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蔡楚豪,展嘉文,韩涛,谢瑞,张伟业,王戈文,朱立国.补肾活血方对内源性“筋伤”腰椎失稳兔模型的影响[J].湖南中医药大学学报英文版,2023,43(10):1803-1808.[Click to copy
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| 补肾活血方对内源性“筋伤”腰椎失稳兔模型的影响 |
| 蔡楚豪,展嘉文,韩涛,谢瑞,张伟业,王戈文,朱立国 |
| (北京中医药大学, 北京 100015;中国中医科学院望京医院, 北京 100015;北京中医医院顺义医院, 北京 101300) |
| 摘要: |
| 目的 探究补肾活血方对内源性“筋伤”腰椎失稳兔模型的影响。方法 22只健康新西兰兔行韧带损伤型“筋伤”腰椎失稳动物模型手术，造模后随机分为补肾活血方组和模型组，每组11只，另取11只健康新西兰兔为假手术组。补肾活血方组予补肾活血方中药溶液（生药3.597 g·kg-1·d-1灌胃，每日给药1次），假手术组、模型组灌胃等体积的生理盐水，均灌胃8周。于造模前及造模后4、8周行X线透视，进行椎体水平位移、椎间高度指数（disc height index, DHI）测算；取新西兰兔完整腰椎椎间盘，行HE染色以及TUNEL染色，观察各组病理情况。结果 与假手术组比较，模型组椎体水平位移增加（P＜0.05）、DHI明显降低（P＜0.05）、椎间盘组织细胞凋亡率升高（P＜0.05）。与模型组比较，补肾活血方组第8周椎体水平位移减少（P＜0.05）、DHI升高（P＜0.05），椎间盘组织细胞凋亡率在第4、第8周均降低（P＜0.05）。结论 补肾活血方可能通过减小腰椎椎体水平位移，提高椎体的DHI，降低椎间盘组织细胞的凋亡率，对韧带损伤型“筋伤”腰椎失稳动物模型起治疗作用。 |
| 关键词: 补肾活血方 退行性腰椎失稳 椎体水平位移 椎间高度指数 新西兰兔 |
| DOI：10.3969/j.issn.1674-070X.2023.10.008 |
| Received:May 24, 2023 |
| 基金项目:国家自然科学基金重点项目（81930118）。 |
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| Effects of Bushen Huoxue Formula on rabbit model with lumbar instability induced by endogenous "sinew injury" |
| CAI Chuhao,ZHAN Jiawen,HAN Tao,XIE Rui,ZHANG Weiye,WANG Gewen,ZHU Liguo |
| (Beijing University of Chinese Medicine, Beijing 100015, China;Wangjing Hospital, China Academy of Chinese Medical Sciences, Beijing 100015, China;Shunyi Hospital, Beijing Hospital of Chinese Medicine, Beijing 101300, China) |
| Abstract: |
| Objective To explore the effects of Bushen Huoxue Formula (BSHXF) on rabbit model with lumbar instability induced by endogenous "sinew injury". Methods A total of 22 healthy New Zealand rabbits were undergone ligamentous injury surgery to create an animal model with lumbar instability induced by "sinew injury". After modeling, the rabbits were randomly divided into BSHXF group and model group, with 11 rabbits in each. Another 11 healthy New Zealand rabbits were used as sham-operated group. BSHXF group was given medicinal solution of BSHXF (crude drug 3.597 g·kg-1·d-1 by gavage, once daily), while sham-operated group and model group were given the same volume of normal saline by gavage. All groups were treated for 8 weeks. X-ray examination was performed before and 4 and 8 weeks after modeling to measure the horizontal displacement of the vertebral body and the disc height index (DHI); the intact lumbar intervertebral discs taken from the New Zealand rabbits were stained with HE and TUNEL, through which the pathological conditions of each group were observed. Results Compared with sham-operated group, model group showed an increase in horizontal displacement of vertebral body (P＜0.05) and a significant decrease in DHI (P＜0.05), and higher apoptosis rate of intervertebral disc tissue (P＜0.05). Compared with model group, BSHXF group showed a decrease in horizontal displacement of vertebral body (P＜0.05) and an increase in DHI (P＜0.05) at the 8th week, and lower apoptosis rate of intervertebral disc tissue at the 4th and 8th week (P＜0.05). Conclusion BSHXF may have a curative effect on the rabbit model with lumbar instability induced by "sinew injury" by reducing the horizontal displacement of the lumbar vertebral body, increasing the DHI of the vertebra, and decreasing the apoptosis rate of intervertebral disc histocyte. |
| Key words: Bushen Huoxue Formula degenerative lumbar spinal instability horizontal displacement of the lumbar vertebral body disc height index New Zealand rabbit |
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