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廖颖妍,汪宇馨,秦思雨,夏伯候.基于UPLC-Q-TOF/MS技术分析妇科千金胶囊化学成分[J].湖南中医药大学学报英文版,2022,42(11):1876-1883.[Click to copy
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| 基于UPLC-Q-TOF/MS技术分析妇科千金胶囊化学成分 |
| 廖颖妍,汪宇馨,秦思雨,夏伯候 |
| (湖南中医药大学药学院, 湖南 长沙 410208;湖南中医药大学湘产大宗药材品质评价湖南省重点实验室, 湖南 长沙 410208) |
| 摘要: |
| 目的 利用超高效液相色谱串联四级杆飞行时间质谱联用技术（ultra-high liquid chromatography tandem quadrupole-time-of-flight mass spectrometry, UPLC-Q-TOF/MS）系统分析妇科千金胶囊化学成分，为其治疗子宫肌瘤和盆腔炎药效的物质基础和作用机制提供科学依据。方法 采用KROMASIL 100-5-C18色谱柱（4.6 mm×250 mm，5 μm），以0.1%甲酸水和乙腈为流动相进行梯度洗脱，流速0.3 mL/min，柱温：30 ℃；在质谱电喷雾（ESI）正、负离子模式下检测。使用在线化学成分数据库建立包括化合物名称、分子式和精确相对分子质量的妇科千金胶囊各药材化学成分数据库，将UPLC-Q-TOF/MS扫描的化合物信息与数据库比对，并结合对照品的质谱数据、相对保留时间及文献参考等来对妇科千金胶囊中的化学成分进行结构鉴定。结果 从妇科千金胶囊中初步鉴定出35个化合物，其中生物碱类10个、萜类7个、苯酞类化合物3个、黄酮类化合物8个、有机酸类化合物6个、糖苷类1个。结论 UPLC-Q-TOF/MS技术能快速全面地对妇科千金胶囊进行化学成分分析，为其药效物质基础的进一步研究奠定基础和提供科学依据。 |
| 关键词: 妇科千金胶囊 化学成分 超高效液相色谱串联四级杆飞行时间质谱 生物碱 |
| DOI：10.3969/j.issn.1674-070X.2022.11.017 |
| Received:May 20, 2022 |
| 基金项目:长沙市科技计划项目（kq2004055）。 |
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| Chemical composition analysis of Fuke Qianjin Capsule based on UPLC-Q-TOF/MS technology |
| LIAO Yingyan,WANG Yuxin,QIN Siyu,XIA Bohou |
| (College of Pharmacy, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China;Key Laboratory of Hunan Province for Quality Evaluation of Bulk Medicinal Materials Produced in Hunan Province, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To analyze the composition of Fuke Qianjin Capsule by ultra performance liquid chromatography tandem quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF/MS) system and to provide scientific basis for the material basis and mechanism of treating uterine fibroids and pelvic inflammatory disease. Methods The samples were prepared on a KROMASIL 100-5-C18 column（4.6 mm×250 mm, 5 μm） with the mobile phases of acetonitrile (A) and 0.1% formic acid aqueous solution (B) in gradient elution with the flow rate of 0.300 mL/min, the column temperature of 30 ℃, and the injection volume of 10 μL. The samples were detected in positive and negative ion modes by electrospray ionization (ESI). Online chemical composition database was used to establish chemical composition database of Fuke Qianjin Capsule, including compound name, molecular formula and accurate relative molecular weight. The compound information scanned by UPLC-Q-TOF/MS was compared with the database, and the structure of the chemical composition in Fuke Qianjin Capsule were identified by combining the mass spectrometry data, relative retention time and literature references. Results A total of 35 compounds were identified from Fuke Qianjin Capsule, including 10 alkaloids, 7 terpenoids, 3 phthalides, 8 flavonoids, 6 organic acids and 1 glycoside. Conclusion UPLC-Q-TOF/MS technology could quickly and comprehensively analyze the chemical compositions of Fuke Qianjin Capsule, laying the foundation and providing scientific basis for further study of its pharmacodynamic substance basis. |
| Key words: Fuke Qianjin Capsule chemical composition UPLC-Q-TOF-MS alkaloids |
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