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荆晓朔,汪顺伟,欧颖,张少辉,李静,李亮.益气活血自拟方对冠心病气虚血瘀证大鼠心功能的影响[J].湖南中医药大学学报英文版,2023,43(2):211-217.[Click to copy
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| 益气活血自拟方对冠心病气虚血瘀证大鼠心功能的影响 |
| 荆晓朔,汪顺伟,欧颖,张少辉,李静,李亮 |
| (湖南中医药大学中医诊断学湖南省重点实验室, 湖南 长沙 410208;湖南中医药大学中医心肺病证辨证与药膳食疗重点研究室, 湖南 长沙 410208) |
| 摘要: |
| 目的 探讨益气活血自拟方（YQHXF）对冠心病气虚血瘀证大鼠心功能的保护作用。方法 将24只雄性SD大鼠随机分为假手术组、模型组、YQHXF组（17.1 g/kg）和西药组（单硝酸异山梨酯3.6 mg/kg），每组6只。除假手术组外，其余各组均采用“结扎冠状动脉左前降支合并游泳力竭”的方法制备冠心病气虚血瘀证动物模型，假手术组只穿线不结扎。造模结束后，分别对YQHXF组及西药组予以灌胃干预28 d。观察大鼠一般状态、体质量、心电图、血液流变学指标变化，彩色多普勒超声检查心功能，HE、Masson染色观察心脏组织。结果 造模后7、14、28 d，与假手术组比较，模型组体质量显著降低（P<0.01）；与模型组比较，YQHXF组和西药组体质量显著升高(P<0.01)。造模后7、14 d，与西药组比较，YQHXF组体质量显著降低（P<0.05，P<0.01）。与假手术组比较，模型组一般状态变差，心电图ST段抬高，心肌纤维排列紊乱，心肌细胞出现肿大、坏死，且有炎症细胞浸润，心肌纤维化增加；左心室射血分数（left ventricular ejection fraction, LVEF）、左心室短轴缩短率（left ventricular fraction shortening, LVFS）均显著降低（P<0.01），左心室舒张末期容积（left ventricular end-diastolic volume, LVEDV）、左心室收缩末期容积（left ventricular end-systolic volume, LVESV）、左心室舒张末期内径（left ventricular end-diastolic internal dimension, LVEDID）、左心室收缩末期内径（left ventricular end-systolic internal dimension, LVESID）、红细胞聚集指数、卡松黏度、全血黏度（低、中、高切黏度）、胶原容积分数均显著升高（P<0.01）。与模型组比较，YQHXF组和西药组一般状态改善，心电图ST段降低，心肌纤维排列整齐，心肌细胞结构及形态改善，炎症细胞减少，心肌纤维化减少；LVEF、LVFS均显著升高（P<0.01），LVEDV、LVESV、LVEDID、LVESID、红细胞聚集指数、卡松黏度、全血黏度（低、中、高切黏度）、胶原容积分数均显著降低（P<0.01）。与西药组比较，YQHXF组心肌纤维减少；高切黏度、胶原容积分数显著降低（P<0.01）。结论 YQHXF可以明显改善大鼠一般状态，调节血液流变学指标，抑制心肌组织病理损伤，提高心功能，从而有效治疗冠心病气虚血瘀证。 |
| 关键词: 益气活血自拟方 冠心病 气虚血瘀证 病证结合 动物模型 |
| DOI：10.3969/j.issn.1674-070X.2023.02.006 |
| Received:July 20, 2022 |
| 基金项目:国家自然科学基金项目（81874429）；湖南省教育厅重点项目（19A380）；湖南省中医药科研计划项目重点项目（202004）。 |
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| Intervention effects of self-drafted Yiqi Huoxue Formula on heart function of rats with coronary heart disease of qi deficiency induced blood stasis pattern |
| JING Xiaoshuo,WANG Shunwei,OU Ying,ZHANG Shaohui,LI Jing,LI Liang |
| (Provincial Key Laboratory of TCM Diagnostics, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Key Laboratory of TCM Heart and Lung Pattern Differentiation & Medicated Diet and Dietotherapy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To investigate the protective effects of self-drafted Yiqi Huoxue Formula (YQHXF) on the heart function in rats with coronary heart disease of qi deficiency induced blood stasis pattern. Methods A total of 24 male SD rats were randomly divided into sham operation group, model group, YQHXF (17.1 g/kg) group and western medicine group (isosorbide mononitrate 3.6 mg/kg), with 6 rats in each group. Except for sham operation, animal models of coronary heart disease of qi deficiency induced blood stasis pattern were prepared by "ligation of left anterior descending coronary artery and swimming exhaustion" in the other groups. Only thread without ligation for the sham operation group was conducted. After modeling, YQHXF group and western medicine group were given gavage intervention for 28 d. The general state, body weight, electrocardiogram and hemorheology indexes of the rats were observed. The cardiac function was tested by color Doppler ultrasound, and the heart tissues were observed by HE and Masson staining. Results Compared with sham operation group, the body weight of model group decreased significantly 7, 14 and 28 d after modeling (P<0.01). Compared with model group, body weight of YQHXF group and western medicine group significantly increased 7, 14 and 28 d after modeling (P<0.01). Compared with sham operation group, the general state of the model group became worse, the ST segment of the electrocardiogram was significantly elevated, the arrangement of myocardial fibers was obviously disordered, the myocardial cells were swollen, necrotic and infiltrated by inflammatory cells, and the myocardial fibrosis increased; left ventricular ejection fraction (LVEF) and left ventricular fraction shortening (LVFS) of model group were lower (P<0.01), while left ventricular end-diastolic volume (LVEDV), left ventricular end-systolic volume (LVESV), left ventricular end-diastolic internal dimension (LVEDID), left ventricular end-systolic internal dimension (LVESID), erythrocyte aggregation index, Casson viscosity, whole blood viscosity (low, medium and high shear viscosity) and collagen volume fraction were higher (P<0.01). Compared with model group, the general state of the YQHXF group and the western medicine group was improved, the ST segment of the electrocardiogram was reduced, the myocardial fibers were arranged neatly, the structure and morphology of the myocardial cells were improved, the inflammatory cells and the myocardial fibrosis were less. LVEF and LVFS of YQHXF group and western medicine group were higher (P<0.01), while LVEDV, LVESV, LVEDID, LVESID, erythrocyte aggregation index, Carson viscosity, whole blood viscosity (low, medium and high shear viscosity) and collagen volume fraction were lower (P<0.01). Compared with western medicine group, the myocardial fibers in the YQHXF group were reduced; high shear viscosity and collagen volume fraction decreased significantly (P<0.01). Conclusion Self-drafted Yiqi Huoxue Formula can significantly improve the general state of rats, regulate blood rheological indicators, inhibit myocardial tissue pathological damage, and improve cardiac function, thereby effectively treating coronary heart disease of qi deficiency induced blood stasis pattern. |
| Key words: self-drafted Yiqi Huoxue Formula coronary heart disease qi deficiency induced blood stasis pattern differentiation of disease and pattern animal model |
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