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谭素芳,程晓,徐翠萍,钟昕,周琦,刘朝圣.防脱育发精华液对雄激素性脱毛大鼠局部皮肤睾酮、双氢睾酮、肝细胞生长因子水平的影响[J].湖南中医药大学学报英文版,2023,43(1):9-13.[Click to copy
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| 防脱育发精华液对雄激素性脱毛大鼠局部皮肤睾酮、双氢睾酮、肝细胞生长因子水平的影响 |
| 谭素芳,程晓,徐翠萍,钟昕,周琦,刘朝圣 |
| (湖南中医药高等专科学校, 湖南 株洲 412012;湖南中医药大学第一附属医院皮肤科, 湖南 长沙 410007;湖南中医药高等专科学校附属第一医院, 湖南 株洲 412000) |
| 摘要: |
| 目的 探讨防脱育发精华液对雄激素性脱毛大鼠局部皮肤组织睾酮（testosterone,T）、双氢睾酮（dihydrotestosterone,DHT）、肝细胞生长因子（hepatocyte growth factor,HGF）水平的影响。方法 将45只大鼠随机分为空白组、对照组、模型组、米诺地尔酊组、防脱育发液精华液组，每组9只。模型组、米诺地尔酊组、防脱育发精华液组连续6周皮下注射丙酸睾酮注射液(5mg/kg)建立雄激素性脱毛大鼠模型，对照组皮下注射生理盐水，造模同时给药，实验过程中定期观察大鼠一般状态，运用免疫组化、Western blot、ELISA等技术手段，检测大鼠局部皮肤T、DHT、HGF表达的变化。结果 与空白组比较，模型组T、DHT含量均明显升高（P<0.05，P<0.01），HGF蛋白表达水平明显降低（P<0.05）。与模型组比较，米诺地尔酊组和防脱育发精华液组T、DHT含量均明显降低（P<0.05，P<0.01），防脱育发精华液组HGF蛋白表达水平明显升高（P<0.05）。与米诺地尔酊组比较，防脱育发精华液组DHT含量明显降低（P<0.05）。结论 防脱育发精华液可减轻雄激素性脱毛大鼠脱毛程度，通过降低局部皮肤组织T、DHT含量，促进HGF表达是其主要作用机制之一。 |
| 关键词: 雄激素性脱发 防脱育发精华液 睾酮 双氢睾酮 肝细胞生长因子 |
| DOI：10.3969/j.issn.1674-070X.2023.01.002 |
| Received:April 16, 2022 |
| 基金项目:湖南中医药大学第一附属医院国医大师熊继柏传承工作室基金项目（XJB202208）；湖南中医药大学中西医结合一流学科开放基金项目（2020ZXYJH06）。 |
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| Effects of Fangtuo Yufa Essence on the T, DHT and HGF levels in the local skin of androgenic alopecia rats |
| TAN Sufang,CHENG Xiao,XU Cuiping,ZHONG Xin,ZHOU Qi,LIU Chaosheng |
| (Hunan Chinese Medical College, Zhuzhou, Hunan 412012, China;The First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;The First Hospital of Hunan College of Chinese Medicine, Zhuzhou, Hunan 412000, China) |
| Abstract: |
| Objective To investigate the effects of Fangtuo Yufa Essence on the levels of testosterone (T), di-hydrotestosterone (DHT), hepatocyte growth factor (HGF) in the local skin tissue of SD androgenic alopecia rats. Methods A total of 45 rats were randomly divided into blank group, control group, model group, minoxidil group, and Fangtuo Yufa Essence group, with 9 in each group. For six weeks, the rat models of androgenic alopecia were established by subcutaneous testosterone propionate injection (5 mg/kg) for 6 consecutive weeks in model group, minoxidil group, and Fangtuo Yufa Essence group. While the control group was subcutaneously injected with an equal volume of normal saline. Meanwhile, the drug was evenly applied during modeling. During the experiment, the general conditions, the expression levels of T, DHT and HGF of each group were detected utilizing immunohistochemistry, Western blot, ELISA, and others. Results The content of T and DHT was significantly higher (P<0.05, P<0.01) and the HGF protein expression levels were significantly lower (P<0.05) in model group versus blank group. Compared with the model group, the minoxidil group and Fangtuo Yufa Essence group showed lower T and DHT content (P<0.05, P<0.01), while Fangtuo Yufa Essence group showed higher HGF protein expression levels (P<0.05). The DHT content in Fangtuo Yufa Essence group decreased apparently in comparison to the minoxidil group (P<0.05). Conclusion Fangtuo Yufa Essence can alleviate the hair removal in androgenic alopecia rats. One of the main mechanisms may be decreasing T and DHT content and increasing HGF expression in local skin tissues. |
| Key words: androgenic alopecia Fangtuo Yufa Essence testosterone di-hydrotestosterone hepatocyte growth factor |
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