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朱智显,张小琴,蔡虎志,别明珂,曾昭文,欧阳过,陈新宇.天牛止眩胶囊对自发性高血压大鼠心肌lncRNA MEG3/miR-21调控PTEN诱导左心室肥厚的影响[J].湖南中医药大学学报英文版,2023,43(3):437-442.[Click to copy
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| 天牛止眩胶囊对自发性高血压大鼠心肌lncRNA MEG3/miR-21调控PTEN诱导左心室肥厚的影响 |
| 朱智显,张小琴,蔡虎志,别明珂,曾昭文,欧阳过,陈新宇 |
| (湖南中医药大学第一附属医院, 湖南 长沙 410007) |
| 摘要: |
| 目的 分析天牛止眩胶囊对自发性高血压大鼠心肌组织lncRNA MEG3/miR-21/PTEN信号通路的影响及作用机制。方法 选取10只7周龄大鼠为正常对照组，随机将30只7周龄雄性自发性高血压大鼠分为模型对照组、天牛止眩组及依那普利组，每组10只，予以相应药物或蒸馏水灌胃8周。每周固定时间测量大鼠收缩压，直至第8周末处死大鼠，称量左心室质量（left ventricular mass, LVM），计算左心室质量指数（left ventricular mass index, LVMI），采用实时荧光定量PCR法检测心肌组织lncRNA MEG3、miR-21基因的表达水平，Western blot检测PTEN蛋白的表达水平。结果 治疗结束后，与正常对照组比较，模型对照组收缩压、LVM、LVMI和miR-21基因表达均升高（P＜0.05，P＜0.01），lncRNA MEG3基因、PTEN蛋白表达均降低（P＜0.01）；与模型对照组比较，天牛止眩组和依那普利组收缩压、LVM、LVMI和miR-21基因表达均降低（P<0.01），lncRNA MEG3基因、PTEN蛋白表达均升高（P<0.01）；与依那普利组比较，天牛止眩组lncRNA MEG3基因、PTEN蛋白表达均升高（P<0.01），miR-21基因表达降低（P<0.01）。结论 天牛止眩胶囊能上调大鼠心肌组织lncRNA MEG3表达，使miR-21表达下降，进而升高PTEN蛋白水平，这可能是该药降低血压及减轻靶器官损伤重要机制之一。 |
| 关键词: 高血压 左心室肥厚 天牛止眩胶囊 lncRNA MEG3 miR-21 PTEN |
| DOI：10.3969/j.issn.1674-070X.2023.03.011 |
| Received:July 18, 2022 |
| 基金项目:全国名老中医药专家陈新宇传承工作室建设项目（国中医药人教函〔2022〕75号）；湖南省创新性省份建设专项抗击新冠肺炎项目(2020SK3009) ；湖南省科学技术厅重点领域研发计划（2019SK2321）；湖南省科技人才托举工程项目（2020TJ-N01）；湖南省发改委创新引导专项（湘发改投资2019-412号）；心病“四时调阳”重点研究室建设专项（湘中医药函〔2020〕51号）；“四时调阳”治未病湖南省工程研究中心（湘发改高技〔2020〕1006号）；湖南中医药大学中医学一流学科开放基金（2022ZYX01，2022ZYX11，2021ZYX41）。 |
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| Effects of Tianniu Zhixuan Capsule on left ventricular hypertrophy induced by PTEN regulated by lncRNA MEG3/miR-21 in spontaneously hypertensive rats |
| ZHU Zhixian,ZHANG Xiaoqin,CAI Huzhi,BIE Mingke,ZENG Zhaowen,OUYANG Guo,CHEN Xinyu |
| (The First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China) |
| Abstract: |
| Objective To analyze the effects and mechanism of Tianniu Zhixuan Capsule (TNZXC) on the left ventricular hypertrophy induced by lncRNA MEG3/miR-21/PTEN in spontaneously hypertensive rats. Methods Ten rats aged 7 weeks were chosen as the normal control group, while 30 male spontaneously hypertensive rats were randomly divided into three groups (10 rats in each group): model control group, TNZXC group, and enalapril group, among which the rats were infused with corresponding drugs or distilled water for 8 weeks. The systolic blood pressure of the rats was measured at a fixed time every week. At the end of 8th week, the rats were sacrificed, and the left ventricular mass (LVM) was weighed to calculate the left ventricular mass index (LVMI). Then the expression of lncRNA MEG3 and miR-21 gene in myocardial tissue as well as PTEN protein expression were detected by real-time fluorescence quantitative PCR and Western blot respectively. Results After treatment, compared with the normal control group, the systolic blood pressure, LVM, LVMI and miR-21 gene expression in the model control group increased (P<0.05, P<0.01), while the expression of lncRNA MEG3 gene and PTEN protein was reduced (P<0.01). Compared with the model control group, the systolic blood pressure, LVM, LVMI and miR-21 gene expression in the TNZXC and enalapril groups were reduced (P<0.01), while the expression of lncRNA MEG3 gene and PTEN protein increased (P<0.01). Compared with the enalapril group, the expression of lncRNA MEG3 gene and PTEN protein in the TNZXC group increased (P<0.01), while the expression of miR-21 was reduced (P<0.01). Conclusion TNZXC can up-regulate the expression of lncRNA MEG3 in myocardial tissue of rats, reducing the expression of miR-21to increase the level of PTEN protein in spontaneously hypertensive rats, which may be one of the most significant mechanisms of the drug to lower blood pressure and alleviate target organ damage. |
| Key words: hypertension left ventricular hypertrophy Tianniu Zhixuan Capsule lncRNA MEG3 miR-21 PTEN |
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