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匡小霞,蒋全睿,吴琼,赖畇绮,冯祥,朱晓晴,李江山,李武.按法干预对大鼠激痛点骨骼肌细胞骨架α-tubulin和MAP-4的影响[J].湖南中医药大学学报英文版,2022,42(11):1891-1896.[Click to copy
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| 按法干预对大鼠激痛点骨骼肌细胞骨架α-tubulin和MAP-4的影响 |
| 匡小霞,蒋全睿,吴琼,赖畇绮,冯祥,朱晓晴,李江山,李武 |
| (湖南中医药大学针灸推拿与康复学院, 湖南 长沙 410208) |
| 摘要: |
| 目的 通过观察按法干预对激痛点骨骼肌细胞骨架α微管蛋白（α-tubulin）和微管相关蛋白4（microtubule-associated protein 4, MAP-4）的影响，探讨按法的舒筋解结作用。方法 40只SPF级雄性大鼠随机分为空白组10只和激痛点造模大鼠30只，采用钝性打击结合离心运动的方法建立大鼠激痛点模型，模型评价后将符合标准的20只大鼠随机分为模型组和按法组，每组10只。空白组和模型组不予按法干预，仅正常观察，按法组以自制按法刺激器干预14 d。干预结束后在激痛点局部取材，用Western blot和免疫荧光双染法检测α-tubulin和MAP-4。结果 α-tubulin和MAP-4在肌细胞和细胞外基质均有一定的共表达，空白组α-tubulin和MAP-4主要分布在肌细胞膜与细胞质，分布连续性较好，模型组α-tubulin分布连续性和完整性欠佳，在肌细胞内表达减少，在细胞外基质表达增加，MAP-4在肌细胞和细胞外基质表达均增加，两者在按法组有一定改善。与空白组相比，模型组α-tubulin表达下降、MAP-4表达升高（P＜0.05）；与模型组相比，按法组α-tubulin表达升高、MAP-4表达下降（P＜0.05）。结论 按法的舒筋解结作用可能与抑制微管蛋白解聚并促进其合成有关。 |
| 关键词: 按法 激痛点 骨骼肌 舒筋解结 细胞骨架 微管 α微管蛋白 微管相关蛋白4 |
| DOI：10.3969/j.issn.1674-070X.2022.11.019 |
| Received:June 26, 2022 |
| 基金项目:国家自然科学基金面上项目（8197395，82174526，82274676）；湖南省自然科学基金青年项目（2021JJ40482）。 |
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| Effects of pressing intervention on α-tubulin and MAP-4 in skeletal muscle cytoskeleton at the myofascial trigger points in rats |
| KUANG Xiaoxia,JIANG Quanrui,WU Qiong,LAI Yunqi,FENG Xiang,ZHU Xiaoqing,LI Jiangshan,LI Wu |
| (College of Acupuncture & Tuina and Rehabilitation, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To investigate the relaxing tendon and resolving knot effects of pressing intervention by observing the effects of pressing on α-tubulin and microtubule-associated protein 4 (MAP-4) of the skeletal muscle cytoskeleton at the myofascial trigger points (MTrPs) in rats. Methods Forty SPF male rats were randomly divided into the blank group of 10 rats and the MTrPs model group of 30 rats. The MTrPs modeling was established by blunt strikes combined with centrifugal movement. After model evaluation, 20 rats meeting the criteria were randomly divided into model group and pressing group, with 10 rats in each group. The blank group and the model group were not pressed and only observed normally, and the pressing group was pressed by a homemade pressing stimulator for 14 d. After intervention, local samples were taken at the MTrPs and α-tubulin and MAP-4 were detected by Western blot and immunofluorescence double-staining method. Results α-tubulin and MAP-4 were co-expressed in both myocytes and extracellular matrix. In the blank group, α-tubulin and MAP-4 were mainly distributed in the myocyte membrane and cytoplasm, and the distribution continuity was good. In the model group, the distribution continuity and integrity of α-tubulin were poor, with decreased expression in myocytes and increased expression in the extracellular matrix. MAP-4 expression in the model group increased in both myocytes and extracellular matrix. Both showed some improvement in the press group. Compared with the blank group, α-tubulin expression decreased and MAP-4 expression increased in the model group (P＜0.05); compared with the model group, α-tubulin expression increased and MAP-4 expression decreased in the pressing group (P＜0.05). Conclusion The relaxing tendon and resolving knot effects of pressing intervention may be related to inhibiting microtubule protein depolymerization and promoting its synthesis. |
| Key words: pressing myofascial trigger points skeletal muscle relaxing tendon and resolving knot cytoskeleton microtubules α-tubulin microtubule-associated protein 4 |
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