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刘惠娟,欧阳里知,李芊,彭涵,刘红华,葛君芸,罗坚,常小荣,刘迈兰,汪厚莲.基于Wnt/β-catenin通路探讨隔药饼灸对高脂血症兔调脂机制的影响[J].湖南中医药大学学报英文版,2023,43(6):1098-1104.[Click to copy
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| 基于Wnt/β-catenin通路探讨隔药饼灸对高脂血症兔调脂机制的影响 |
| 刘惠娟,欧阳里知,李芊,彭涵,刘红华,葛君芸,罗坚,常小荣,刘迈兰,汪厚莲 |
| (湖南中医药高等专科学校, 湖南 株洲 412012;湖南中医药大学, 湖南 长沙 410208) |
| 摘要: |
| 目的 观察隔药饼灸对高脂血症兔肝组织Wnt1、β-卷曲蛋白（β-catenin）、散乱蛋白-1（dishevelled-1，dvl-1) 、固醇调节元件结合蛋白1c(sterol regulatory element-binding protein1c, SREBP1c)的mRNA表达量的影响，探讨隔药饼灸调脂的作用机制。方法 将48只新西兰兔随机分为正常组、模型组、隔药饼灸组和阿托伐他汀组，每组12只。正常组采用普通饲料喂养，其余各组采用高脂饲养法喂养8周，待造模成功后，除正常组、模型组外，隔药饼灸组选取两组穴位交替施灸（Ⅰ组穴位为巨阙、天枢、丰隆；Ⅱ组穴位为心俞、肝俞、脾俞），阿托伐他汀组将阿托伐他汀钙片碾成粉末后拌入第一口饲料喂食，每日1次，连续8周。测定各组兔血清总胆固醇（total cholesterol, TC）、甘油三酯（glycerin trilaurate, TG）、低密度脂蛋白胆固醇（low density lipoprotein cholesterol, LDL-C）含量，RT-PCR法测定各组兔Wnt1、β-catenin、dvl-1、SREBP1c的mRNA表达量，油红O染色观察肝组织脂质沉积情况。结果 （1）血脂结果：与正常组相比，模型组血清TC、TG、LDL-C上升（P<0.001）；与模型组相比，隔药饼灸组和阿托伐他汀组TC、TG、LDL-C下降（P<0.01，P<0.001）；隔药饼灸组与阿托伐他汀组相比，TC、TG、LDL-C差异无统计学意义（P>0.05）。（2）RT-PCR结果：与正常组相比，模型组肝组织Wnt1、β-catenin、dvl-1、SREBP1c的mRNA表达量上升（P<0.001）；与模型组相比，隔药饼灸组和阿托伐他汀组肝组织Wnt1、β-catenin、dvl-1、SREBP1c的mRNA表达量均下降（P<0.01，P<0.001）；隔药饼灸组与阿托伐他汀组肝组织Wnt1、β-catenin、dvl-1、SREBP1c的mRNA表达差异无统计学意义（P>0.05）。（3）脂质沉积结果：与正常组相比，模型组、隔药饼灸组、阿托伐他汀组脂质沉积均有增加，模型组增加更明显；与模型组相比，隔药饼灸组、阿托伐他汀组脂质沉积明显减少；隔药饼灸组与阿托伐他汀组无明显差异。结论 隔药饼灸可能通过抑制Wnt/β-catenin通路，下调高脂血症兔SREBP1c含量，发挥调脂作用。 |
| 关键词: 隔药饼灸|高脂血症|血脂|Wnt1|β-卷曲蛋白|散乱蛋白-1|固醇调节元件结合蛋白 1c |
| DOI：10.3969/j.issn.1674－070X.２０23.06.022 |
| Received:September 19, 2022 |
| 基金项目:国家自然科学基金项目（82074559）；湖南省研究生科研创新项目（CX20200781）；长沙市杰出创新青年培养计划项目（kq1905036）；湖南省教育厅优秀青年基金项目（19B435，19B428）；湖南中医药大学第一附属医院湖南省院士专家工作站（石学敏）开放基金（2019YSZJJ）；湖南省中医药科研计划课题项目（201964）。 |
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| Effects of medicinal cake-separated moxibustion on the mechanism of regulating lipid in rabbits with hyperlipidemia based on Wnt/β-catenin pathway |
| LIU Huijuan,OUYANG Lizhi,LI Qian,PENG Han,LIU Honghua,GE Junyun,LUO Jian,CHANG Xiaorong,LIU Mailan,WANG Houlian |
| (Hunan College of Chinese Medicine, Zhuzhou, Hunan 412012, China;Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To observe the effects of medicinal cake-separated moxibustion on Wnt1, β-catenin, dishevelled-1 (dvl-1), and sterol regulatory element-binding protein1c (SREBP1c) in liver tissue of hyperlipidemia rabbits and to explore the mechanism of action of medicinal cake-separated moxibustion on lipid regulating. Methods A total of 48 New Zealand rabbits were randomly divided into normal group, model group, medicinal cake-separated moxibustion group, and atorvastatin group, with 12 rabbits in each group. The normal group was fed with normal