量有关。 |
| 关键词: 补肝健膝方 独活寄生汤 膝骨关节炎 软骨寡聚基质蛋白 Ⅱ型胶原C端肽 炎性指标 |
| DOI：10.3969/j.issn.1674-070X.2023.12.003 |
| Received:April 25, 2023 |
| 基金项目:国家中医药管理局“全国名老中医药专家仇湘中传承工作室建设项目”（国中医药人教[2016]42号）；湖南省科学技术厅临床创新引导项目（2020SK51002）；长沙市科技局项目（kq2004046）；湖南省自然科学基金面上项目（2022JJ30359）；湖南省中医药管理局重点课题项目（C2022018）。 |
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| Effects of Bugan Jianxi Formula on COMP, CTX-Ⅱ, and inflammatory indexes in knee osteoarthritis model rabbits |
| YE Zifeng,RONG Kuan,KUANG Jianjun,QIU Xiangzhong,Xu Hui |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The Hospital of Hunan Academy of Chinese Medicine, Changsha, Hunan 410006, China) |
| Abstract: |
| Objective To study the effects of Bugan Jianxi Formula(BGJXF) on levels of serum, interleukin-1(IL-1), and tumor necrosis factor-α level(TNF-α), and the content of cartilage oligomeric matrix protein(COMP) and C-telopeptide of type Ⅱ collagen(CTX-Ⅱ) in the serum, and to reveal the mechanism of action of BGJXF on knee osteoarthritis(KOA).Methods Rabbit KOA model was constructed through papain joint injection, and they were randomly divided into blank control group, model control group, Duhuo Jisheng Decoction(DHJSD) group, and low-, medium-, and high-dose BGJXF groups, with five rabbits in each group. After four weeks of modeling, the blank control and the model control groups received normal saline one time per day; DHJSD group received gastric administration of DHJSD 2.895 g/(kg·d) one time per day; low-, medium-, and high-dose BGJXF groups received gastric administration of BGJXF decoction [1.962, 3.924, 7.848 g/(kg·d)] once per day. After four weeks, the pathological changes in the cartilage of the knee were observed by HE staining; cartilage damage was assessed by Pelletier score; IL-1 and TNF-α levels of the serum and the articular cavity effusion, and COMP and CTX-Ⅱ content of the serum was measured by ELISA.Results Compared with the blank control group, the cartilage articular surface of the model control group was not smooth, mucoid changes could be seen locally in the cartilage, and the number of cartilage and deep cells was significantly reduced; the Pelletier score was significantly elevated(P<0.05); the content of IL-1 and TNF-α in the serum and articular cavity effusion as well as the content of COMP and CTX-Ⅱ in the serum were all elevated(P<0.05). Compared with the model control group, the cartilage surface levels were more clearly distributed and the tideline structure was intact in the DHJSD group and each dose group of BGJXF; the Pelletier score was reduced(P<0.05); the levels of IL-1 and TNF-α in serum and articular cavity effusion as well as the content of COMP and CTX-Ⅱin serum were all reduced(P<0.05). Compared with the DHJSD group, the cartilage surface level distribution of each dose group of BGJXF was clearer, and the tideline structure was more complete; the Pelletier score increased(P<0.05); the content of IL-1 and TNF-α in serum and articular cavity fluid, and the content of COMP and CTX-Ⅱ in serum decreased(P<0.05).Conclusion BGJXF can effectively improve the bone microstructure and regulate the synovial cell activity of knee articular in rabbits with KOA, which may be related to the inhibition of IL-1 and TNF-α levels in the serum and articular cavity fluid as well as the content of COMP and CTX-Ⅱ in serum. |
| Key words: Bugan Jianxi Formula Duhuo Jisheng Decoction knee osteoarthritis cartilage oligo matrix protein C-telopeptide of type Ⅱ collagen inflammatory indexes |
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