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周慧,肖长江,夏相宜,鄢磊,尹晓芳,莫凌,王孟可.四磨汤对心力衰竭大鼠肠道菌群及氧化三甲胺的影响[J].湖南中医药大学学报英文版,2023,43(5):790-798.[Click to copy
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| 四磨汤对心力衰竭大鼠肠道菌群及氧化三甲胺的影响 |
| 周慧,肖长江,夏相宜,鄢磊,尹晓芳,莫凌,王孟可 |
| (湖南中医药大学, 湖南 长沙 410208;湖南省中医药研究院附属医院, 湖南 长沙 410006) |
| 摘要: |
| 目的 观察肠道-氧化三甲胺(trimethylamine oxide, TMAO)-心力衰竭(heart failure, HF)轴上四磨汤对HF大鼠肠道菌群及TMAO的影响,初步探讨四磨汤从肠道菌群方面防治HF的可能机制。方法 选用40只雄性大鼠,运用随机数字表法分为对照组、四磨汤对照组、模型组、四磨汤模型组,每组10只。对照组、四磨汤对照组予生理盐水2.5 mg/kg腹腔注射;模型组、四磨汤模型组予异丙肾上腺素2.5 mg/kg腹腔注射造模,4组大鼠连续4周腹腔注射结束后,连续灌胃治疗2周。对照组、模型组予生理盐水7.56 mL/kg灌胃,每日1次;四磨汤对照组、四磨汤模型组予四磨汤7.56 mL/kg灌胃,每日1次。使用液相色谱-质谱法检测血浆TMAO,ELISA法检测血清氨基端前脑钠素(N-terminal pro-brain natriuretic peptide, NT-proBNP),16S rRNA检测粪便样本,生物信息学方法分析与比较样品间的菌群结构特征。结果 模型组、四磨汤模型组TMAO、NT-proBNP含量均明显高于对照组(P<0.05,P<0.01),四磨汤模型组TMAO、NT-proBNP含量均明显低于模型组(P<0.05,P<0.01)。与对照组相比,四磨汤对照组乳酸杆菌含量明显升高(P<0.05),肠球菌含量明显减少(P<0.05);模型组乳酸杆菌、普氏菌、双歧杆菌含量均明显减少(P<0.05),肠球菌、大肠埃希菌含量均明显升高(P<0.05);四磨汤模型组普氏菌含量明显减少(P<0.05),肠球菌、大肠埃希菌含量均明显升高(P<0.05)。与模型组相比,四磨汤模型组普氏菌含量明显升高(P<0.05),肠球菌、大肠埃希菌含量均明显降低(P<0.05)。结论 四磨汤可以降低HF大鼠TMAO、NT-proBNP含量,其机制可能与四磨汤改变HF大鼠肠道菌群、肠道-TMAO-HF轴相关。 |
| 关键词: 心力衰竭 四磨汤 肠道菌群 氧化三甲胺 氨基端前脑钠素 生物信息学 |
| DOI：10.3969/j.issn.1674-070X.2023.05.006 |
| Received:August 13, 2022 |
| 基金项目:国家中医药管理局全国名老中医药专家传承工作室建设项目（国中医药人教发〔2013〕47号）；湖南省中医药管理局重点课题（2021008）；湖南省“十四五”中医药学科带头人培养计划项目（湘中医〔2022〕4号）。 |
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| Effects of Simo Decoction on intestinal flora and TMAO in rats with heart failure |
| ZHOU Hui,XIAO Changjiang,XIA Xiangyi,YAN Lei,YIN Xiaofang,MO Ling,WANG Mengke |
| (Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;The Hospital of Hunan Academy of Chinese Medicine, Changsha, Hunan 410006, China) |
| Abstract: |
| Objective To observe the effects of Simo Decoction on intestinal flora and trimethylamine oxide (TMAO) in rats with heart failure (HF) on intestinal-TMAO-HF axis, and to preliminarily explore the possible mechanism of Simo Decoction in preventing and treating HF from the intestinal flora. Methods Forty male rats were selected and divided into control group, Simo Decoction control group, model group and Simo Decoction model group by random number table method, with 10 rats in each group. The control group and Simo Decoction control group received intraperitoneal injection of normal saline 2.5 mg/kg; model group and Simo Decoction model group were given isoprenaline 2.5 mg/kg to establish model by intraperitoneal injection. After 4 weeks of intraperitoneal injection, the rats in 4 groups were continuously treated with gastric gavage for 2 weeks. The control group and the model group were given normal saline 7.56 mL/kg by gastric gavage, once a day; the Simo Decoction control group and Simo Decoction model group were given Simo Decoction 7.56 mL/kg by gastric gavage, once a day. liquid chromatograph-mass spectrometer was used to determine rat plasma TMAO, ELISA was used to measure N-terminal pro-brain natriuretic peptide (NT-proBNP) in rat serum, 16S rRNA was used to examine rat stool samples, and bioinformatics method was used to analyze and compare flora structure characteristics among samples. Results The TMAO and NT-proBNP content in plasma in model group and Simo Decoction model group was significantly higher than that in control group (P<0.05, P<0.01), and the TMAO and NT-proBNP content in plasma in Simo Decoction model group was lower than that in model group (P<0.05, P<0.01). Compared with control group, the content of Lactobacillus dramatically increased in Simo Decoction control group (P<0.05), the content of Enterococcus decreased significantly (P<0.05); the content of Lactobacillus, Prevotella and Bifidobacterium dramatically decreased in model group (P<0.05), the content of Enterococcus and Escherichia coli increased significantly (P<0.05); the content of Prevotella dramatically decreased in Simo Decoction model group (P<0.05), the content of Enterococcus and Escherichia coli increased significantly (P<0.05). Compared with model group, the content of Prevotella dramatically increased in Simo Decoction model group (P<0.05), the content of Enterococcus and Escherichia coli decreased significantly (P<0.05). Conclusion Simo Decoction can reduce the content of TMAO and NT-proBNP in HF rats, and the mechanism may be related to the change of intestinal flora and intestinal-TMAO-HF axis in HF rats induced by Simo Decoction. |
| Key words: heart failure Simo Decoction intestinal flora trimethylamine oxide N-terminal pro-brain natriuretic peptide bioinformatics method |
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