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汤文娟,夏旭婷,刘富林.枳术丸对脾虚证慢传输型便秘小鼠结肠黏膜AQP3、AQP9的影响[J].湖南中医药大学学报英文版,2023,43(6):999-1005.[Click to copy
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| 枳术丸对脾虚证慢传输型便秘小鼠结肠黏膜AQP3、AQP9的影响 |
| 汤文娟,夏旭婷,刘富林 |
| (湖南中医药大学研究生院, 湖南 长沙 410208;湖南中医药大学中医学院, 湖南 长沙 410208) |
| 摘要: |
| 目的 观察枳术丸对脾虚证慢传输型便秘(slow transit constipation, STC）小鼠结肠AQP3、AQP9表达的影响，探讨枳术丸改善STC症状的可能作用机制。方法 将50只SPF级昆明小鼠随机分成正常组（15只）和造模组（35只），造模组采用复合因素法（番泻叶+限水+控制饮食）建立脾虚证STC小鼠模型，造模成功后造模组小鼠按体质量随机分为模型组（10只）、枳术丸组（10只）、莫沙必利组（10只）。连续给药7 d后，检测各组小鼠的粪便含水量、肠道推进率、血清木糖含量及结肠黏膜组织病理学特征；免疫组化及Western blot法检测AQP3、AQP9蛋白表达。结果 与正常组相比，模型组小鼠粪便含水量显著减少，肠道推进率显著降低，血清木糖含量显著降低，小鼠结肠黏膜AQP3表达显著增多，AQP9表达显著减少（P<0.01）；与模型组相比，枳术丸组小鼠粪便含水量显著增加（P<0.05），肠道推进率显著升高（P<0.01），血清木糖含量显著升高（P<0.01），小鼠结肠黏膜AQP3表达显著减少，AQP9表达显著增加（P<0.01）；与莫沙必利组相比，枳术丸组小鼠肠道推进率升高（P<0.05），血清木糖含量显著升高（P<0.01），结肠黏膜AQP3表达显著减少（P<0.01），AQP9表达显著增加（P<0.01），粪便含水量差异无统计学意义（P>0.05）。结论 STC的发病与结肠黏膜AQP3上调、AQP9下调有关，枳术丸可以通过下调AQP3的表达、上调AQP9的表达，增加粪便的含水量而改善STC。 |
| 关键词: 慢传输型便秘|枳术丸|脾虚证|结肠黏膜|AQP3|AQP9 |
| DOI：10.3969/j.issn.1674－070X.２０23.06.006 |
| Received:October 18, 2022 |
| 基金项目:湖南省自然科学基金青年项目（2020JJ5425）；湖南省中医药科研计划重点项目（C2022033）；湖南省教育厅重点项目（21A0232）。 |
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| Effects of Zhizhu Pill on AQP3 and AQP9 in colonic mucosa of mice with slow transit constipation of spleen deficiency pattern |
| TANG Wenjuan,XIA Xuting,LIU Fulin |
| (Graduate School, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;College of Chinese Medicine, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To observe the effects of Zhizhu Pill on the expressions of AQP3 and AQP9 in the colon of mice with slow transit constipation (STC) of spleen deficiency pattern, and to explore the possible mechanism of Zhizhu Pill in improving STC symptoms. Methods The total of 50 SPF Kunming mice were randomly divided into normal group (n=15) and model-building group (n=35). The STC mouse model of spleen deficiency pattern was established by compound factor method [Fanxieye (Sennae Folium)+ water restriction + diet control]. After successful modeling, the mice of model-building group were randomly divided into model group (n=10), Zhizhu Pill group (n=10), and Mosapride group (n=10) according to their body weight. After 7 days of continuous drug administration, the fecal water content, intestinal propulsion rate, serum xylose content as well as histopathological characteristics of colonic mucosa were determined. Immunohistochemistry and Western blot were used to measure the expressions of AQP3 and AQP9 proteins. Results Compared with normal group, the fecal water content, the intestinal propulsion rate, and the serum xylose content of the mice in model group were significantly lower, the expression of AQP3 in mice colonic mucosa was significantly higher, and the expression of AQP9 was significantly lower (P<0.01). Compared with model group, the fecal water content, the intestinal propulsion rate, and the serum xylose content of Zhizhu Pill group were significantly higher (P<0.05, P<0.01, P<0.01), the expression of AQP3 in mice colonic mucosa was significantly lower, and the expression of AQP9 was significantly higher (P<0.01). Compared with mosapride group, the intestinal propulsion rate and the serum xylose content of Zhizhu Pill group were significantly higher (P<0.05, P<0.01), the expression of AQP3 in mice colonic mucosa was significantly lower（P<0.01）, and the expression of AQP9 was significantly higher (P<0.01). The difference of fecal water content between the two groups was of no statistical significance (P>0.05). Conclusion The pathogenesis of slow transit constipation is related to the up-regulation of AQP3 and the down-regulation of AQP9 in colonic mucosa; Zhizhu Pill can relieve slow transit constipation by down-regulating the expression of AQP3, up-regulating the expression of AQP9, and increasing the water content of stool. |
| Key words: slow transit constipation|Zhizhu Pill|spleen deficiency pattern|colonic mucosa|AQP3|AQP9 |
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