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李冬,董明国,房志科,黄林石,陈继英.基于线粒体凋亡通路探讨防己诺林碱对前列腺癌PC3细胞的生物学行为的影响[J].湖南中医药大学学报英文版,2023,43(7):1194-1200.[Click to copy
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| 基于线粒体凋亡通路探讨防己诺林碱对前列腺癌PC3细胞的生物学行为的影响 |
| 李冬,董明国,房志科,黄林石,陈继英 |
| (广州中医药大学东莞医院, 广东 东莞 523005) |
| 摘要: |
| 目的 基于线粒体凋亡通路探究防己诺林碱（fangchinoline，FAN）对前列腺癌PC3细胞生物学行为的影响。方法 将前列腺癌PC3细胞按照FAN处理浓度分为4组，分别为FAN 0 μmol/L组、FAN 5 μmol/L组、FAN 10 μmol/L组和FAN 20 μmol/L组，各组依次采用FAN 0 μmol/L （DMSO替代）、5 μmol/L、10 μmol/L和20 μmol/L进行处理，孵育48 h后进行实验。CCK-8法检测PC3细胞增殖，平板克隆法检测PC3细胞克隆形成能力，流式细胞仪检测PC3细胞凋亡，Transwell实验检测PC3细胞侵袭，划痕实验检测PC3细胞迁移，Western blot检测PC3细胞自噬相关蛋白和线粒体凋亡通路蛋白。结果 与FAN 0 μmol/L组相比，FAN 5 μmol/L组、FAN 10 μmol/L组和FAN 20 μmol/L组的PC3细胞增殖能力、克隆形成能力、侵袭和迁移能力明显降低（P<0.001），凋亡率明显升高（P<0.001）；与FAN 5 μmol/L组和FAN 10 μmol/L组相比，FAN 20 μmol/L组的PC3细胞增殖能力、克隆形成能力、侵袭和迁移能力明显降低（P<0.001）；与FAN 0 μmol/L组相比，FAN 5 μmol/L组、FAN 10 μmol/L组和FAN 20 μmol/L组的PC3细胞中P62和Bcl-2蛋白表达明显降低（P<0.001），LC3B、Bax和Caspase-3蛋白表达明显升高（P<0.001）。结论 FAN可能通过线粒体凋亡通路抑制细胞增殖、侵袭和迁移，促进其自噬和凋亡，并呈现剂量依赖性。 |
| 关键词: 前列腺癌 防己诺林碱 线粒体凋亡 PC3细胞 增殖 自噬 |
| DOI：10.3969/j.issn.1674-070X.2023.07.007 |
| Received:November 30, 2022 |
| 基金项目:粤莞联合基金青年基金项目（2020A1515111085）。 |
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| Effects of fangchinoline on biological behavior of prostate cancer PC3 cells based on mitochondrial apoptosis pathway |
| LI Dong,DONG Mingguo,FANG Zhike,HUANG Linshi,CHEN Jiying |
| (Dongguan Hospital of Guangzhou University of Chinese Medicine, Dongguan, Guangdong 523005, China) |
| Abstract: |
| Objective To explore the effects of fangchinoline (FAN) on the biological behavior of prostate cancer PC3 cells based on mitochondrial apoptosis pathway. Methods Prostate cancer PC3 cells were divided into 4 groups according to FAN treatment concentrations, namely FAN 0 μmol/L, 5 μmol/L, 10 μmol/L and 20 μmol/L groups, which were treated with FAN 0 μmol/L (DMSO replacement), 5 μmol/L, 10 μmol/L and 20 μmol/L, respectively, then the experiment was carried out after 48 h incubation. The proliferation of PC3 cells was measured by CCK-8 method, the cloning ability of PC3 cells was determined by plate cloning method, the apoptosis of PC3 cells was detected by flow cytometry, the invasion of PC3 cells was checked by Transwell assay, the migration of PC3 cells was examined by scratch assay, and the autophagy related proteins and mitochondrial apoptosis pathway proteins of PC3 cells were measured by Western blot. Results Compared with FAN 0 μmol/L group, the proliferation, clone formation, invasion and migration of PC3 cells in FAN 5 μmol/L, 10 μmol/L and 20 μmol/L groups were significantly lower (P<0.001), and the apoptosis rate was significantly higher (P<0.001). Compared with FAN 5 μmol/L and FAN 10 μmol/L groups, the proliferation, clone formation, invasion and migration of PC3 cells in FAN 20 μmol/L group were significantly lower (P<0.001). Compared with FAN 0 μmol/L group, the expressions of P62 and Bcl-2 in PC3 cells in FAN 5 μmol/L, 10 μmol/L and 20 μmol/L groups were significantly lower (P<0.001), and the expressions of LC3B, Bax and Caspase-3 proteins were significantly higher (P<0.001). Conclusion FAN may inhibit cell proliferation, invasion and migration through mitochondrial apoptosis pathway, and promote autophagy and apoptosis in a dose-dependent manner. |
| Key words: prostate cancer fangchinoline mitochondrial apoptosis PC3 cells proliferation autophagy |
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