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李宗超,孙康,汪旻峻,迟呈林,王炜,刘荣霞.基于入血成分和网络药理学探讨降香治疗急性心肌梗死的潜在机制[J].湖南中医药大学学报英文版,2023,43(1):80-87.[Click to copy
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| 基于入血成分和网络药理学探讨降香治疗急性心肌梗死的潜在机制 |
| 李宗超,孙康,汪旻峻,迟呈林,王炜,刘荣霞 |
| (烟台大学药学院, 新型制剂与生物技术药物研究山东省高校协同创新中心、分子药理和药物评价教育部重点实验室, 山东 烟台 264005;湖南中医药大学药学院, 湖南 长沙 410208) |
| 摘要: |
| 目的 基于降香入血成分，借助网络药理学探讨降香治疗急性心肌梗死（acute myocardial infarction,AMI）的潜在活成分和机制。方法 利用UHPLC-Q-Orbitrap HRMS技术鉴定降香入血成分，并检索在线数据库查找成分和AMI靶点。借助STRING 11.5数据库与Cytoscape 3.7.0软件对蛋白质相互作用（protein-protein interaction,PPI）网络进行构建与分析，进一步通过DAVID数据库进行GO功能富集和KEGG通路注释分析，并对关键靶点进行分子对接验证。结果 鉴定降香入血成分24个，并筛选出染料木素、柚皮素等潜在活性成分，以及肉瘤基因（sarcoma gene,SRC）、表皮生长因子受体（epithelial growth factor receptor,EGFR）等关键靶点。富集结果显示，降香治疗AMI参与PI3K/AKT、FOXO等信号通路，以及受体结合、激酶活性等功能的调节。对接结果显示，关键靶点与成分有较好的结合性。结论 降香可能通过抗炎、抗氧化和促进血管新生治疗AMI。 |
| 关键词: 降香 急性心肌梗死 UHPLC-Q-Orbitrap HRMS 入血成分 网络药理学 分子对接 |
| DOI：10.3969/j.issn.1674-070X.2023.01.012 |
| Received:September 16, 2022 |
| 基金项目:国家自然科学基金项目（81973513）。 |
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| Potential mechanism of Jiangxiang (Dalbergia Odorifera) in treating acute myocardial infarction based on the components absorbed into the blood and network pharmacology |
| LI Zongchao,SUN Kang,WANG Minjun,CHI Chenglin,WANG Wei,LIU Rongxia |
| (Key Laboratory of Molecular Pharmacology and Drug Evaluation, Ministry of Education, Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, School of Pharmacy, Yantai University, Yantai, Shandong 264005, China;School of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To explore the potential mechanism of Jiangxiang (Dalbergia Odorifera) in the treatment of acute myocardial infarction (AMI) through network pharmacology based on the components absorbed into the blood. Methods UHPLC-Q-Orbitrap HRMS technology was used to identify the blood components of Jiangxiang (Dalbergia Odorifera), and online databases were searched to find the targets of components and AMI. The protein-protein interaction (PPI) network was constructed and analyzed by STRING 11.5 database and Cytoscape 3.7.0 software. GO function enrichment analysis and KEGG pathway annotation analysis were performed by DAVID database, and the hub targets were verified by molecular docking. Results Twenty-four components of Jiangxiang (Dalbergia Odorifera) that were absorbed into the blood were identified and potential active components such as genistein and naringenin, and hub targets such as sarcoma gene (Src) and epithelial growth factor receptor (EGFR) were screened. The enrichment results showed that the treatment of AMI with Jiangxiang (Dalbergia Odorifera) may involve the regulation of PI3K/AKT and FOXO signaling pathways, and the functions of apoptosis process and kinase activity. Molecular docking indicated that the hub targets had superior binding with the components. Conclusion AMI may be treated by Jiangxiang (Dalbergia Odorifera), that can takes anti-inflammatory and antioxidant effects, and promote angiogenesis. |
| Key words: Jiangxiang (Dalbergia Odorifera) acute myocardial infarction UHPLC-Q-Orbitrap HRMS components absorbed into the blood network pharmacology molecular docking |
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