diet, while the other groups were fed with high-fat diet for 8 weeks. After successful modeling, except normal group and model group, two groups of acupoints in medicinal cake-separated moxibustion group were selected for alternating moxibustion [group Ⅰ: 'Juque' (RN14), 'Tianshu' (ST25), and 'Fenglong' (ST40); group Ⅱ: 'Xinshu' (BL15), 'Ganshu' (BL18), and 'Pishu' (BL20)]; in atorvastatin group, atorvastatin calcium tablets were ground into powder and mixed into the first feed, once a day for 8 consecutive weeks in both groups. The content of total cholesterol (TC), glycerin trilaurate (TG), and low density lipoprotein cholesterol (LDL-C) in serum of rabbits in each group were determined. The mRNA expression levels of Wnt1, β-catenin, dvl-1 and SREBP1c were determined by RT-PCR. Lipid deposition in liver tissue was observed by oil red "O" staining. Results (1) Lipid results: Compared with normal group, serum TC, TG, and LDL-C in model group increased (P<0.001); compared with model group, TC, TG, and LDL-C in medicinal cake-separated moxibustion group and atorvastatin group decreased (P<0.01, P<0.001); compared with atorvastatin group, there was no significant statistical difference in TC, TG, and LDL-C in medicinal cake-separated moxibustion group (P＞0.05). (2) RT-PCR results: Compared with normal group, mRNA expression levels of Wnt1, β-catenin, dvl-1, and SREBP1c in liver tissue in model group increased (P<0.001); compared with model group, mRNA expression levels of Wnt1, β-catenin, dvl-1, and SREBP1c in liver tissue in both medicinal cake-separated moxibustion group and atorvastatin group decreased (P<0.01，P<0.001). There was no significant statistical difference in mRNA expression levels of Wnt1, β-catenin, dvl-1, and SREBP1c in liver tissue between medicinal cake-separated moxibustion group and atorvastatin group (P＞0.05). (3) Lipid deposition results: Compared with normal group, lipid deposition in model group, medicinal cake-separated moxibustion group, and atorvastatin group increased, with more significant increase in model group; compared with model group, lipid deposition in medicinal cake-separated moxibustion group and atorvastatin group reduced significantly, and there was no significant difference between medicinal cake-separated moxibustion group and atorvastatin group. Conclusion By inhibting Wnt/β-catenin pathway, medicinal cake-separated moxibustion can down-regulate the content of SREBP1c and play the role of lipid regulation. |
| Key words: medicinal cake-separated moxibustion|hyperlipidemia|blood lipid|Wnt1|β-catenin|dishevelled-1|sterol regulatory element-binding protein1c |
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