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王强,王玥.电针内关、百会通过NLRP3/ASC/Caspase-1通路干预局灶性脑缺血再灌注损伤大鼠的作用研究[J].湖南中医药大学学报英文版,2022,42(8):1335-1340.[Click to copy
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| 电针内关、百会通过NLRP3/ASC/Caspase-1通路干预局灶性脑缺血再灌注损伤大鼠的作用研究 |
| 王强,王玥 |
| (湖南省脑科医院, 湖南 长沙 410007;湖南中医药大学附属第一医院, 湖南 长沙 410007) |
| 摘要: |
| 目的 通过NOD样受体热蛋白结构域相关蛋白3（NOD-like receptor thermal protein domain associated protein 3,NLRP3）/凋亡相关斑点样蛋白（apoptosis-associated speck-like protein containing CARD,ASC）/半胱天冬酶-1（Caspase-1）通路探讨电针内关、百会对局灶性脑缺血再灌注损伤大鼠的干预作用及机制。方法 Sprague-Dawley大鼠30只，随机分为对照组、假手术组、模型组、尼莫地平组、电针组，每组6只。模型组、尼莫地平组、电针组予以大脑中动脉栓塞（middle cerebral artery occlusion,MCAO）造模，成功后尼莫地平组予10.8 mg/kg尼莫地平片灌胃，电针组予以针刺内关、百会，对照组、假手术组均给予0.9%生理盐水灌胃,干预14 d。采用Longa评分进行神经功能评定，采用ELISA法检测血清白介素-1β（interleukin-1 beta,IL-1β）含量，免疫荧光测定NLRP3、ASC、Caspase-1阳性表达。结果 造模后，模型组、尼莫地平组、电针组Longa评分明显升高，与对照组、假手术组相比，差异有统计学意义（P<0.05）；干预后，尼莫地平组、电针组Longa评分低于模型组（P<0.05），而尼莫地平组、电针组之间的Longa评分差异无统计学意义（P>0.05）。干预后，模型组大鼠IL-1β含量高于对照组、假手术组（P<0.01）；尼莫地平组、电针组大鼠IL-1β含量低于模型组（P<0.05）。干预后，模型组ASC、Caspase-1及NLRP3的表达明显高于对照组、假手术组（P<0.01）；与模型组比较，尼莫地平组、电针组ASC、Caspase-1及NLRP3的表达明显降低（P<0.01）。结论 电针内关、百会可改善局灶性脑缺血再灌注损伤大鼠运动功能，可能与调控NLRP3/ASC/Caspase-1信号通路、降低IL-1β表达有关。 |
| 关键词: 脑缺血 局灶性 电针 内关 百会 NLRP3 ASC Caspase-1 |
| DOI：10.3969/j.issn.1674-070X.2022.08.016 |
| Received:February 08, 2022 |
| 基金项目:湖南省自然科学基金项目（2020JJ8066）；湖南省重点研发项目（2020SK2123）。 |
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| Effect of electroacupuncture at Neiguang (“PC6”) and Baihui (“DU20”) on focal cerebral ischemia-reperfusion injury through NLRP3/ASC/Caspase-1 pathway in rats |
| WANG Qiang,WANG Yue |
| (Hunan Brain Hospital, Changsha, Hunan 410007, China;The First Hospital of Hunan University of Chinese Medicine, Changsha, Hunan 410007, China) |
| Abstract: |
| Objective To investigate the effect and mechanism of electroacupuncture at Neiguan ("PC6") and Baihui ("DU20") on focal cerebral ischemia-reperfusion injury in rats through NOD-like receptor thermal protein domain associated protein 3, (NLRP3)/apoptosis-associated speck-like protein containing CARD (ASC)/Caspase-1 pathway. Methods Thirty Sprague-Dawley rats were randomly divided into control group, sham operation group, model group, nimodipine group and electroacupuncture group, with 6 rats in each group. Middle cerebral artery occlusion (MCAO) was performed in the model group, nimodipine group, and electroacupuncture group. After success, the nimodipine group was given nimodipine 10.8 mg/kg intragastric administration, while electroacupuncture group was given Neiguan ("PC6") and Baihui ("DU20") acupuncture, the control group and the sham operation group were given 0.9% normal saline by gavage for 14 d. Longa score was used to evaluate neurological function. The level of interleukin-1 beta (IL-1β) in serum was measured by ELISA and the expression of NLRP3, ASC, Caspase-1 was detected by immunofluorescence. Results After modeling, Longa scores of model group, nimodipine group and electroacupuncture group were significantly higher than those of control group and sham operation group (P<0.05), after intervention, Longa score in nimodipine group and electroacupuncture group was lower than that in model group (P<0.05), but there was no significant difference in Longa score between nimodipine group and electroacupuncture group. After intervention, the content of IL-1 β in model group was higher than that in control group and sham operation group (P<0.01), and the content of IL-1 β in nimodipine group and electroacupuncture group was lower than that in model group (P<0.05). After intervention, the expression of ASC, Caspase-1 and NLRP3 in model group was significantly higher than that in control group and sham operation group (P<0.01). Compared with model group, the expression of ASC, Caspase-1 and NLRP3 in nimodipine group, electroacupuncture group was significantly lower (P<0.01). Conclusion Electroacupuncture at Neiguan ("PC6") and Baihui ("DU20") can improve the motor function of rats with focal cerebral ischemia-reperfusion injury, which may be related to regulating the NLRP3/ASC/Caspase-1 signaling pathway and decreasing the expression of IL-1β. |
| Key words: cerebral ischemia focal electroacupuncture Neiguan ("PC6") Baihui ("DU20") NLRP3 ASC Caspase-1 |
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