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郭忠聪,杨宇婷,王智贤,龚纯.抗癌防移片对结直肠癌肝转移BALB/c小鼠P65、STAT3、TNF-α因子的影响[J].湖南中医药大学学报英文版,2022,42(11):1823-1829.[Click to copy
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| 抗癌防移片对结直肠癌肝转移BALB/c小鼠P65、STAT3、TNF-α因子的影响 |
| 郭忠聪,杨宇婷,王智贤,龚纯 |
| (湖南中医药大学第一附属医院肿瘤科, 湖南 长沙 410007;湖南中医药大学研究生院, 湖南 长沙 410208) |
| 摘要: |
| 目的 通过研究抗癌防移片对结直肠癌肝转移小鼠模型肝脏组织中癌基因蛋白质p65（oncogene protein p65, P65）、信号传导转录激活因子3（signal transduction and activator of transcription 3, STAT3）蛋白表达，以及血清中肿瘤坏死因子-α（tumor necrosis factor-α, TNF-α）浓度检测，探讨抗癌防移片预防结直肠癌肝转移的作用机制。方法 采用脾脏注入CT26细胞的方法制备结直肠癌小鼠模型，按照随机分配的原则分为6组：模型组、阳性对照组、中药低剂量组、中药中剂量组、中药高剂量组、中西药组，每组5只。模型组予以等体积生理盐水灌胃，1次／d；阳性对照组予以奥沙利铂甘露醇注射液(L-OHP)、氟尿嘧啶注射液（5-Fu）、亚叶酸钙注射液(CF)，腹腔注射，1次/周；中药低剂量组、中药中剂量组、中药高剂量组裸鼠以抗癌防移片中药混悬液灌胃，1次/d；中西药组采用阳性对照组和中药高剂量组两种方式结合给药。分组干预3周后，采集肝脏组织和血清，采用HE染色法观察各组小鼠肝组织病理切片分析细胞结构，采用Western bolt检测P65及STAT3蛋白表达量，ELISA法检测血清中TNF-α蛋白浓度。结果 给药后3周，与模型组比较，中药高剂量组、中西药组、阳性对照组小鼠的体质量均明显增加（P＜0.05）；小鼠肝组织瘤体质量均显著降低（P＜0.05）。与模型组比较，阳性对照组、抗癌防移片高剂量组、中西药组小鼠组织细胞排列整齐，结构清晰，仅可见少许异型细胞。与模型组比较，抗癌防移片高剂量组P65、STAT3蛋白表达水平及TNF-α浓度含量降低（P＜0.05）。结论 抗癌防移片可能通过抑制P65、STAT3、TNF-α蛋白表达，改善组织炎症环境，从而发挥抑制结直肠癌肝转移的作用。 |
| 关键词: 结直肠癌 肝转移 抗癌防移片 炎症环境 癌基因蛋白质p65 信号传导转录激活因子3 肿瘤坏死因子 |
| DOI：10.3969/j.issn.1674-070X.2022.11.009 |
| Received:September 01, 2022 |
| 基金项目:湖南省卫生健康委一般项目（20200588）。 |
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| Effects of Anti-cancer and Anti-migration Tablets on the expression of P65, STAT3 and TNF-α factors in mice with liver metastasis of colorectal cancer |
| GUO Zhongcong,YANG Yuting,WANG Zhixian,GONG Chun |
| (Department of Oncology, The First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China;Graduate School, Hunan University of Chinese Medicine, Changsha, Hunan 410208, China) |
| Abstract: |
| Objective To discuss the mechanism of Anti-cancer and Anti-migration Tablets in preventing liver metastasis of colorectal cancer by investigating the expression of oncogene protein p65 (P65), signal transduction and activator of transcription 3 (STAT3) protein in liver tissues of mice model of liver metastasis of colorectal cancer, and the concentration of tumor necrosis factor (TNF-α) in serum. Methods The mice model of colorectal cancer was prepared by injecting CT26 cells into the spleen. According to the principle of random distribution, the mice were divided into 6 groups: model group, positive control group, low-dose group, medium-dose group, high-dose group, and Chinese and western medicine group. There were 5 mice in each group. The model group was given equal volume of normal saline by gavage once a day; The positive control group was given oxaliplatin mannitol injection (L-OHP), fluorouracil injection (5-Fu), calcium folinate injection (CF), intraperitoneal injection once a week; The low dose group, middle dose group and high dose group of Chinese medicine were administered orally with anti-cancer anti migration tablet suspension of Chinese medicine once a day; The positive control group and the high dose group of traditional Chinese medicine were used in the Chinese and western medicine group.After 3 weeks of intervention, liver tissues and serum were collected, and cell structures were analyzed by observing the pathological sections of liver tissues using HE staining. Western bolt was used to detect the expression of P65 and STAT3 proteins, and ELISA was applied to detect TNF in serum-α protein concentration. Results Three weeks after administration, compared with the model group, the body mass of mice in the high-dose Anti-cancer and Anti-migration Tablets group, the Chinese and western medicine group, and the positive control group increased significantly (P＜0.05); the tumor mass of mice liver tissues was significantly reduced (P＜0.05). Compared with the model group, the positive control group, the high-dose Anti-cancer and Anti-migration Tablets group, and the Chinese and western medicine group showed orderly arranged tissue cells with clear structure, with only a few heterotypic cells. Compared with the model group, the expression level of P65, STAT3 proteins and the concentration of TNF-α in the high-dose Anti-cancer and Anti-migration Tablets group decreased (P＜0.05). Conclusion Anti-cancer and Anti-migration Tablets may inhibit P65, STAT3 and TNF-α protein expression, and improve the tissue inflammatory environment, so as to take effects in inhibiting liver metastasis of colorectal cancer. |
| Key words: colorectal cancer liver metastases Anti-cancer and Anti-migration Tablets inflammatory environment oncogene protein p65 signal transduction and activator of transcription 3 tumor necrosis factor |
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