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张娟娟,张曦,杨逸璇,康慰,谢小丽,朱明芳.加味三仁汤联合石榴皮多酚乳膏对湿热痰瘀型金黄地鼠痤疮模型脂质组学的影响[J].湖南中医药大学学报英文版,2022,42(10):1641-1649.[Click to copy
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| 加味三仁汤联合石榴皮多酚乳膏对湿热痰瘀型金黄地鼠痤疮模型脂质组学的影响 |
| 张娟娟,张曦,杨逸璇,康慰,谢小丽,朱明芳 |
| (湖南中医药大学第二附属医院, 湖南 长沙 410005;浏阳市中医医院, 湖南 浏阳 410300) |
| 摘要: |
| 目的 探讨加味三仁汤联合石榴皮多酚乳膏对湿热痰瘀型痤疮动物模型金黄地鼠皮脂腺斑的作用机制。方法 将金黄地鼠随机分为模型组、中药外治组、中药内服组、中药内外合治组，每组8只。采用复合因素（外部湿热环境+肥甘饮食+限制动物活动空间）构建湿热痰瘀型金黄地鼠痤疮模型。除模型组外，其余各组分别给予石榴皮多酚乳膏外用于两侧皮脂腺斑处（每侧1 mL/次）、加味三仁汤灌胃（680 mg·kg-1）、加味三仁汤灌胃联合石榴皮多酚乳膏外用（方法、给药量与外治组、内服组相同），2次/d，连续给药14 d。通过观察金黄地鼠一般状况、皮脂腺斑总面积及组织病理变化评价加味三仁汤联合石榴皮多酚乳膏对金黄地鼠痤疮模型的干预作用；采用液相色谱-串联质谱联用技术，结合多元统计分析方法，对金黄地鼠皮脂腺斑进行非靶向脂质组学分析，探讨二者内外合治干预湿热痰瘀型金黄地鼠痤疮模型的作用机制。结果 加味三仁汤联合石榴皮多酚乳膏可以改善金黄地鼠痤疮模型一般状况、抗皮脂腺斑增生和组织病理损伤。在两组对比所得的共同差异代谢物中，共鉴定出13个与金黄地鼠皮脂腺斑脂质代谢相关的潜在生物学标志物。代谢通路分析显示差异脂质分子与亚油酸代谢、甘油酯代谢、花生四烯酸代谢、α-亚麻酸代谢相关。结论 加味三仁汤联合石榴皮多酚乳膏内外合治与模型组之间存在明显的脂质含量差异，这些差异表达的脂质分子可能是二者内外合治对湿热痰瘀型痤疮模型的干预靶点，二者内外合治对湿热痰瘀型痤疮的治疗作用机制可能与调节脂肪酸类代谢通路、甘油酯类代谢通路有关。 |
| 关键词: 痤疮 加味三仁汤 石榴皮多酚乳膏 湿热痰瘀 金黄地鼠 液相色谱-串联质谱联用技术 |
| DOI：10.3969/j.issn.1674-070X.2022.10.009 |
| Received:April 19, 2022 |
| 基金项目:国家自然科学基金项目（82174375）；湖南省中医药科研计划项目（2021093）；湖南省临床医疗技术创新引导项目（2020SK51501）；湖南省卫生健康委科研项目（202202124772）；湖南省中医药科研计划项目（C2022026）；湖南省高层次卫生人才“225”工程培养项目。 |
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| Effects of combining Modified Sanren Decoction and Pomegranate Peel Polyphenol Cream on lipidomics of golden hamsters acne model of damp-heat and phlegm-stasis type |
| ZHANG Juanjuan,ZHANG Xi,YANG Yixuan,KANG Wei,XIE Xiaoli,ZHU Mingfang |
| (The Second Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410005, China;Liuyang Hospital of Chinese Medicine, Liuyang, Hunan 410300, China) |
| Abstract: |
| Objective To investigate the mechanism of Modified Sanren Decoction combined with Pomegranate Peel Polyphenol Cream on the sebaceous spots of golden hamsters acne model of damp-heat and phlegm-stasis type. Methods Golden hamsters were randomly divided into model group, external treatment group with Chinese medicine, internal administration group with Chinese medicine, internal and external treatment group with Chinese medicine, with 8 hamsters in each group. A model of golden hamsters acne of damp-heat and phlegm-stasis type was constructed by compound factors (damp-heat external environment + fatty and sweet diet + restricted animal activity space). External treatment group with Chinese medicine was given Pomegranate Peel Polyphenols Cream for both sides of the sebaceous gland spots (1 mL per side each time); internal administration group with Chinese medicine recieved Modified Sanren Decoction by intragastric administration (680 mg/kg); internal and external treatment group with Chinese medicine was given Modified Sanren Decoction by intragastric administration and Pomegranate Peel Polyphenols Cream for both sides of the sebaceous gland spots (the method and dosage were the same as those of external treatment group with Chinese medicine and internal administration group with Chinese medicine). All interventions were twice a day for 14 d. The intervention effects of combining internal and external treatment on the acne model of golden hamsters were evaluated by observing the general condition, the total area of sebaceous gland spots and histopathological changes of golden hamsters; the non-targeted lipidomic analysis of sebaceous gland spots was carried out using liquid chromatography-tandem mass spectrometry (LC-MS/MS) technology combined with multivariate statistical analysis methods. The mechanism of the combined internal and external treatment with Chinese medicine in treating acne model of damp-heat and phlegm-stasis type was discussed. Results Modified Sanren Decoction combined with Pomegranate Peel Polyphenol Cream may improve general conditions of the golden hamsters model, inhibit hyperplasia of the sebaceous gland spots and histopathological damage. In the common differential metabolites obtained from the comparison of the two sets, we identified a total of 13 potential biological markers associated with lipid metabolism in the sebaceous gland spots of golden hamsters. Metabolic pathway analysis showed that differential lipid molecules were associated with the metabolisms of linoleic acid, glycerolipid, arachidonic acid, and α-linolenic acid. Conclusion There were significant differences in lipid content between internal and external treatment group with Chinese medicine and model group. The lipid molecules with differential expressions may be the intervention targets of the combining internal and external treatments for the acne models of damp-heat and phlegm-stasis type; the mechanism of the combining internal and external treatment for acne of damp-heat and phlegm-stasis type may be related to the regulation of fatty acid metabolism pathway and glyceride metabolic pathway. |
| Key words: acne Modified Sanren Decoction Pomegranate Peel Polyphenol Cream damp-heat and phlegm-stasis golden hamsters liquid chromatography-tandem mass spectrometry |
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